Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Translational Repression"”.
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Evolution of caudal translational repression in higher insects
… Entwicklungsfaktor bicoid (bcd) steuert the translationale Repression von caudal (cad) in Drosophila Embryos. Das bcd Gen kommt im Gegensatz zu cad nur in einer abgeleiteten Gruppe von Diptera vor. Die Expression von cad in anderen Insektenarten muss daher auf andere Weise reguliert sein. …
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A Stress-Induced TRNA Depletion Response Mediates Codon-Based Translational Repression and Growth Suppression
<p>Eukaryotic transfer RNAs (tRNAs) can become fragmented upon various cellular stresses, generating tRNA-derived RNA fragments (tRFs). Though this process has been observed for numerous cellular stresses and in many species ranging from plant cells to yeast and human cells, it is still poorly …
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Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance
… tolerance by suppressing SG formation through translational inhibition of the key SG nucleator G3BP. MAGE-B2 reduces G3BP protein levels below the critical concentration for phase separation and suppresses SG initiation. Importantly, knockout of the MAGE-B2 mouse ortholog or overexpression of …
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Translational regulation by short RNAs in mammalian cells
… in mammals by RNA interference (RNAi) and translational repression. RNAi initiated by exogenous siRNAs results in mRNA degradation whereas many endogenous microRNAs cause translational repression. Some factors which produce siRNAs from double-stranded RNA or microRNAs from hairpin …
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IAP antagonist Grim mediates cell death through divergent pathways
… manner, involving Grim’s C-terminus for translational repression. We discovered that Grim is a target for caspase-mediated cleavage at Grim’s C-terminus (Asp132). Grim loses its only lysine (Lys 136) after cleavage, but acquires a longer half-life due to inability of DIAP1 to ubiquitinate …
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Specificity and mechanism of microRNAs in the regulation of gene expression
… the mechanism and specificity of miRNA-directed translational repression. The term RNAi can be used to encompass a wide variety of gene silencing phenomena. The canonical RNAi pathway, as first described by Fire and colleagues in C. elegans and studied biochemically in Drosophila by Tuschl, …
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A study of mammalian microRNA-mediated repression of gene expression by ribosome profiling
… by base pairing to their target mRNAs to direct repression. In animals, this repression is usually mediated through translational repression and/or mRNA destabilization. In studies that investigate miRNA-mediated repression with reporter constructs or individual endogenous genes, translational …
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Zur Transkriptions- und Translationskontrolle des Gens für Transitionsprotein
… framework of this study, the transcriptional and translational regulation of rat Tnp2 gene were investigated by means of in vitro and in vivo systems. In order to determine the minimal promoter region which could direct the testis- and haploid-stage specific expression of the Tnp2 gene, a region …
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Peripubertal Binge Ethanol, Age and Sex Modulate Microrna Expression in the Ventral and Dorsal Hippocampus of the Adolescent Rat
… noncoding RNAs that regulate gene expression by translational repression. microRNA altered in response to EtOH and puberty in the developing brain. The biogenesis of mature 22-24 nucleotide (nt), single-stranded microRNAs involves Drosha and Dicer enzymatic processing of microRNA precursors. A …
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High intensity exercise induced regulation of the microRNA biogenesis pathway
… gene expression by silencing mRNAs through translational repression and/or transcript destabilization – dynamically respond to exercise and regulate the expression of genes involved in mitochondrial biogenesis. However, the regulatory mechanisms governing miRNA expression itself remain …
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Biochemical and Genetic Analyses of Rna-Protein Interactions
… interaction; however, the mechanism of translational repression is unclear. One model has the coat protein sequestering the SD sequence and AUG start codon in secondary structure while another model suggests that coat protein blocks 16S rRNA access to the RNA by steric masking. A new …
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MicroRNAs in early embryonic development : dissecting the role of miR-290 through miR-295 in the mouse
… developmental regulation of gene expression via translational repression of target mRNAs. Targeted deletion of the miRNA biogenesis machinery in the mouse has demonstrated essential roles for miRNAs during early development. In this thesis, I have examined the role of a family of miRNAs, miR-290 …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… found to complex with mRNA decapping factors and translational repressers.<br/>More importantly, p50Nesp1 associated strongly with microtubules, both in vitro and in vivo, and was required for scaffolding P-body complexes to microtubules. By disrupting P-body-microtubule association with a …
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Biochemical Studies of the ER Resident Kinase PERK and Implications for Local Translational Control
… kinase (PERK) is able to couple ER stress to translational repression and transcriptional reprogramming through phosphorylation of eIF2α. This is achieved through the recruitment of eIF2α by a unique cytoplasmic loop in PERK’s kinase domain. PERK signalling has been shown to play an important …
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Pumilio Regulates the Epithelial-Mesenchymal Transition and Gastrulation During Zebrafish Development
The pumilio family of RNA binding proteins act as translational repressors to control developmental patterning events of invertebrates, germline stem cell maintenance, and neuronal growth through deadenylation, inhibition of translational elongation, and allow access of microRNAs to their targets. …
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Characterization of Factors Controlling Germline Stem Cell Maintenance in Drosophila Melanogaster
… partner Bgcn, functions to antagonize the translational repression mediated by Pumilio-Nanos (Pum-Nos) complexes. Repression of Pum-Nos activity by Bam-Bgcn complexes permits CB differentiation, presumably through the derepression of CB-promoting mRNAs. In this study, I investigated the …
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The role of organelle crosstalk in a Drosophila model of Parkinson’s disease
… ER kinase (PERK). PERK activation leads to translational repression via phosphorylation of eukaryotic translation initiation factor-2 α (eIF2α) as well as activation of activating transcription factor 4 (ATF4). This triggers a transcriptional response, initially promoting cell survival. …
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The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
… regulatory framework and can determine the translational fate of their RNA targets. A wide variety of post-transcriptional regulatory mechanisms are employed during the early Drosophila embryo and it has been established as a system well suited for the study of these mechanisms. In …
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Silencing the host : the role of intronic microRNAs
… predominantly the 3' UTR of mRNAs, lead to translational repression or degradation of the target message, hence controlling important cellular mechanisms, including division, differentiation and death. This key role makes them excellent targets for cancer research. In fact they have been …
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Tumor Suppressor APC and Musashi1: Double-Negative Feedback, Wnt Signaling and Colon Cancer
… that APC mRNA is a target of MSI1 binding and translational repression. Further study has indicated that MSI1 binding also results in stabilization of the APC mRNA, presumably as a rapid response mechanism to repress Wnt signal transduction once the signal is no longer present. Finally, my …
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