Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Tricarboxylic acid cycle"”.
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Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects
… for de novo synthesis of proteins, nucleic acids and lipids. Yet, a subset tumors harbor genetic mutations in the electron transport chain or tricarboxylic acid cycle that disable normal oxidative mitochondrial function. Importantly, it has been unknown how these cells generate the …
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Molecular and Genetic Relationships of Aromatic Metabolic Plasmids in Pseudomonads
… further dissimilated, through the (alpha)-keto acid pathway (meta cleavage), to acetaldehyde and pyruvate which enter the tricarboxylic acid cycle. The plasmids are large, 40-75 megadaltons (Md) {60-107 kilobases (Kb)}, and encode, in addition to the aromatic growth phenotypes, self-fertility …
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The Tca Cycle As A Nexus of Metabolic Vulnerabilities In Cancer
… suggests that mitochondrial metabolism (tricarboxylic acid cycle (TCA) and oxidative phosphorylation) is imperative for cancer cell growth and proliferation. The scope of the works presented in this dissertation is to explore the importance of mitochondrial metabolism, and primarily the …
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Quantitative analysis of carbon fluxes for fat biosynthesis in wild-type and IRS-1 knockout brown adipocytes
… estimation. Glutamine contributed more to fatty acid synthesis than glucose in WT cells while glucose's contribution was heavier in IRS-1 KO cells. Unlike the straightforward pathway for lipogenesis from glucose, two possibilities for glutamine's route to fatty acid synthesis have been proposed: …
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Identification and analysis of Arabidopsis thiamine pyrophosphate transporters
… the pentose phosphate pathway (PPP) and the tricarboxylic acid cycle; moreover, it is essential for the proper functioning of all organisms. Recently, several steps of the plant thiamine biosynthetic pathway have been characterised, and a mechanism of feedback regulation for thiamine …
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Microbial Degradation of Acetaldehyde in Freshwater, Estuarine and Marine Environments
… via the oxidative decarboxylation steps of the tricarboxylic acid cycle. Acetaldehyde-derived carbon is assimilated into biomass by upregulating the glyoxylate shunt and gluconeogenesis pathway, ensuring carbon is conserved for growth. Using 14C-radiolabelling, rates of microbial dissimilation …
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The Proteomic Response of Gill Tissue in Tidally and Subtidally-Acclimated California Mussels, Mytilus californianus, to Acute Emersion-Induced Anoxia
… However, changes in the abundance of several tricarboxylic acid cycle enzymes (e.g. aconitase, succinate dehydrogenase) suggest that tidally-acclimated mussels are also primed to sense reactive oxygen species (ROS) and limit their production, respectively. These findings are further supported …
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Developing drugs to attenuate succinate accumulation and oxidation
… In addition, the physiological role of the tricarboxylic acid cycle metabolite, itaconate, was investigated. In lipopolysaccharide stimulated macrophages, itaconate has been reported to exert its effects by inhibition of SDH however, I found itaconate was a relatively poor SDH inhibitor, …
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Bio-Nano Interfaces: Enzyme Immobilization for Biomimetic Energy Harvesting
… two methods for co-immobilization on consecutive tricarboxylic acid cycle (TCA) enzymes were investigated. Encapsulation using deacylated chitosan (chit) and tethering through amide bond linkage with 1-pyrenebutanoic acid, succinimidyl ester (PBSE) were used to immobilize porcine heart malate …
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Directed evolution of antimutator E. coli
… of these strains are in genes involved in the tricarboxylic acid cycle and electron transport chain (sucD and sdhA). These alleles are shown to reduce oxidative stress. Chapter 4 demonstrates results from another PResERV experiment using a clean-genome E. coli strain, MDS42, as the host …
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The Role of Mitochondria in the Development of Insulin Resistance and Type 2 Diabetes
… the biological pathways of glycolysis, the tricarboxylic acid cycle, and mitochondrial translation all show evidence of pathway enrichment. These findings demonstrate for the first time, potential associations between these pathways and T2D susceptibility in European and African American …
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Secondary problems caused by the oxyr response to hydrogen peroxide stress in Escherichia coli
… seem to arise due to defects in several amino acid biosynthetic pathways and the tricarboxylic acid cycle. Interestingly, these defects do not seem to be due to iron starvation, as evidenced by the lower activities not only of the iron-dependent enzyme fumarase A but also of the …
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Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… pathways, including Re-type citrate synthase in tricarboxylic acid cycle and citramalate pathways as an alternate route for isoleucine biosynthesis, have been identified in Thermoanaerobacter X514 and other environmental microorganisms. Via the same approach, the utilizations of diverse …
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Mathematical modelling of mammalian mitochondrial metabolism
… the cardiac muscle. The mammalian liver-specific tricarboxylic acid cycle (TCA) model developed within the Cell Systems Modelling Group (CSMG) was used to study and understand the basic functional properties of a tissue-specific TCA cycle model system and to form a knowledge base. Based on this …
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Development of imaging techniques to assess tumour cell death
… post-treatment. Fumarate, an intermediate in the tricarboxylic acid cycle, is hydrated in the reaction catalysed by the enzyme fumarase to produce malate. With loss of plasma membrane integrity in dying cells fumarate can rapidly gain access to the enzyme. By measuring increased malate production …
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Development of a Pediatric Model of Nafld in Neonatal Iberian Pigs
… 10<sup>4</sup> cfu/mL) contained <em>Pediococcus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum and Bacillus amyloliquefaciens</em>. Body weight was recorded every 3 d. Serum markers of liver injury and dyslipidemia were measured on d 40 and 65 at 2 h post feeding. Fasting leptin, …
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Targeting Coenzyme A Biosynthesis for Antifungal Development.
… several key metabolic pathways including fatty acid oxidation and synthesis, the tricarboxylic acid cycle, and sterol biosynthesis. Nearly all organisms must synthesize their own CoA from pantothenic acid (vitamin B5-PA) through a five-step sequence of reactions requiring ATP and cysteine. …
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Study of Erythroid Antioxidant Depletion in Childhood Obesity
… of oxidation/nitrosylation products of nucleic acid, proteins, and lipids), various pathways related to energy metabolism (e.g., glycolysis, pentose phosphate pathway, lipid ß-oxidation, tricarboxylic acid cycle), homeostasis of amino acids (particularly branched-chain and aromatic amino acids), …
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Functional analyses of Plasmodium Falciparum primary metabolic genes
Exploitation of plasmodial genes as drug-targets is important but their primary metabolic genes have been neglected and widely thought to be cryptic. However, datamining the plasmodial genome revealed DNA sequences that potentially support a network of glycolytic and TCA pathways. To question the …
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Investigation into Tissue-Specific Mechanisms of Mitochondrial Dysfunction: Models of SUCLA2 Deficiency and a Screen for Potential Genetic Modifiers
With no currently effective treatments available, mitochondrial diseases are one of the most common forms of inherited multisystem disease. Primary disorders of the mitochondria affect an estimated 1 in 4,300 people with typical onset in early childhood. Mitochondrial disorders are classically …
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