Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 132 for “"Transgenic plants"”.
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Transgenic Plants, Current State, and Influence on the Environment
… cells. Public concerns about the impact that transgenic plant might have on the environment have partly slowed down the large-scale use of transgenic plants. These concerns are discussed. Some suggestions regarding the releasing of the transgenic plant under field conditions are also discussed.
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The development of transgenic plants resistant to cucumber mosaic virus and tobacco necrosis virus
… on yields of agriculturally important crop plants. The complete cDNA sequences of CMV-Wemmershoek (CMV-Wem) coat protein (CP) and TNV-F5P CP genes were cloned and subjected to sequence analysis. CMV-Wem is closely related to CMV-WL and CMV-Q, and therefore falls into CMV subgroup II. Similar …
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An investigation into the high level production of proteins in tobacco using transgenic plants or viral vectors
… constructs were inoculated onto CMV infected plants. Inoculated plants were monitored for CAT expression. No CAT could be detected in total protein extracts of inoculated plants. No CAT mRNA could be detected in northern blots of total RNA extracted from inoculated plants. The vector …
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The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants
The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern …
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Functional analyses of tomato 3-hydroxy-3-methylglutaryl coenzyme a reductase (HMGR) genes in transgenic plants engineered for altered HMGR expression
… and antisense inhibition of hmg1 and hmg2 in transgenic plants. Activity of the hmg2 promoter is developmentally regulated showing expression in seedling cotyledons and hypocotyls, in trichomes, and in reproductive tissues including pollen, stigmas, ovules, petals and mature seeds. hmg2:GUS …
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Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene
The Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern …
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A szárazságtűrés élettani hátterének vizsgálata Arabidopsis modellnövényen és transzgenikus szárazságtűrő növények előállítása = Examinations of the Physiological Background of Drought Hardiness in Arabidopsis and Production of Transgenic Plants
A kultúrnövények szárazságtűrése napjainkban igen égető probléma. Az öntözéshez használható édesvíz-készlet folyamatos csökkenése, a csapadékhiány és a globális felmelegedés egyre nagyobb szárazság-stressznek teszi ki termesztett növényeinket. Ebben a helyzetben fontossá vált a növények vízfelvevő …
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Studies on rice transformation and the use of transformed plants [electronic resource]
… methods to determine the sensitivity of transgenic rice plants to hygromycin B and Liberty were developed, tested and used in this research. Four homozygous transformed plants were selected to make reciprocal crosses with their non-transformed parent cultivars Taipei 309 and Nipponbare. …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… and their hosts are constantly evolving. When plants produce a defense system against viral infection, viruses develop means to overcome this host response. Soybean [Glycine max (L.) Merr.] plants infected with Bean pod mottle virus (BPMV) alone develop acute symptoms that nearly disappear as …
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Genetic Improvement of Switchgrass Cell Wall Content, Leaf Angle and Flowering Time
… is involved in monolignol biosynthesis. Stable transgenic plants were obtained with Pv4CL1 down-regulated. RNA interference of Pv4CL1 reduced extractable 4CL activity by 80%, leading to a reduction in lignin content with decreased guaiacyl unit composition. The transgenic plants had …
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A Role for Inositol Pyrophosphates in Arabidopsis Defense Against Herbivorous Insects
… and jasmonate-(JA)-regulated plant defense. Transgenic plants that overexpress inositol tetrakisphosphate 1-kinase (ITPK1) and the kinase domain of the dual domain diphosphoinositol pentakisphosphate kinase 2 (VIP2KD) have been previously studied to display uniquely elevated PP-InsPs. Here it …
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The Role of Acyl Carrier Protein Isoforms in Determining Plant Lipid Content and Composition
… Multiple isoforms of ACP are found in all higher plants. These isoforms differ in expression in various tissues and organs, and they also differ in the timing and regulation of expression during plant growth and development. The reason for the diverse number of ACP isoforms in plants is not …
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Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants
… p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a …
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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum
… to express fully processed porcine relaxin in transgenic tobacco plants, as an alternative to current methods of producing relaxin. Two recombinant relaxin genes were constructed that contained the patatin signal peptide cDNA fused in frame to prorelaxin cDNA, which was codon-optimized for …
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Tissue culture and transformation of introducing genes useful for pest management in rice
… of effective defense mechanisms for protecting plants against fungal pathogens. This research was conducted to co-transform the ¦Â-1, 3-glucanase, chitinase and bar genes into the rice variety Taipei 309 using the hpt gene for resistance to hygromycin B as a selective marker. Transformed calli …
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Coat Protein -Mediated Resistance of Apple Mosaic Virus in Nicotiana Benthamiana
The detection of protein accumulation in transgenic plants carrying the full-length ApMV-CP and conferring resistance to ApMV suggested that transgenic coat protein accumulation was involved in eliciting the ApMV resistance response. Protein accumulation of lower-truncated ApMV-CP did not induce …
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Role of Subcellular Differentiation in Plant Disease Resistance
… in the isoprenoid pathway. In solanaceous plants, one class of endproducts of the pathway are sesquiterpenoid phytoalexins, antibiotic compounds produced by plants in response to pathogens. We are interested in the role of the defense-inducible isoforms of HMGR in phytoalexin production and …
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Expression of Human Protein C in Transgenic Tobacco
… g-carboxylation, and N-linked glycosylation. Plants have the eukaryotic protein modifying mechanisms required for many human proteins and may provide a safe, cost-effective system for producing hPC on a large-scale basis. Tobacco (Nicotiana tabacum L.) is particularly well suited for use as a …
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Toward developing pheromone emitting trap crops: Metabolic engineering of an aggregation pheromone for enhanced attraction of Phyllotreta cruciferae
… of interest. Generating pheromone-emitting trap plants may allow for a continuing and highly-specific attraction of insect pests without repeated and costly application of synthetic pheromones. These trap plants may also be used to develop area-wide pest management strategies. As a …
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