Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Transgenic Mouse Line"”.
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Analysis of Circadian Rhythm Using a Novel SCN-Specific Cre Transgenic Mouse Line
… test this hypothesis, a novel SCN-specific Cre transgenic mouse line, named NMS-Cre, was developed by inserting a bicistronic Cre expression cassette at the 3’-untranslated region of the Neuromedin S (NMS) gene. By crossing NMS-Cre line to a lox-STOP-lox diphtheria toxin receptor line, …
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A novel genome-wide approach to identify in vivo targets of Notch signalling
… of downstream effectors. I have established transgenic mice carrying Biotin Acceptor Peptide (BAP)- tagged versions of Notch1. The tagged protein is fully functional and is biotinylated after crossing to mice expressing the biotinylase from E. coli. Biotinylation was confirmed in a range of …
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Upper-respiratory infection triggers experimental autoimmune encephalomyelitis onset in autoimmune prone t-cell receptor transgenic mice
… pathology in an autoimmune-prone T cell receptor transgenic mouse line. To test this hypothesis we infected 2D2 mice and monitored for symptoms of inflammatory demyelination (EAE). Clinical and histological EAE was observed in ~29.2% of infected 2D2 mice which closely resembles the incidence of …
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The GluK4 Kainate Receptor Subunit Regulates Mood, Memory and Excitotoxic Neurodegeneration
… of GluK4, I generated a global GluK4 knockout mouse line by crossing a germline-expressed cre transgenic mouse line with a GluK4-floxed mouse line in which exon 16 of GRIK4, the gene encoding GluK4, was flanked by lox-P sites (floxed). Thorough characterization of GluK4 expression in the …
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Developmental And Signaling Mechanisms Of Touch Neurons
… conditions. We describe the development of a transgenic mouse line, Aβ-ReaChR, in order to specifically activate the Aβ class of LTMRs using optogenetic stimulation using three different mouse lines, Split-Cre, Advillin-FlpO, and Rosa-ReaChR. We then discuss how these tools may be applied to …
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THE ROLE OF THE TRANSCRIPTION FACTOR DEAF-1 IN PROSTATE FUNCTION
… with decreased apoptotic response in knockout mouse embryo fibroblasts. Heterozygous Deaf-1 males develop prostate inflammation and prostate T-Cell lymphomas suggesting DEAF-1 haploinsuffciency produces a genetic defect in prostate function. The Deaf-1 knockout mice also develop lung and liver …
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Converging roles of neurodevelopment and Wnt signaling in neuropsychiatric disorders
… World Health Organization. These findings underline the vast burden caused by neuropsychiatric disorders on patients. However, effective treatments do not exist for many of the neuropsychiatric disorders mostly due to lack of understanding of disease pathology. Evidence from whole genome …
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Neurobiological mechanisms underlying episodic memory retrieval
… process. Second, we examined memory engrams in transgenic mouse models of early Alzheimer's disease, which required the development of a novel two-virus approach. We demonstrated that optical induction of long-term potentiation at input synapses on engram cells restored both spine density and …
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Activation of Early Neural Progenitors Is Required for Traumatic Brain Injury-Induced Hippocampal Neurogenesis
… I have characterized a previously generated transgenic mouse line that has rtTA-IRES-eGFP expression under the control of a nestin promoter and also contains a neural progenitor-specific regulatory element. By using this line, I have demonstrated that eGFP-expressing cells represent early …
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Identification and characterization of epithelial progenitor niches in skin
… populations, however, can also contribute to lineages beyond their local niche in response to wounding or other traumas. The goal of this thesis was to identify additional epithelial progenitor niches in the skin and characterize them. I focused on identifying and investigating the role of …
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Cadmium exposure impairs adult neurogenesis, cognition, and olfactory memory in mice
… using caMEK5 mice, a gain-of-function knock-in transgenic mouse line that allows inducible and conditional activation of adult neurogenesis, I found that enhancing adult neurogenesis by genetic manipulation after Cd exposure can rescue mice from Cd-induced impairments of cognition and olfactory …
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An inducible, conditional and targeted B cell ablation mouse model for studying B cell functionality in the pathogenesis of human diseases
… (DT) receptor combined with their respective transgenic strategies. The ablation using HSVtk transgenic mice eliminates dividing cells, but does not affect non-dividing cells. In addition, because of its extremely high potency (a single molecule of DT-A, the active cleavage product of DT is …
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Effects of the OTX2ECR2 enhancer & PROX1 overexpression in murine retina development
… gene in the developing retina of the mouse. OTX2 is a homeobox transcription factor important for the development of photoreceptors and bipolar cells. If OTX2 is knocked out, photoreceptors and bipolar cells fail to differentiate. In order for OTX2 to be activated in certain retinal …
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A Molecular Dissection of the Ventromedial Hypothalamic Nucleus
… neuronal populations, we created LBH2-GFP BAC transgenic mouse line expressing GFP under the control of the LBH2 promoter. These mice were validated for correct expression of the transgene and examined for overlap with other populations of interest in the VMH, including estrogen receptor-alpha …
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Elucidating the role of LARP6 in cardiovascular remodeling
… manipulated the function of LARP6 in two mouse models and exposed them to clinically relevant cardiac stressors. For the first aim, we utilized the 5'SL mutant mice which possess a mutation within the 5'SL of collagen I transcripts that prevent LARP6 from binding and is reported to …
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Analyse transgener Mauslinien mit zelltypspezifischer Expression fluoreszenter Proteine als Modelle für akute Hirntraumata
In der vorliegenden Arbeit wurden zwei Mauslinien (PRDA und PRDB), in denen das rote fluoreszente Protein DsRed unter der Kontrolle des PLP-Promotors exprimiert wurde, charakterisiert. Beide Linien zeigten ein in jeder Hinsicht ähnliches Expressionsmuster. DsRed wurde vom zweiten Postnataltag an im …