Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"Trypsin inhibitors"”.
-
Establishment of a transformation procedure to study the role of trypsin inhibitors in soybean
The major serine proteinase inhibitors Kunitz and Bowman-Birk-type trypsin are key anti-nutrients responsible for the low nutritional value of raw soy cake, the by product of oil expression from soybean. Traditionally, proteinase inhibitors are eliminated from soy cake through intensive heating, …
-
Sephadex G-25 and Charcoal Separation of Mouse Growth Inhibitors in Soybeans from Trypsin Inhibitors
<p>Rising costs have changes man’s ideals and eating habits. Plant ingredients, because of their relatively low costs, have been increasingly used as a substitute for animal proteins. If these plant ingredients could be directly utilized by man, this would be a more efficient use of energy and …
-
Rapid quantification of trypsin inhibitors in food and feed formulations with electrospray ionization mass spectrometry
… and feed formulations. The important one being trypsin inhibitors. Trypsin inhibitors in food and feed formulations lower the protein supplement and hence the ultimate consequence is the decrease in growth and development of animals. It is therefore, crucial to monitor trypsin inhibitors in …
-
Enhancing Seed Quality and Application of Soybean through High Test Weigh and Low Trypsin Inhibitors
… in its uses due to the antinutritional factor, trypsin inhibitor (TI). This project first worked to identify single nucleotide polymorphisms (SNPs) associated with high test weight through a genome-wide association study (GWAS) as well as determine the relationship between important seed …
-
Molecular characterization of soybean meal trypsin inhibitors and lectins as a basis for developing approaches to mitigate their anti-nutritional effects
… raw form because it contains anti-nutrients like trypsin inhibitors which bind to serine proteases (chymotrypsin, elastase and trypsin) and restrain their digestive functions leading to indigestion and stunted growth. It also contains lectin which binds to intestinal cells containing N-acetyl …
-
Synthesis and expression of the Erythrina trypsin/tissue plasminogen activator (tPA) inhibitor encoding-gene : genetic dissection to correlate the interaction of Erythrina and Soybean trypsin inhibitors with tPA
A trypsin inhibitor had previously been isolated from Erythrina caffra, a member of the Leguminosae family. The inhibitor, Erythrina trypsin inhibitor (ETI), is unique among plantderived inhibitors, in that in addition to trypsin, it inhibits chymotrypsin and tissue plasminogen activator (tPA). ETI …
-
A comparison among lipoxygenase, trypsin inhibitor, and urease enzyme inactivation during blanching of dehulled and whole soybeans
… of inactivation kinetics of lipoxygenase (LO), trypsin inhibitors (TI), and urease (U) enzyme was used to determine the limiting factor for processing. In addition, comparison of inactivation kinetics and protein insolubilization was investigated with regards to type of bean sample; variety of …
-
Effects of genetic selection and processing of soybeans and soybean meal on nutritional quality
… or ultra-low (ESBM-ULT) concentrations of trypsin inhibitor soybeans. Results indicate that the SID of all AA was greater (P < 0.05) in SBM than in all the ESBM, therefore, it is not possible to use these low-trypsin inhibitor beans in diets fed to pigs without heat treatment with only 7 to …
-
The discovery and functional study of a bioactive peptide, QUB-3273, from the skin secretion of the frog, Odorrana schmackeri
… such as neurotransmitters, antibiotics and trypsin inhibitors. To solve the severe antimicrobial resistance, antimicrobial peptide gradually becomes a promising agent. <br/>In this study, the precursor-encoding cDNA sequence of QUB-3273 was identified from Odorrana schmackeri skin secretion. …
-
QUB-1951: a bioactive peptide from the skin secretion of the Chinese piebald Odorous frog, Odorrana schmackeri
… assays, including antimicrobial assay, trypsin inhibition assay haemolysis assay, and cell viability assay. The results showed that this peptide had the ability to inhibit trypsin with Ki value of 1.97 μM, but neither antimicrobial nor severe haemolytic activity were detected. It was the …
-
Assessing and Implementing Value-Added Soybean Innovations in the Soybean Meal and Food Industry
… quality traits and the presence of trypsin inhibitors (TIs) - antinutritional proteins that impair protein digestion in monogastric animals. This study employed a multifaceted approach combining phenotyping, genome editing, and association mapping to enhance soybean quality. First, …
-
The penetration protease of the cercariae of Schistosoma mansoni
… could be totally inhibited by serine protease inhibitors such as diisopropylfluorophosphate or phenylmethylsulfonyl fluoride, but not by the sulfhydryl reagents iodoacetamide or p-chloromercuribenzoate. Typical trypsin inhibitors such as soy bean trypsin inhibitor, trasylol or benzamidine …
-
Morphological and Biochemical Analysis of Variation in Diploid Glycine Tomentella Hayata (2N = 38, 40)
… were examined by two methods: (1) General trypsin inhibitors separated by PAGE and visualized with negative staining, and (2) Bowman-Birk inhibitors (BBI) separated by isoelectric focusing (IEF) and visualized by immuno-staining of Western blots using anti-BBI primary antibodies and a …
-
Physiological responses of the small intestine to nutritional factors and rotavirus infection afflicting young pigs
… protein source for infants. Removal of trypsin inhibitors and partial enzymatic hydrolysis of soy protein source creates an acceptable protein source for young pigs. Experiment 3: Rotavirus infection causes damage of the mucosa in the small intestine resulting in severe watery diarrhea. …
-
Fast flow biopolymer synthesis
… proteins, the disulfide-rich Ecballium elaterium trypsin inhibitor II (EETI-II) and a minimized Z domain of protein A (Z33), is reported. For each protein, the chirality of one amino acid at a time was inverted to generate a series of diastereomers, and study the critical stereocenters of EETI-I …
-
Part-I Characterization of phorbol ester; the predominant toxic component of Jathropha curcas and development of an efficient process for the detoxification of Jathropha curcas meal Part-II quantification of 2-hydroxy-4-(methylthio) butanoic acid and pantothenic acid in bovine serum and sea water matrices
… (PEs), while the major anti-nutritionals are the trypsin inhibitors (TIs). However, chemical characterization of both the jatropha PEs and TIs is at best very sparse. Research described in this dissertation was directed at chemical characterization of phorbol esters (PEs), development of processes …
-
Genetic diversity and detection of Kunitz protein in local soybean varieties
Submitted in partial fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban Institute of Technology, Durban, South Africa, 2003.
-
Molecular cloning, structural characterisation and functional study of peptides from amphibian skin secretion
… peptides (AMPs), anticancer peptides and trypsin inhibitors. Several kinds of AMPs, isolated and characterised from the skin of amphibians, have been studied by researchers to provide novel kinds of drugs and pharmacological agents for humans to treat diseases. <br/><br/>In this study, the …
-
Novel soybean products fed to weanling pigs
… with an emphasis on the effect that trypsin inhibitors have on protein digestion. The apparent (AID) and standardized (SID) ileal digestibility were determined using low temperature and high temperature-processed full-fat soybeans (FFSB) as well as a low temperature and high …
-
Caldolase: an alkaline serine protease from Thermus strain TOK₃
… involved the incorporation of various protease inhibitors (particularly chelating agents) in casein agar plates, the second the inclusion of a variety of native and chromogenic proteins in the agar plates. In conjunction, these methods provided a basis for screening extreme thermophiles for …
Page 1 of 2