Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Transient Kinetics"”.
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
Crystallization experiments and transient kinetic methods were used to characterize the first 75 amino acid segment of smooth muscle myosin light chain kinase (MLCK N1-75). As of now, the exact mechanism by which MLCK interacts with actomyosin complexes in smooth muscle is not well-understood …
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Dynamic Self-Assembly of Magnetic Colloidal Particles
… a rotating magnetic external field, we cause a transient dynamics from a static to the dynamic equilibrium state. The first question addressed in this thesis is: what is the physics that determines the transient folding pathway from one to the other equilibrium state? Dynamic magnetic fields …
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Myosin structural dynamics: mechanistic insights and therapeutic technology developments
… Our lab has developed a strategic tool called transient time-resolved FRET, (TR)2FRET, to measure directly, with sub-nanometer and sub-millisecond resolution, the structural and biochemical kinetics of muscle myosin. This tool allows us to directly determine how myosin’s power stroke is coupled …
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DROSOPHILA CLASS-I MYOSINS THAT CAN IMPACT LEFT-RIGHT ASYMMETRY HAVE DISTINCT ATPASE KINETICS
… organismal levels. We performed steady-state and transient kinetics to identify differences in key steps of the paralogs’ ATPase cycle, and performed biophysical assays for validation. We discovered myo1C has a rate-limiting phosphate release followed by slow MgADP release, dwelling in the cycle …
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The molecular basis of nucleotide recognition for T7 DNA polymerase
… by a combination of site-directed mutagenesis, transient kinetics and crystallography. One flexible segment (gly-ala-gly) within the fingers domain was mutated to (ala-alaala). The kinetic analysis on this mutant showed that the mutations decreased the forward rate of the conformational change …
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Kinetics and Mechanism of Ozone Decomposition and Oxidation of Ethanol on Manganese Oxide Catalysts
… test the validity of the proposed sequence. The transient kinetics were found to be consistent with the steady state kinetics, and both were well represented by the two-step sequence, indicating that the proposed reaction sequence accurately described the mechanism of decomposition. The surface …
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Non-Steady-State Catalyst Characterization with Thin-Zone TAP Experiments
… microreactor which allows monitoring of reaction kinetics for a well-defined and spatially uniform catalyst state with resolution on the millisecond scale. In the TZ TAP reactor, a narrow catalytic sample is packed between two inert zones in order to minimize macroscopic concentration and …
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Getting to the Point: Bridging the Gap between Simple and Complex Catalytic Systems using Temporal Analysis of Products (TAP)
… in structure and to understand their reaction kinetics, researchers often study simpler surfaces such as single crystals using surface science techniques. This introduces a well-known problem in the field of catalysis commonly referred to as the "pressure and materials gap." Typically, …