Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Transgenic Animals"”.
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Evaluation of techniques for the production of transgenic animals
… By comparison, the integration frequency for transgenic mice made using the same DNA construct was 22%. After 5 days of in vitro culture, the injected construct was detected in 83% of arrested 1-cell, 85% of arrested 2-cell, and 85% of fragmented embryos. Only 28% of zygotes cultured after …
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Improvement of expression of recombinant human protein C in the milk of transgenic animals using a novel transgene construct
… specific transcript and protein levels in the transgenic animals studied suggest that the rates of translation initiation may be different for the mRNAs of each of the transgenes studied.
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Developmental Resilience to Dopaminergic Vulnerability Associated With Human Immunodeficiency Virus
… may be mounted by the CNS. Periadolescent HIV-1 transgenic rats were confronted with a dopamine challenge, following which mesencephalic tissues were assayed to determine changes in the expression of biomarkers vital to dopamine neurotransmission. In assaying inherent differences between control …
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Liposome-Mediated DNA Transfer
The production of transgenic animals carrying a single transgene is very expensive. Two novel methods to deliver transgenes to animals were examined. The first method involved the use of high pressure to increase transfection efficiency in cell lines as a potential way to produce transgenics …
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Analysis of a Proposed Cell Binding Site on Caenorhabditis Elegans Collagen Type Iv
… Microinjection techniques were used to produce transgenic animals and generate worm strains with a mutation on the alpha 1 chain in the putative D-D-R site. These strains were produced in a null mutant background (no alpha 1 chain produced) and in a wild type background. Control strains were …
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Clinical and pathological characterization of a novel transgenic animal model of diabetes mellitus expressing a dominant negative glucose-dependent insulinotropic polypeptide receptor (GIPR dn)
… and pathological characterization of a novel transgenic animal model of diabetes mellitus expressing a dominant negative glucose-dependent insulinotropic polypeptide receptor (GIPR dn) Gastrointestinal hormones like glucose-dependent insulinotropic polypeptide have recently been shown to be …
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Tetracycline-dependent gene expression in cholinergic neurons of transgenic mice : a tool to study Neuregulin-1 function in vivo
… BAC construct was used for the generation of transgenic animals by oocyte injection. The ChAT-tTA2S transgenic mice were subsequently characterized by in situ hybridization and by mating them to reporter mice carrying a tTA-inducible ?-galactosidase gene. In two independent transgenic founder …
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In Vivo Investigation of Protein Interactions in Caenorhabditis Elegans by Forster Resonance Energy Transfer Microscopy
… were investigated by FRET microscopy in living transgenic animals expressing CFP- and YFP-fusion proteins. Quantitative FRET microscopy in living C. elegans was most complicated by large autofluorescence contributions to the measured signals. Heterogeneous absolute and relative fusion protein …
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The molecular and behavioral characterization of cav1.3 over-expressing mice
… lead to agerelated cognitive decline. A line of transgenic mice that over-express the LVGCC Ca<sub>V</sub>1.3 in the forebrain was developed in order to investigate the relative contribution of this change to age-related cognitive decline. These animals were also characterized on both on a …
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Regulation of Brain-Derived Neurotrophic Factor in the Adult Mouse Brain
… exons in the adult mouse brain; use of transgenic animals to define BDNF promoter regions; and use of comparative genomics to identify evolutionarily conserved regions of BDNF. The in situ hybridization study suggests that the four distinct BDNF promoters are differentially regulated and …
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Controlled ablation of rod photoreceptors in transgenic Xenopus laevis
… of rod cell death in the retina, we generated transgenic X. laevis that expressed a novel form of caspase-9, with binding domains specific to the compound AP20187. We treated these transgenic animals with AP20187 and caused rod cell death by apoptosis in tadpoles and post metamorphic animals. …
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Discovery of a Novel Signaling Circuit Coordinating Drosophila Metabolic Status and Apoptosis
… <italic>Drosophila</italic> S2 (S2) cells and transgenic animals to untangle the influence of metabolic status on fly apoptosis.</p><p>We first demonstrate a novel link between <italic>Drosophila</italic> apoptosis and metabolism by showing that cellular NADPH levels modulate the fly initiator …
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Modification of the Bovine Genome for the Large-Scale Production of Human Serum Albumin
… One solution to the problem is the use of transgenic animals, such as cattle, as living bioreactors, enabling large-scale production of recombinant HSA (rHSA) in a cost-effective manner. Cattle are capable of producing large amounts of milk that can potentially yield large quantities of a …
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR IN RETROGRADE TRANSPORT ALONG MOTORNEURON AXONS
… gene recombination technology our lab generated transgenic mice in which the BDNF gene was knocked out of skeletal muscle. We hypothesized that skeletal muscle-BDNF is required for normal retrograde transport of proteins and mitochondria along gastrocnemius-associated motorneuron axons. We first …
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Characterization of C. Elegans Pat-9 and Frg-1, Genes Critical for Body Wall Muscle Development
… between the nucleus and cytoplasm, and 25% of transgenic animals overexpressing FRG-1 showed disruptions in body wall musculature, including smaller, misaligned, missing and disconnected muscle cells. FRG-1 is highly evolutionarily conserved suggesting human FRG1 may have the similar expression …
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Production and Secretion of Recombinant Human Fibrinogen by the Transgenic Murine Mammary Gland
The mammary gland of lactating transgenic animals has several advantages for production of heterologous proteins including a high cell density that results in high concentrations of secreted protein and the ability to perform several types of post-translational modifications. Transgenes were …
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Role of Il-17/Il-17Ra Signaling In The Pancreatic Tumor Microenvironment
… tumor promotion remains unknown. We generated a transgenic mouse model with pancreatic KrasG12D activation and selective deletion of IL-17RA in the epithelial vs. immune component using either genetic manipulation or bone marrow transplantation. Deletion of IL17RA from the pancreatic epithelial …
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Role of MOA-1, an Anti-phosphatase, and APL-1, an APP-related Protein, in Morphogenesis and Mating Behavior in Caenorhabditis elegans
… mating behavior of yn5 mutants, <em>moa-1</em> transgenic animals, and other mutants regulating male tail development. Strains were characterized for morphology and mating behavior without knowledge of the genotype of the animals. Understanding male tail development may provide insights into …
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Effects of Early Alcohol Exposure on Ocular Dominance Plasticity
… of ODP. Ethanol exposed and saline control animals were MD for 5, 7 or 10 days during the critical period of the visual cortex. Here we saw that although saline animals exhibited a normal depression of contralateral eye responses and a potentiation of ipsilateral eye responses, ethanol …
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