Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Triple Quadrupole Mass Spectrometer"”.
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Electron Transfer and Other Reactions Using Atomic Metal Anions
… and reacted with various neutral reactants in a triple quadrupole mass spectrometer. The metal anions were formed via electrospray ionization of the metal-oxalate solutions and form in gas phase between the capillary and the first quadrupole. Neutral gas phase reactants investigated include NO, …
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A new mass spectrometric assay of N8-acetylspermidine deacetylase and partial purification of the enzyme
… pathway.</p><p>The enzyme assay, based on mass spectrometric detection of a specific reaction product following sample introduction by flow injection, was shown to have a sensitivity of smaller than 1 micromolar and typical RSD of 3-10%. The linear range for analyte was from 1 μM to 100 μM, …
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Proteomic insights into the modulation of foetal neurogenesis by the anti-retroviral efavirenz
… molecular changes may be characterised using mass spectrometry (MS). Aims: To optimise an MS-based efavirenz extraction and detection assay, and to investigate efavirenzinduced NES proteomic responses. Methods: A TSQ Vantage triple quadrupole mass spectrometer was employed to optimise targeted …
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Development and Testing of a Rapid Multiplex Assay for the Identification of Biological Stains
… technologies, bioinformatics tool, and tandem mass spectrometry a database of fluid-specific candidate markers will be developed for each body fluid.</p> <p>2. Biomarker Verification – Using the most promising candidates from Phase 1 a targeted multiplex assay will be developed on a Quadruple …
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Development of Mass Spectrometric Methods for the Analysis of Components and Complex Interactions in Biological Systems
… in vitro studies, combined with gas phase mass spectrometry studies, have shown that a stronger binding affinity equates to a greater efficacy of the drug. For the determination of drug:dye complex binding strength coefficients in solution, two methods have been developed by affinity …
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Synthesis and applications of functional magnetic polymer beads; synthesis and mass spectrometry analysis of model peptides
… synthesized, and the sequences were confirmed by mass spectrometry analysis. The yields of the peptides were comparable to those obtained from commercial Rink amide resin. The second part of the thesis describes the synthesis and mass spectrometry analysis of two series of model peptides. One …
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Pharmacocinétique comparative des curcuminoïdes avec ou sans oméga-3 : une étude en chassé-croisé randomisée
… curcuminoids concentration. The study was a triple-blind, randomized, crossover study where participants tested three formulations: 400 mg of curcuminoids powder extract, 400 mg of curcuminoids in rice oil and 400 mg of curcuminoids with 1 g MAG-OM3. Sixteen males and fifteen females consumed …
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The determination of β-endosulfan and endosulfan sulfate in human serum with dialkylphosphate metabolites as urinary markers using LC-MS/MS electrospray ionization
… performance liquid chromatography with tandem mass spectrometry as detection method due to its high sensitivity and selectivity. Chromatographic separation for both bioanalytical methods was achieved by performing reverse phase chromatography on C18 analytical columns. Isocratic elution with a …
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Monoclonal Antibody Therapy: The Development of a Liquid Chromatography Tandem Mass Spectrometry Method to Measure Rituximab Concentrations in Human Plasma
… techniques and liquid chromatography tandem mass spectrometry (LC-MS/MS) as the detection method. This was achieved by employing a bottom-up approach, culminating in the identification of a target peptide that serves as a representative of RTX. Sample preparation started with affinity binding …
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An Investigation into the Use of Dried Blood Spot Analysis in Pharmacokinetic Studies
… of dried blood spot sampling coupled with mass spectrometry detection for drug quantification, and clinically validate the robustness and feasibility of this technique for pharmacokinetic studies in preterm neonates. Dried blood spot methods were developed for application to pharmacokinetic …
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Quantitation of enteroendocrine hormones as metabolic disease biomarkers in human biological matrices by liquid chromatography tandem mass spectrometry
… explored in research. Liquid Chromatography Mass Spectrometry (LC-MS/MS) methods are becoming an alternative approach and analytical technique combines the physical separation abilities of liquid chromatography with specific mass analysis of mass spectrometry. The methods described in this …
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Improvement of Gastroparesis Management By Addressing Challenges in Drug Metabolism: Studies with Metabolite Identification, Reaction Phenotyping and In Vitro Drug-Drug Interactions
… and urine of gastroparesis patients using tandem mass spectrometry. First, the metabolites were identified in in-vitro human subcellular fractions. The knowledge gained in this experiment helped identifying the metabolites in the biological fluids of patients. In total, 12 metabolites including 7 …
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Simultaneous quantification of first-line anti-tuberculosis drugs and metabolites in human plasma
… liquid chromatography (HPLC) with tandem mass spectrometry (MS/MS) detection. Deuterated internal standards for each analyte (AcINH-d4, desRIF-d3, EMB-d4, INH-d4, PZA-15N,d3 and RIF-d3) were used. Mean recoveries of the analytes from plasma were as follows: AcINH 106.5%, DesRIF 123.2%, EMB …
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Discovery of Quinic Acid Derivatives as Oral Anti-inflammatory Agents
… interaction liquid chromatography–tandem mass spectrometry (HILIC-MS/MS) analytical assay. An analog of KZ-41 was used as internal standard (IS). KZ-41 and the IS were obtained by protein precipitation and separated by HILIC chromatography using acetonitrile and water. A triple quadrupole …
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Quantification of pyrethroid and organophosphate metabolites in hair and urine samples of rural children from the Western Cape, South Africa using the two-dimensional gas chromatography-mass spectrometry
… gas chromatography coupled with a time-of-flight mass spectrometry (2-D GCxGC TOF/MS) based method, specifically designed for analysis of compounds in complex matrices such as urine and hair matrices. Furthermore, the 2D GCxGC TOF/MS was available in-house and proved to be less expensive than …
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Applications of metabolomics to study the pathophysiology of adverse pregnancy outcomes
… liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS). Plasma (Cork) and urine (Cork, Auckland) samples were collected at 20 weeks of gestation from pregnant women participating in the SCreening fOr Pregnancy Endpoints (SCOPE) study, an …
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Nuclear PTEN Regulates Thymidylate Biosynthesis and Cellular Sensitivity to Antifolate Treatment
… of de novo thymidylate biosynthesis. Using Mass-Spectrometry, we discovered that MTHFD1 is a top candidate protein interacting with PTEN in human prostate cancer cells. This is the key focus of this paper and will be important background for Chapter 7 and 8 which delve into the thymidylate …