Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Translational Fusion"”.
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Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12
… consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a <i>glpD-lacZ</i>- translational fusion.
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Mutagenesis of nifE and nifN from Azotobacter vinelandii
… of particular amino acids for-substitution. A translational fusion between nifN and lacZ was constructed and used to study the regulation of nifEN. This gene fusion was regulated in the same manner as wild type nifN and produced a fusion protein which was enzymatically active with respect to …
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ROLE OF CRC IN THE REGULATION OF ALGINATE IN PSEUDOMONAS AERUGINOSA
… formation and diauxic growth. When a PalgD-lacZ (translational) fusion was tested, the crc mutant showed increased algD expression as predicted for a mRNA-binding repressor. Another Ptrc-algD-lacZ (translational) construct, but missing the upstream promoter (PalgD) elements, also showed increased …
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Calcium -dependent protein kinases are myristoylated and associated with different membranes in Arabidopsis thaliana
… saturated fatty acid that is attached co-translationally to the amino-terminal glycine of a nascent protein. Myristoylation can facilitate membrane binding and/or protein-protein interactions.</p><p>In these studies, Arabidopsis CDPK isoforms AtCPK1, AtCPK2, AtCPK5 and AtCPK6 have been …
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Development of a Dusky kob scFv gene phage display library for the discovery of antibodies to Brome mosaic virus - a proxy for a novel, emerging fish pathogen
… to be expressed on the phage surface through translational fusion with phage coat proteins. Consequently, antibodies expressed by a diverse repertoire of genes coding for the single chain variable fragment (scFv) of immunoglobulin M can be isolated and screened for affinity to a specific …
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Identification of novel regulators of the necrosis-inducing protein (Nip) and carbapenem antibiotic production in the phytopathogen Pectobacterium
… strain CP1000 by exploiting a nip::phoA translational fusion screen. These included mutants with insertions in genes fadA and modB_1, in addition to pstA_2 and phoU which are part of the pstSCAB-phoU operon for phosphate uptake; expA which encodes the response regulator of the ExpS/ExpA …
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… the rate of transcription from a luxI-lacZ translational fusion via b-galactosidase assays in recombinant E. coli. In vitro work was performed with LuxRDN, the autoinducer-independent C-terminal domain (amino acids 157 to 250) of LuxR because purified, full length LuxR is unavailable. …
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Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis
… chlamydial library. Target plasmid contained translational fusion between chlamydial library and a positive/negative selection fusion system (<em>ccdB/CAT</em> fusion/TEV protease). The selection process to identify potential targets for ncRNAs was carried out over two stages. Stage one was to …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… with a library of signal peptides (SPs) and translational fusion partners (TFPs) to optimise surface display of a protein of interest – a significant challenge for many recombinant proteins. This expansion of the MoClo-YTK yeast was validated using several proteins of interest and constitutes …
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Role of the Aspartyl Protease SpoIIGA in the Compartmentalization of Sigma Factor Activation During Sporulation of Bacillus subtilis
… of σE. To accomplish this, a spoIIGA-gfp translational fusion was used. The fusion was introduced into a B. subtilis SpoIIGA mutant (SpoIIGA49) that lacks functional SpoIIGA because of a point mutation, G100R. The mutation is located within the fourth membrane-spanning domain. The …
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Studies of the genome and regulatory processes of Vibrio parahaemolyticus
… for potential CsrA targets, a transcriptional/translational fusion system was developed. Transcriptional and translational fusions to β-galactosidase were created to PdksA, PglgC1 and PtoxR from RIMD2210633. CsrA or CsrB2 was overexpressed in recombinant E. coli containing each of the fusion …
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Roles of Small Rnas and Paralogous Proteins In Bacillus Anthracis Virulence Gene Regulation
… sRNA, suggesting base-pairing interactions. A translational fusion of one of these targets, the secreted metalloprotease <em>inhA1</em>, to GFP suggests XrrA-mediated regulation of protease translation. XrrA appears to mediate base-pairing interactions with the<em> inhA1 </em>RBS via a C-rich …