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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Transposon mutagenesis"”.
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… novel drug targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony morphology and mycobacteriophage resistance, was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating …
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Development of broadly applicable transgenic tools for the transposon mutagenesis of the red flour beetle, Tribolium castaneum
… markers, multi-component systems, such as transposon-based insertional mutagenesis, can now be introduced to various insect species. To drive the transposase gene for transposable element remobilisation, suitable promoters are needed. The broadly conserved thermotolerance factor, Hsp70, is …
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Whole-genome transposon mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria
… To this end, a combination of whole-genome transposon (Tn) mutagenesis and next generation sequencing (TnSeq) was applied in M. smegmatis ΔmetE, a gene-deletion mutant in which the cobalamin-independent methionine synthase is inactivated, rendering the cobalamin-dependent isoform, MetH, …
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Transposon Mutagenesis Facilitates Discovery Of Genotype-Phenotype Associations and Functional Interrogation of the <i>Mycobacterium Kansasii</i> Genome
… demonstrates the functionality of a phage-based transposon (Tn) mutagenesis system in <em>Mk </em>by generating and screening a library of random insertion mutants for altered macrocolony morphology. In this screen, we identified 41 mutants exhibiting unique phenotypes, among them one carrying …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… CR101 for an efficient integration system, a transposon mutagenesis system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library preparation and sequencing using Oxford Nanopore Technologies (ONT) nanopore Sequencing. Mobile genetic elements …
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Investigation of growth and stress tolerance characteristics of Cronobacter spp.
… by measuring light emission. Following this a transposon mutagenesis library was created in one of the Lux-tagged strains of Cronobacter sakazakii. This library was screened for mutants with affected growth in milk. The majority of mutants identified were associated with amino acid metabolism. …
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Characterization of novel genes involved in cell envelope function in Rhizobium leguminosarum
… in cell envelope function through the use of transposon mutagenesis, and screening for growth sensitivity to peptide-rich media, has been previously successful. Many of these genes are annotated as hypothetical genes of unknown function and it is unclear of how they are integrated in a cell …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… Furthermore, two different experiments using the transposon mutagenesis strategy were carried out in order to screen for B. subtilis mutants able to increase secretion of α-amylase. Transposon mutagenesis was performed with the mariner-based transposon to inactivate gene(s) whose product might …
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Novel regulators of antibiotic biosynthesis in Serratia sp. ATCC 39006
… Novel regulators were sought through random transposon mutagenesis, using the wild type (WT) as parental strain and the prodigiosin production phenotype (red pigment) for screening of mutants. Colonies displaying altered pigmentation were thus selected. The transposon insertion effects on …
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Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
… Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole …
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Regulating rsmA Expression in Pseudomonas aeruginosa
… of<em> rsmA</em> is not well elucidated. Transposon mutagenesis was performed on P. aeruginosa containing a transcriptional <em>rsmA-lacZ</em> fusion to answer this question. Mutants were screened via β-galactosidase assay and transposon insertions identified via arbitrary PCR. A probable …
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Association of <i>glpQ</i> with serum-resistance in <i>salmonella typhimurium</i>
… tagged by Tn<em>phoA</em> insertional transposon mutagenesis. <em>S. typhimurium</em> strain EM876 has a chromosomal Tn<em>phoA</em> insertion and reduced serum-resistance. Inverse PCR and subsequent DNA sequence analysis reveal the insertion to be in the gene <em>glpQ</em>. …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… activities reside on the same polypeptide. Transposon mutagenesis using Tn5supF localized the celD gene within the insert of FD1-1 and supported the zymogram results that the cellulase and xylanase activities were located on a single polypeptide. DNA sequence analysis of celD revealed an …
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Identification of Bacteroides genes involved in Metronidazole resistance
… emphasis on DNA repair genes. These methods were transposon mutagenesis of Bacteroides, and functional complementation of E. coli metronidazole sensitive mutants with genes from B. fragilis.
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Understanding The Molecular Mechanism Of Manganese Oxidation In Leptothrix Discophora Ss1
… is dispensable to Mn oxidation in Leptothrix. Transposon mutagenesis performed on a [INCREMENT]mofA Leptothrix strain resulted in the isolation of white, non-manganese oxidizing mutants. Mapping of the transposon insertions identified insertions in 4 genes located in 2 regions on the …
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Differential Screening in Immunodeficient Mice Reveals Bacterial Enzymes With Unexpected Roles in Host-Pathogen Interactions
… the strategy of "differential" signature-tagged transposon mutagenesis (STM). By evaluating bacterial mutants for differential virulence in strains of immuno-deficient mice, we identified five enzymes required for in vivo survival in the face of specific immune stresses: four Mtb enzymes …
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Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant
… to be 75·C. acetobutylicum mutants m5 06 were by transposon mutagenesis using the conjugative transposon Tn925::Tn917, not transposon Tn925 as previously thought [Babb. B.L. 1990. B.Sc. (Honours) thesis, University of Cape Town. South Africa]. The spore development and the fermentation profiles of …
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OmpD but not OmpC is involved in adherence of Salmonella enterica serovar Typhimurium to human cells
… OmpC and ompD mutant strains were created by transposon mutagenesis utilizing P22-mediated transduction of Tn10 and Tn 5 insertions into wild-type strain 14028. Fluorescein-labeled wild-type and mutant bacteria were incubated with PKH26-labeled T84 cells at various bacteria-to-cell ratios for …
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Transposon Tn5 mutagenesis in Pseudomonas aeruginosa: a study of Beta-lactamase expression
… class C B-lactamase gene of P. aeruginosa. Transposon mutagenesis was considered an effective method for the identification and subsequent cloning of such regulatory genes. To facilitate identification of Tn5 insertions in P. aeruginosa a transposon Tn5-derivative, Tn5::gen, encoding …
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Characterization of Reduced Phosphorus Metabolism in Alcaligenes Faecalis WM2072 and Xanthobacter Flavus WM2814
… by these organisms were identified using transposon mutagenesis and directed mutagenesis experiments. For A. faecalis these include homologs of the ptxD and htxA genes from Pseudomonas stutzeri WM88, which encode an NAD:phosphite oxidoreductase (PtxD) and 2-oxoglutarate-dependent …
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