Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 381 for “"Transferase"”.
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Investigation of the yeast oligosaccharyl transferase
Oligosaccharyl transferase catalyzes the first commited step in N-linked glycosylation. The enzyme complex from Saccharomyces cerevisiae includes nine membrane bound proteins located in the lipid bilayer of the endoplasmic reticulum, five of these proteins are essential for cell viability. The …
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Characterization of Yeast 18S rRNA Dimethyl Transferase, Dim1p
… ubiquitously carried out by the Dim1/KsgA methyltransferase family. Although dimethylation and KsgA are dispensable for survival in bacteria, the eukaryotic enzyme Dim1 is essential because of its requirement in the early pre-rRNA processing events. Similarly, few other members of the family have …
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Understanding tetratricopeptide repeats of <i>O</i>-GlcNAc transferase
… of enzymes modulate this modification: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Intriguingly, OGT has also recently been shown to have a secondary function, catalysing the proteolysis of the transcriptional regulator protein host cell factor-1 (HCF-1). OGT contains an N-terminal …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… by two antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto protein substrates and the O-GlcNAc hydrolase (OGA), which removes it. Mass spectrometric analyses have shown that O-GlcNAc can be found on proteins involved in nearly all biological processes, including …
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Methyl Chloride Transferase: Isolation, characterization,cDNA Cloning, and Expression
The genomic DNA of methyl chloride transferase of Batis maritima was also characterized and is composed of 8 exons and 7 introns.
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Studies towards the in vivo inhibition of oligosaccharyl transferase
… This process is catalyzed by oligosaccharyl transferase (OT), which is a membrane-associated enzyme found in the lumen of the endoplasmic reticulum (ER). A variety of inhibitors that bind tightly to OT in vitro, with K[sub]i s as low as 10 nM, have been synthesized. The development of …
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Investigation of the structure requirements for oligosaccharyl transferase function
… and diseases (Varki, 1993). The oligosaccharyl transferase (OTase), the principle enzyme responsible for catalysis of N-linked glycosylation, facilitates the transfer of a preassembled oligosaccharide from a polyprenyl-lipid donor to the side-chain nitrogen of asparagine acceptors. In a majority …
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Identification and Characterization of A Zebrafish Glutathione S-Transferase Pi
Glutathione S-transferases (GSTs) are a major group of Phase II enzymes involved in the detoxification of certain endogenous compounds such as reactive oxygen species (ROS) generated during metabolism, as well as in the detoxification of xenobiotics including drugs. In recent years, the zebrafish …
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Characterisation of the cell-surface localised palmitoyl acyl transferase, DHHC5
… and disease pathways. DHHC palmitoyl acyl transferase (DHHC-PAT) enzymes are responsible for catalysing the addition of acyl groups onto cysteine residues and there are 23 human DHHC-PAT isoforms. All isoforms share a conserved DHHC catalytic motif and they display heterogeneity in their N- …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… process is catalysed by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), that add and remove the sugar moiety, respectively. Ogt gene knock-out is embryonic lethal in a range of animal models, hampering the study of the biological role of O-GlcNAc. O-GlcNAcylation of nuclear and …
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Development of transition state analogues targeting chitinases and oligosaccharyl transferase
Oligosaccharyl transferase (OT) plays a central role in the biosynthesis of asparagine-linked glycoproteins in eukaryotic systems. The glycosylation step catalyzed by OT involves the co-translational transfer of a tetradecasaccharide from a dolichyl-pyrophosphate carrier to an asparagine side-chain …
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Purification and characterization of methyl chloride transferase: A novel halogenating enzyme
… methionine (SAM) as a methyl donor in a methyl transferase reaction in which chloride, bromide and iodide are all methyl acceptors.
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Oxygen Atom Transfer Reactions Catalyzed by Molybdenum and Tungsten Oxo Transferase Mimics
The ability to mimic enzymatic oxidation reactions with synthetic catalysts is essential for advancing selective and sustainable chemical transformations. Nature employs molybdenum and tungsten enzymes to mediate oxygen atom transfer (OAT) reactions, which are critical in biological redox …
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Polymorphismen der Glutathion-S-Transferase A1 in Zusammenhang mit malignen hämatologischen Erkrankungen
… zu sein. Da die Familie der Glutathion-S-Transferasen innerhalb des Phase-II-Stoffwechsels eine zentrale Rolle in der Entgiftung spielen, waren und sind auch ihre Polymorphismen äußerst forschungsrelevant.
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Apolipoprotein E Activation and Phosphatidylcholine Substrate Requirements of Lecithin Cholesterol Acyl Transferase
… activation of lecithin cholesterol acyl transferase (LCAT) by human apolipoprotein E (apo E) was investigated by incorporating apo E into discoidal complexes of egg phosphatidylcholine (egg-PC) and cholesterol by the cholate dialysis method. These complexes were systematically compared to …
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