Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Transgenics"”.
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CANADIAN CONSUMER PERCEPTION OF GENOME-EDITED FOOD PRODUCTS
… is potentially more socially acceptable than transgenics due to the possibility to add, delete, or alter specific parts of the DNA sequence without adding foreign genetic material. This thesis examines consumers’ perceptions of food produced using genome editing techniques. To accomplish this, …
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Functional Analysis of AtVAMP714 gene in Arabidopsis
… were significantly reduced. Dominant negative transgenics of AtVAMP714 were also created as a method of knocking out the function of the protein and F2 generation plants were analysed for developmental defects. Consistent with the above results the dominant negative transgenics showed a short …
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Identification and characterisation of HLA-DRB1*0101 restricted CD4+ T cell epitopes in the major grass pollen allergens Phl p 1 and Phl p 5
… guided epitope mapping and human HLA-DR1 transgenics. I investigated recognition of HLA-DR1-restricted CD4+ T cell epitopes in allergic and non-allergic individuals and characterised epitope-specific CD4+ T cells, according to cell surface markers and cytokine production.<br/>HLA-DR1 …
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Improving barley for biofuel production – investigating the role of 4CL and CCR in the lignin biosynthesis pathway
… traits were apparent in the 4CL and CCR transgenics. Selected 4CL and CCR transgenics had improved saccharification yield after using three different pretreatment methods, which is a desirable feature for biofuel production.
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Characterization and Expression of Plant Defense Genes in Peach and Tomato
… the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines …
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Regulation of Rubisco in Arabidopsis Thaliana Rca(-) Complemented With Arabidopsis Rubisco Activase Engineered at an ATP Binding Site
… is expressed in the wild type and not in the transgenics, the results suggested that the 46kD isoform of Arabidopsis Rubisco activase may be a light regulatory component of Rubisco activation.
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Representations and Transformations of Odor Information in the Mouse Olfactory System
… response to odors other than acetophenone in M71 transgenics, a wide variety of odorants are able to evoke mitral and tufted cell activity in these mice. Furthermore, the number of cells responding to these odors as well as the magnitude of these odor-evoked responses are higher in M71 transgenics …
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Functional analysis of Poplar genes regulating flowering and vegetative growth
… I studied INRA 717-1B (P. tremula x P. alba) transgenics with an artificial microRNA (AmiRNA) downregulating PopNAC154 and PopNAC156 (AmiSND2 trees). In a field trial, AmiSND2 trees showed higher mean height and diameter than wild-type (WT). We also observed that AmiSND2 transgenics showed …
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A Genome Scan for Quantitative Trait Loci in Dairy Cattle
… marker-assisted selection, therapeutics, or transgenics.
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Modification of the Bovine Genome for the Large-Scale Production of Human Serum Albumin
… on blood bank supplies. Previous bovine transgenics, which simply express ectopic rHSA in the milk, have not proved commercially viable. This is primarily due to the presence of endogenous bovine serum albumin (BSA) in the milk, a highly conserved ortholog of HSA, which necessitates a …
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Cluster analysis of soybean pathogen-responsive genes and functional characterization in Arabidopsis
… resistant varieties and possibly used to develop transgenics with enhanced resistance.
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Generation of Transgenic <i>Medicago Sativa</i> Overexpressing "<i>Osmotin-Chitinase</i>" Gene Chimera
… for screening. Further screening of the positive transgenics was performed using PCR. Southern hybridization was carried out for further confirmation of successful transformation. Transformed shoots will be grown on the root inducing medium for developing into plantlets which would then be …
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Development of Tools to Assess the Effects of Lunasin on Normal Development and Tumor Progression in Drosophila Melanogaster
… transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. Progeny were assessed for phenotypic alterations and no phenotypic abnormalities were observed in tissues expressing EGFP-Lunasin, supporting current studies that show Lunasin does not affect normal cells. …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… modified rodent mice (iPLA2β knockouts and transgenics) also demonstrated the involvement of iPLA2β in alternative splicing. Together, these observations indicate that iPLA2β plays an important role in the regulation of pre-mRNA splicing of key apoptotic factors. Our findings therefore …
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Understanding How Sequence Differences Among Glutamate Transporters Might Contribute to their Differential Function in Glutamate Clearance in C. elegans
… their differential sequence/structure. We use transgenics and optogenetics in intact animals to study this relationship in the transparent nematode C. elegans. We examine sequence differences between different nematode GluTs and ask if these variations might have functional consequences, by …
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Life in the woods : production and consumption of the urban forest
… to the original tree. The controversial field of transgenics further complicates matters as scientists now work to genetically modify trees for improved yield and performance. Many environmentalists argue that the risk of infecting native tree populations is too great, while others see potential …
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Functional analysis of novel protein-protein interactions involving ROP GTPases in Arabidopsis thaliana and Populus trichocarpa
… one of the interacting partners separately. Transgenics altered in expression of PB15 and/or PB129 were prepared in Populus and characterization of transgenic trees will be performed in greenhouse and field. In addition to DUF620 family proteins, ROP11 also interacts with the COP9 subunit …
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Characterizing global gene expression and antiviral response in Frankliniella occidentalis infected with Tomato spotted wilt virus
… feeding bioassays to explore the effect of these transgenics on thrips fitness indicate a need for further description of thrips dsRNA uptake. In total, my research contributes new basic knowledge underpinning the complex and dynamic relationship between thrips vectors and the plant viruses they …
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Functional analysis of soybean PR-10 genes
… for basic research and possible development of transgenics with enhanced resistance.
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Engineering a cyanobacteriochrome two-component system into a synthetic, light-controlled gene expression system for plants
… in *Arabidopsis thaliana* and generate stable transgenics. I aim to target GA20OX1, a gibberellin (GA) synthesis gene with the aim of modifying GA patterns visualised by the FRET-based biosensor nlsGPS1 developed by our group. To speed up *in planta* testing of new Highlighter variants, I …
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