Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Transport Protein"”.
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Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia
… is directly involved in a microtubule-dependent transport process called intraflagellar transport (IFT). In order to determine the importance of polaris in the development of neuronal cilia, cilium length and numerical density of cilia were quantitatively assessed in six different brain regions …
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Molecular investigation if the rat jejunal active nucleoside transport protein rCNT1
Active, sodium-dependent nucleoside transport systems have been kinetically described in a number of different mammalian tissues. The recent cloning of several active, mammalian nucleoside transporters belonging to a novel gene family has provided an opportunity to examine both the …
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The Role of Mammalian Lipid Transport Protein ORP1 During Coxiella Burnetii Infection
… 4B secretion system (T4SS) to secrete effector proteins into the host cell cytoplasm. While the CCV membrane is rich in sterols, cholesterol accumulation in the CCV is bacteriolytic, suggesting that C. burnetii regulation of lipid transport is critical for infection. The mammalian lipid …
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Development of pseudosymmetry analysis to identify key residues in transport protein mechanisms
Transport proteins, despite making up approximately 10% of the human genome, are understudied. Multiple computational tools exist to analyse them, but no tool exists to predict which residues are likely to be involved in substrate binding or in the mechanism of the transporter. In this thesis, I …
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Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes
… epithelium contains mRNA encoding for a peptide transporter (s) and to determine di- to octapeptide transport capability, poly(A)+ RNA isolated from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d …
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Isolation and Characterization of an Isoprenoid Transporter Protein in Manduca sexta
Metabolic regulation and transport is a fundamental process in all eukaryotic organisms. Metabolites synthesized throughout the cell must be used in various locations to ensure proper cell function. Isoprenoids are metabolite molecules implicated in cell growth, energy production, and act as …
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Moessbauer Studies of Exchange-Coupled Two-Iron Centers in Proteins (Hemerythrin, Uteroferrin, Epr)
… measurements on three derivatives of the oxygen-transport protein hemerythrin and on the oxidized and reduced forms of uteroferrin, a purple acid phosphatase isolated from porcine uterine fluid. Both proteins contain an exchange-coupled pair of iron atoms coordinated directly to amino acid side …
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Investigating nuclear transport proteins as secreted cancer biomarkers
… analysis identified members of the nuclear transport protein family as significantly upregulated in cervical cancer biopsies compared to normal cervical epithelial tissues. These results were validated at both mRNA and protein levels, and similar upregulation observed in oesophageal cancer. …
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Tissue Distribution of a Peptide Transporter mRNA in Sheep, Dairy Cows, Pigs, and Chickens
… sheep omasal epithelium encoding for a peptide transport protein(s), a 446-bp cDNA fragment was cloned from sheep omasal epithelium RNA. The predicted amino acid sequence of this fragment was 85.8, 90.5, and 90.5 percent identical to rabbit, human, and rat PepT1, respectively. The fragment was …
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The Development of Methods to Account for Physiologic Dynamic Changes and Their Effects on the Pharmacokinetics of Therapeutic Monoclonal Antibodies and other Therapeutics
… a medication can stem from changes CYP enzymes, transport proteins, binding protein expression, organ function, immune reactivity, and health status to name a few; with the focus of this dissertation on the dynamic changes in the ontogeny of MRP2 (an apical liver transport protein) and the …
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Hexose Transport In L6 Rat Myoblasts
… the nature and the regulation of the hexose transport system in the permanent cell line, L6. This rat myoblast line can differentiate in culture to form multinucleated myotubes, but in this study, only the undifferentiated cells were used. Kinetic and inhibition studies suggested that the …
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Development of polymer and lipid materials for enhanced delivery of nucleic acids and proteins
… therapeutics such as nucleic acids and proteins could potentially catalyze the clinical translation of many gene and protein-based therapies. However, progress has been hindered by a lack of safe and effective materials and by insufficient insight into the relationship between key …
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Vitamin B12 Bioconjugates In Pharmaceutical Design
… pathway, in particular that of the enteric B12 transport protein intrinsic factor (IF) and its receptor cubilin, and the expression and exploitation of IF/cubilin in pharmaceutical development.</p> <p>Q1: (Chapter 2) Does an 89Zr-B12 conjugate allow for improved background/noise ratio, relative …
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Field trials to phenotype transgenic tobacco plants with improved RuBP regeneration
… designed to either overexpress the native H-protein of the glycine cleavage system or express the foreign genes for the cyanobacterial bifunctional FBPase/SBPase and algal cytochrome c6 proteins. The bifunctional FBPase/SBPase protein catalyses two steps in the regenerative phase of the …
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Restored iron transport by a small molecule promotes absorption and hemoglobinization: discovery, development, and mechanistic studies
… therapeutics that bind to and inhibit overactive protein function. This has been transformational in the treatment of diseases resulting from an excess of protein function, however, thousands of diseases are alternatively caused by a deficiency of protein function and thus remain incurable. A …
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Investigating the role of nuclear transport proteins secreted by cancer cells
… in our laboratory have shown that the nuclear transport protein Importin 1 (Kpnb1) is upregulated in several types of cancer and is crucial to cancer cell survival. Recent findings from our laboratory showed that Kpnb1, amongst other proteins is secreted into the extracellular space by …
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Cloning, Sequencing and Expression of a Porcine Intestinal Peptide Transporter in a Mammalian Cell Line
Absorption of dietary proteins can be met through the uptake of free amino acids or as small peptides. A peptide transport protein, PepT1, is responsible for the absorption of intact peptides arising from digestion of dietary proteins. PepT1 is driven by a H+-coupled transport system that allows …
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Development of Potent Inhibitors of the Sphingosine-1-Phosphate Transporter Spns2 for the Treatment of Multiple Sclerosis
… derived from its activation of membrane-bound G-protein coupled receptors (GPCRs). The binding of S1P to these receptors (S1P1-5) is closely associated with immune cell development and recruitment. As such, the modulation of S1P-related pathways is of particular interest for the development of …
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Protons and Potentials: How Neurotransmission Influences and is Influenced by pH Transients
… functions, contributing to energy metabolism, protein transport, protein modulation, and ion homeostasis. While usually tightly controlled by systemic and cellular processes, free hydrogen ions can overwhelm endogenous buffering during neurotransmission and can form transient increases or …
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Mechanisms of Copper Deficiency in the Zebrafish Embryo
… mutations in <italic>ATP7A</italic>, a copper transport protein. Despite the critical requirement for adequate copper nutrition and the characterization of key copper transport proteins there remain significant gaps in our understanding of copper metabolism. In order to better understand both …
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