Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Transgenesis"”.
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Large-scale transgenesis and nerve regeneration in C. elegans
… for laser neurosurgery screening and transgenesis in C. elegans. Discovery of molecular mechanisms and chemical compounds that enhance neuronal regeneration can lead to development of therapeutics to combat nervous system injuries and neurodegenerative diseases. By combining …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… generation of transgenic medaka fish. General transgenesis including transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first time efficient enhancer trapping in …
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Universal robot for automated microinjection with applications in transgenesis and cryopreservation
… microinjection into zebrafish embryos. We used transgenesis studies to compare microinjection efficiency to manual microinjection utilizing optimum settings for automated microinjection. Further, we demonstrated that robotic microinjection of cryoprotective agents in zebrafish embryos …
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Towards unravelling the immunomodulatory mechanisms of S. mansoni & L. major parasites in a mouse model using transgenesis
Schistosoma mansoni and Leishmania major parasites use immunomodulatory strategies to evade the host’s immune response and little is known about the mechanisms by which these two parasites modulate host immune responses. As a result, these parasites develop different subversion mechanisms to escape …
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A lentiviral system for RNAi transgenesis and the Ena/VASP triple-knockout defines neuronal and non-neuronal functions in mouse development
Mammalian development extends and exploits signaling pathways that function exclusively in axon guidance in lower organisms. This emerging paradigm employs complex expression patterns of expanded protein families to achieve the complexity and specificity required in mammalian development. For …
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Studies on improvement of Chinese wheat cultivars for resistance against an endemic virus using transgenesis and virus-wheat interactions at the molecular and ultrastructural levels
… to Soil-borne wheat mosaic virus (SBWMV) by transgenesis, and an examination of virus movement by studying the complementation of movement proteins (MPs) from Tobacco mosaic virus (TMV) and SBWMV using a TMV-based virus vector.<br/><br/>A number of SBWMV coding sequences, including the …
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Development of transgenic Ambystoma mexicanum (axolotl) to study cell fate during development and regeneration
The establishment of transgenesisi in axolotls is crucial for studying development and regeneration, as it would allow for long-term fate tracing as well as gene expression analysis, therefore we were interested in both obtaining animals expresing the transgene with little mosaicism in F0 …
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The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes
… five analyses. Southern Analysis detected 2.8% transgenesis. Cytoplasmic injection of linear CMV-EGFP (625 µg/ml in water) was 3.7% transgenic. Pronuclear injections produced 7.9% transgenesis. This research identified a range of reversible electroporation that could easily be verified <i>in …
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Acf7 is a Hair-Bundle Antecedent, Positioned to Integrate Cuticular Plate Actin and Somatic Tubulin
… cell development and function. Through zebrafish transgenesis and indirect labeling of mouse hair cells we show that ACF7 is positioned to integrate the actin and microtubule networks in hair cells. In addition, electron micrographs demonstrate microtubules inserting into the cuticular plate, the …
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Development of broadly applicable transgenic tools for the transposon mutagenesis of the red flour beetle, Tribolium castaneum
… a universal 3xP3 promoter revolutionized insect transgenesis. It overcame the limitations of the germ-line transformation to be restricted to the model organism Drosophila melanogaster. In combination with discernable fluorescent markers, multi-component systems, such as transposon-based …
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Spatial Expression Patterns of theSnmp Gene Family In Drosophila Melanogaster
… for SNMP1 as well as SNMP2. Via the GAL4-UAS transgenesis system in tandem with immunohistochemical methods, we show that SNMP1 expresses in neural and support cells of the antennae as is consistent with the aforementioned studies. However, it is also shown that SNMP1 expression is not limited …
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Molecular and Functional Characterization of Pyramidal Cell Types in the Frontal Cortex of Mouse Brain
… this dissertation presents evidence that BAC transgenesis can be applied to genetically access pyramidal cell types in the neocortex for molecular profiling as well as functional characterization using silencing viruses and conditional alleles. We also showed direct utility of TRAP data in …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
… for macromolecule delivery in transfection or transgenesis.
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Transgenic Tools in Ants and the Representation of Alarm Pheromones in the Ant Antennial Lobe
… ant Ooceraea biroi, we implemented piggyBac transgenesis for the first time in ants, and generated a toolkit of transgenic lines. These protocols and transgenic tools greatly expand the space of feasible experiments in ants, and make clonal raider ants the most experimentally tractable model …
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An ancestral regulatory mechanism underlies Hoxd gene expression in both developing genitals and digits
… embryonic structure. We used lentivirus-mediated transgenesis to evaluate the enhancer activity of the identified regulatory elements. While some sequences were able to drive a reporter gene in both digits and genitalia, others were specific for either one of these two structures. We identified …
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Using Drosophila to study the contribution of human kinases to tau toxicity
… its expression, I used a novel method of transgenesis to insert the human tau transgene in a reproducible position within the fly genome. Using this system, tau isoforms differing for the presence of two N-terminal domains were shown to generate the same level of toxicity in the fly eye. …
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Modelli murini transgenici di malattie da prioni per lo studio del ruolo fisiopatologico della proteina prionica.
… eggs from C57BL/6J X CBA/J parental mice (random transgenesis), we identified Tg mice which were backcrossed to PrP knockout (C57BL/6J/Prnp0/0) mice. We have obtained vary Tg founders: carrying D177N/V128 or D177/M128 PrP. The Tg copy number of the founders was determined by quantitative PCR, and …
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Oncogenic mutation of GNAQ/11 disrupts melanocyte biology in a Zebrafish model of uveal melanoma
… that we have successfully used Tol-mediated transgenesis to develop a zebrafish model of UM driven by melanocyte specific expression of human oncogenic GNAQ/11Q 209L alleles. In cooperation with tp53M214K/M214K mutation, these GNAQ/11Q 209L transgenic zebrafish develop both uveal and …
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The Role of Insulin-like Growth Factor-I and IGF-binding Proteins in Mammary Gland Development
… into milk of transgenic mice. Regardless of transgenesis, a shift in the milk IGFBP profile from predominantly IGFBP-3 to a lower molecular weight IGFBP occurred between d 8 and d 12 of lactation. The altered composition of milk from transgenic line 29 dams reduced by 27% the average daily …
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A NOVEL METHOD TO INTERFERE WITH GENE EXPRESSION IN MICE
… Despite the general low efficiency of transgenesis the down regulation of HNF4gamma gene in one F1 line (A-I) reached 50% in the gut and 80% in pancreas proving that this targeted knockdown approach is working in living animals.
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