Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"Transient transfection"”.
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Extended transient transfection by repeated delivery of in vitro-transcribed RNA
… the rate at which cells recover from ivT-RNA transfection by combined knockdown of innate-immune-related genes was developed. Using this technique a high, approximately steady-state level of protein expression was transduced in MRC-5 human fetal-lung fibroblasts by repeated transfection with …
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Identification and Characterization of the Cis-acting Elements Around the Murine cd4 Cnhancer
… 31 deletion method and then analyzed using the transient transfection, chloramphenicol acetyltransferase (CAT) assay. Two novel <em>cis</em>-acting elements with silencer function were discovered by Bal 31 deletions. Their silencer functions were further confirmed by subcloning them at different …
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Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
… to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native …
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Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
… a HisMax plasmid and expressed at low levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four construct sequences into the IGF-1 HisMax plasmid, and to insert a secretory sequence into all four construct plasmids. A stable cell line selection in 293 …
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Cell tropism of maedi-visna virus
… context of transcription of a reporter gene in transient transfection. The aim of this study was to find where in the replication cycle of MVV without duplication in the LTR the barrier is. This is done by comparing reverse transcription, integration and mRNA synthesis between virus strains with …
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Identification of novel target genes for the plasticity-related transcription factor Zif268
… in response to increased levels of zif268 by transient transfection in PC-12 cells and NMDA stimulation in primary cultured cortical neurones. In addition, ubiquilin mRNA and protein levels were also downregulated within the same experimental paradigms, implying that both gephyrin and …
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Cell type-specific regulation of the chicken tyrosinase promoter
… expression in melanocytes and RPE cells. Transient transfection experiments were carried out in which reporter constructs, consisting oftyrosinase promoter deletion fragments linked to a luciferase reporter gene, were introduced into melanocytes, RPE cells and a non-pigmented cell line. …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… facilities using Agrobacterium-mediated transient transfection of plants. However, transfection methods suitable for open field applications are still desirable as a cheaper alternative. Biosafety concerns related to the use of recombinant agrobacteria in an industrial transfection …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… Cloned E2BP expressed in human fibroblasts by transient transfection displayed similar EII binding and activating characteristics as native E2BP in hepatocytes. This is the first report of the interaction of a hnRNP-binding protein with a viral enhancer element. The concept that …
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Studies of Estrogen Receptor's Roles in Tamoxifen-Induced Apoptosis and the Regulation of Gene Expression
… in ERalpha. To avoid potential artifacts due to transient transfection and use of artificial estrogen response element (ERE)-containing reporter genes, I isolated stably transfected cell lines that express the ERalphaS118A phosphorylation site mutant. Using stably transfected HeLa cell lines with …
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Inhibition of IFN-[gamma] promoter function by site-specific methylation
… is sufficient to inhibit promoter activity in transient transfection assays.
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A Novel Phytoestrogen that Acts as an Agonist for Human Estrogen Receptors.
… 4EM on ER-alpha and -beta activity, I performed transient transfection assays and showed that 4EM activates ER dependent gene transcription in a dose dependent manner in both ER subtypes. Further, 4EM- mediated transcription in ER-alpha, like estrogen, was enhance in the presense of …
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Evidence that ARNT plays a role in the regulation of the immunoglobulin heavy chain enhancer and identification of a putative ARNT ligand
… activation at the IgH enhancer by Pan-1 in transient transfection assays by cotransfection of the multimerized murine form of the IgH enhancer elements 1-1E2, !-LE3 , and 1-1ES upstream of a luciferase reporter gene, a rodent Pan-1 (human homolog E47) expression vector, and an ARNT …
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Characterization of downstream target genes regulated by ABF-1 in different states of B cell development
… lines or B cell lines that overexpress ABF -1 by transient transfection experiments, we discovered many cellular genes that change in their transcriptional profiles in response to ABF -1 expression. Based upon the analysis of genes being affected following ABF-1 induction, our results support the …
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Systems Engineering for Viral Vector Manufacturing
… short of meeting current and future demands. Transient transfection via triple transfection of plasmids is the most common manufacturing platform for the clinical stage due to its flexibility in changing the plasmids and transgene. Triple transfection has low yields and low fraction of full …
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Role of Dimerization in Binding of Estrogen Receptor to the Estrogen Response Element
… wild-type ability to activate transcription in transient transfection assays and several potential mechanisms are proposed to account for this observation.
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Towards the establishment of a highly effective adeno-associated virus production by leveraging next generation sequencing and transcriptomics in HEK293 cells
… exhibited a decreased nutrient consumption after transfection, with the exception of selected compounds such as glutamine. Among the analyzed nutrients, no obvious limitation could be identified. This suggests that rAAV productivity is not inherently linked to nutrient abundance, but rather to …
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Interrogating regulated intramembrane proteolysis of IGF-1R, and its role in cell migration
… of IGF-1R and the production of its CTF via transient transfection of HEK293T cells with plasmids expressing either wild-type or K1003R kinase dead mutant forms of IGF-1R C-terminally tagged with GFP. We subsequently identified receptor kinase activation as a potential driver of IGF-1R RIP …
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Vimentin Overexpression Contributes To the Biological Properties of Metastatic Head and Neck Cancer Cells
… through a basement membrane substitute. Using transient transfection assays, vimentin promoter activity was determined in HN12 cells to define regulatory elements important for controlling vimentin upregulation in the absence or presence of EGF and TGF-β. Taken together, the data indicate that …
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Role of the Intron 13 Polypyrimidine Tract in Soluble Flt-1 Expression
… shown to produce both forms of Flt-1 mRNA after transfection into HEK293 cells. To gauge the strength of the native splicing signals in intron 13 of Flt-1, a series of point mutations were made in the polypyrimidine tract using pFIN13. After transient transfection, the levels of Flt-1 and sFlt-1 …
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