Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Transmembrane helices"”.
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Bioenergetic coupling in P-glycoprotein: determining the relative position, topography and role of transmembrane helices six and twelve
… determined the relative position of two transmembrane helices, six and twelve (TM6 & 12), by measuring the distances between residues. Site directed mutagenesis was used to generate nine double mutants, each containing one cysteine on TM6 and one on TM12. Double mutants were expressed …
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Bioenergetic coupling in P-glycoprotein: determining the relative position, topography and role of transmembrane helices six and twelve
… determined the relative position of two transmembrane helices, six and twelve (TM6 & 12), by measuring the distances between residues. Site directed mutagenesis was used to generate nine double mutants, each containing one cysteine on TM6 and one on TM12. Double mutants were expressed …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… hexamers of connexins, proteins that have four transmembrane helices. The transmembrane helices that line the gap-junctional pore have not been identified, and their identification was the main goal of my dissertation project. To accomplish this goal, I used a combination of molecular biology, …
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Molecular insights into signalling mechanisms within TGF-β superfamily
… complex and to understand the importance of transmembrane helices in TGF-β superfamily receptor signalling, respectively. The results presented here demonstrate that the C-terminus of follistatin stretches back along the surface of the molecule and interacts with its FS1 domain hindering …
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Molecular Dynamics Studies of the *Gating Mechanism of a Mechanosensitive Channel
… surface tension. Under a range of conditions the transmembrane helices flattened as the pore widened. Simulations taking into account the nonhomogeneous distribution of stresses in the membrane have shown what parts of the channel are involved in sensing membrane tension. Steered Molecular …
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Defining Topologically Oriented Transmembrane Helix Contacts in Integral Membrane Protein EmrE by a Modified Two-Hybrid Approach
… throughput assay, constructs of individual EmrE transmembrane helices (TMH) were utilized to perform contact mapping of EmrE dimer structure. The results were compiled to construct a transmembrane helix interaction structure. This structure obtained is novel and identifies inconsistencies from …
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Functional and Structural Studies of the Human Voltage-Gated Proton Channel
… 1950s. These channels all share a conserved six-transmembrane helix topology (S1-S6) in which the first four transmembrane helices (S1-S4) form the regulatory voltage-sensor domain and the last two transmembrane helices (S5 and S6) comprise the ion-conducting pore domain. It was thought that all …
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Ceramide-Induced Alternative Translocation of TM4SF20
The polytopic membrane protein TM4SF20 (transmembrane 4 L6 family 20) is a protein containing four transmembrane helices that inhibits the Regulated Intramembrane Proteolysis (RIP) of the transcriptional factor CREB3L1 (cAMP response element binding protein 3-like 1), a transcription factor …
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Identification of surface accessible proteins in parasitic nematode extracellular vesicles
… EV proteomes and identify proteins containing transmembrane helices, as surface accessible peptides present a clear target with biomarker potential. Our data is necessary in understanding biological function of EVs in recipient cells and unlocking the utilization of EVs as diagnostic biomarkers.
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Insights into the Energetics of Membrane-Bound Peptides : Towards an Understanding of the Structural Organisation of Membrane Proteins
… to screen the helix dipole of membrane-bound helices depending on the proximity and geometry of the aqueous phase relative to the helix termini. As a result, the helix dipole of transmembrane helices is found to decrease with peptide length. The analysis is extended to helices in soluble …
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Structural Investigations of Porcupine and Dispatched-1 in Wnt and Hedgehog Morphogen Biogenesis
… DISP1 engages with HH ligand. DISP1 contains 12 transmembrane helices and two extracellular domains (ECDs) in an open state, unlike its structural homologues PTCH1 and NPC1, whose extracellular/luminal domains adopt a closed state. Wnt signaling is essential for embryonic development and adult …
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Structural Characterization of F-type and V-type Rotary ATPases by Single Particle Electron Cryomicroscpy
… Furthermore, the spatial arrangement of transmembrane helices resolved in subunit I can form two passageways that could provide proton access through the membrane-bound region and is consistent with a two-channel model of proton translocation.
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Are bats evolving more potent antiviral immune systems? Investigating the evolution and antiviral capacity of SERINC3 in Rhinolophus bats
… The SERINC protein family is a collection of transmembrane proteins conserved amongst mammals. SERINC3 and SERINC5 are members of this family that have previously been shown to inhibit viral infectivity in multiple viruses such as SARS-CoV-2, Murine Leukemia Virus, Influenza A and HIV-1. …
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Cryo-electron microscopy studies on ovine mitochondrial complex I
… The membrane part of the complex contains 78 transmembrane helices, mostly contributed by conserved antiporter-like subunits responsible for proton translocation. These helices are stabilized by tightly bound lipids (including cardiolipins). The hydrophilic arm harbours flavin mononucleotide …
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Substrate specificity and functional characterization of sodium/dicarboxylate cotransporters
… My work indicates that different multiple transmembrane helices (TMs) are involved in NaDC1 substrate recognition, with the region of TM 3-4 and the C-terminus required for glutarate while the TM 8-10 region is necessary for adipate transport. Further analysis of these two regions provided …
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Electrostatic Interactions in the Voltage Sensor of Kv7.1 and Iks Channels
… channel Kv7.1 coassembled with the single transmembrane auxiliary peptide KCNE1. Although Kv7.1 forms a functional channel, the properties of Kv7.1 are profoundly modulated by KCNE1. Kv7.1, like all voltage gated potassium channels, is composed of two domains: the voltage-sensing domain …
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Ultrafast 2D IR spectroscopy of membrane peptide systems
… environments. Within these complex membranes are transmembrane proteins which comprise up to 50% of the membrane mass, and are themselves diverse in sequence, structure, and function. Combining two-dimensional infrared spectroscopy (2D IR) and molecular dynamics simulation (MD), this thesis …
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Regulatory and Gating Mechanisms of TMEM16A Chloride Channel
… highly conserved orthosteric binding sites in transmembrane helices (TM) 6-8 opens the permeation pathway formed by TMs 3-7. However, prolonged Ca2+-dependent simulation of TMEM16 channels results in current desensitization or rundown, the mechanism of which is unclear. In addition, recent …
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Crustacean phylogenetic systematics and opsin evolution
… These properties occurred mostly at sites in transmembrane helices and included residues previously identified to affect spectral tuning as well as identifying novel sites.</p>
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Engagement of human host receptors by the antiparasitic drug praziquantel
… pocket localized at the base of the first four transmembrane helices (S1-S4) of these TRP channels. Finally, Chapter 4 describes a Ca2+-independent membrane potential (FMP) assay that can be utilized for screening Sm.TRPMPZQ channel as well as Ca2+-impermeable flatworm ion channels with high …
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