Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"Translation machinery"”.
-
Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
… mitochondrial dysfunction. 12 3. Mitochondrial translation machinery defect may be a key event in mitochondrial dysfunction in hepatoma cells 14 4. Mt-TMD-related invasive behavior induced by doxycycline in SNU-387 cells 20 5. Claudin-1 regulates Mt-TMD-induced hepatoma cell invasiveness. 26 6. …
-
Mitochondrial mRNA Translation is Required for Maintenance of Oxidative Capacity
… this may be through targeting the mt-mRNA translation system. Purpose: This investigation’s purpose was to characterize disruptions in mt-mRNA translation machinery in multiple forms of cardiomyopathy and to determine if mitochondrial mRNA translation initiation factor (mtIF2) is necessary …
-
Genetically encoded division machinery for cell free synthetic biology
… parts of the Escherichia coli cell division machinery inside of water-in-oil emulsion compartments and synthetic phospholipid vesicles. The system was built with DNA and purified transcription and translation machinery housed in a compartment. A particular emphasis was placed on FtsZ, a …
-
Origin of viruses revealed by the genomic study of protein domain structures
… viruses with complex proteomes, remnants of translation machinery and virus-specific parasites have raised important questions about their origin. Evidence advocates for their inclusion into global phylogenomic studies and their consideration as a distinct and ancient form of life. Here we …
-
Investigating the translational and metabolic reprogramming of the bone marrow niche in myeloid malignancy
… cells surviving chemotherapy rely on high mRNA translation and their microenvironmental metabolic support to drive relapse. However, the role of translational reprogramming in the niche was, until now, unclear. This study found that relapsing AML cells increase translation in their bone marrow …
-
eIF4E Phosphorylation Balances Cap-dependent and Cap-independent Translation Initiation
<p>Signaling pathways converge on the translation machinery and influence protein synthesis globally or specifically on certain classes of transcripts. The experiments described in this thesis focus on regulation of translation initiation through the cap-binding protein eIF4E. </p><p>Aberrant …
-
Regulation of Mnk1 by p38α MAPK in Stress Mediated Translation Initiation
… pathways control protein synthesis by modulating translation initiation factors. Map Kinase Integrating Kinase 1 (Mnk1) relays signals to its major downstream target eIF4E. Activation of Mnk1 and subsequent phosphorylation of eIF4E results in changes in translation rates for subsets of mRNAs. Both …
-
Dynamics of Hairpin Ribozyme
… RNA then carries the genetic information to the translation machinery and participates in the process of protein synthesis. Using a set of four nucleotides, RNA is able to fold into complex structures with enzymatic power. This has led us to the speculation that RNA catalytic functions preceded …
-
Investigating the TrkB Interactome during Development and in Neurodegeneration
… and Robo2 were shown to interact with the translation machinery. Both the protein and mRNA interactions of these receptors help them to define which proteins are locally synthesized in response to stimulation. BDNF likewise regulates the local nascent proteome with high spatiotemporal …
-
A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
… there are regulatory steps on the level of translation that can confound correlation between mRNA abundance and protein synthesis rates. Here, I improve upon a proteomics based method for measuring newly translated proteins, biorthogonal non-canonical amino acid tagging (BONCAT), and adapt …
-
Technological advancements towards paper-based biomolecular diagnostics
… fundamentally require transcription and translation, limiting their applicability to cellular contexts In vitro cell-free expression systems that contain transcription and translation machinery provide the environment necessary for biologically-based technologies to function independently …
-
Evolution of Protein Structure
… are one of the major protein components in the translation machinery. These essential proteins are found in all forms of life, and are responsible for charging their cognate tRNAs with the correct amino acid. The evolution of the tRNA synthetases is of fundamental importance with respect to the …
-
A Role for BDNF-Trkb Signaling in the Modulation of Superoxide Dismutase-1 Expression
… assay, and inhibitors of the proteasome and translation machinery provide evidence that in both the acute and chronic phase, BDNF-TrkB signaling is modulating SOD1 expression at the level of translation. Taken as a whole, these data demonstrate that BDNF-TrkB signaling is involved in the …
-
Adaptive Mechanisms of an Estuarine Synechococcus based on Genomics, Transcriptomics, and Proteomics
… a global down regulation of photosystems and the translation machinery. From the stress response studies seven chromosomal toxin-antitoxin (TA) genes, were identified in CB0101, which led to the discovery of TA genes in several marine Synechococcus strains. The activation of the relB2/relE1 TA …
-
Luminescence-Based MicroRNA Detection Methods
… the cell cytoplasm where they regulate protein translation either through direct cleavage of mRNA, or indirect inhibition through binding to mRNA and disrupting the protein translation machinery. Recently, miRNAs have gained much attention due to their implication in numerous diseases and …
-
Insights into the regulation of mTOR signaling and the consequences of pharmacological inhibition
… availability, and connects to the protein translation machinery through its substrates S6K and 4E-BP1. Additionally, S6K can suppress insulin signaling, establishing a negative feedback loop to PI3K. Consistent with its role in cell growth, derangements in mTOR signaling are increasingly …
-
Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… specialized ribosomes, capable of finely tuning translation of selected mRNAs, do exist. Multiple types of ribosomes are defined by several elements, such as their composition, and their association with different proteins, known as ribosome-associated proteins (RAPs). In particular, these …
-
Elucidating the Roles of Eukaryotic Initiation Factors Involved in DAP5 Mediated Translation
<p>Translation initiation in eukaryotes is a highly regulated process essential for accurate protein synthesis. It is a dynamic process that involves a complex interplay between messenger RNAs (mRNAs), ribosomal subunits, and a host of initiation factors, ensuring precise start codon selection and …
-
Rewriting the genome of Escherichia coli
… with parallel efforts to engineer the protein translation machinery. Recoding genomes is subject to both biological and technical challenges. First, synonymous codon choice genome-wide is not trivial, and identifying suitable synonymous replacements is challenging. Second, synthetic DNA pieces …
-
Regulatory consequences of mRNA Poly(A)-Tail length changes
… bind these features increase the stability and translation of an mRNA. The influence of the poly(A) tail on translation was discovered decades ago, but primarily with regard to the idea that an mRNA with a poly(A) tail is better translated than one without. The influence of differences in tail …
Page 1 of 2