Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Transcriptomic techniques"”.
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Mechanisms of DNA double strand break-mediated neurotoxicity in neurodegenerative disease
… using single-nucleus, bulk, and spatial transcriptomic techniques. DSB-bearing neurons enter a late-stage DNA damage response marked by NFκB-activated senescent and antiviral immune pathways. In humans, Alzheimer’s disease pathology is significantly associated with immune activation in …
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Dry and back again: characterization of desiccation-associated differentiation of leaf tissues in Craterostigma pumilum Hochst
… tissues. In this thesis, physiological and transcriptomic techniques were used to characterize such a phenomenon in the homoiochlorophyllous dicot resurrection plant Craterostigma pumilum. Detailed phenotypic observation and pulse-amplitude-modulation fluorometry were used to identify the …
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Vascular smooth muscle cell heterogeneity and plasticity in models of cardiovascular disease
… and observed functionality, the single cell transcriptomic techniques Smart-seq2 and the Chromium 10X system were optimized for use on VSMCs. The work within this thesis suggests that extensive proliferation of a low proportion of highly plastic VSMCs results in the observed VSMC accumulation …
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Exploring INSL5 and RXFP4 in the gut-brain axis and their potential role in feeding behaviour
… model combined with immunohistochemistry and transcriptomic techniques, we identified Rxfp4 expression in enterochromaffin, L and tuft cells in the colon, in neurons of the dorsal root ganglia (DRG) and nodose ganglia (NG), and in multiple brain regions associated with feeding. By combining …
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Tracing RNA biography: in situ transcriptome profiling by novel spatial omics technologies
… cells. Presently, the state-of-the-art spatial transcriptomic techniques offer only static snapshots of RNA expression, falling short of capturing RNA dynamics and their controlled translation within subcellular domains. Therefore, our driving question is whether the spatial regulation of …
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Ecotoxicogenomic New Approach Methods for Screening Development in Danio rerio
… modeling, in vitro assays, and omic techniques, which align with the 3R principles — Replacement, Reduction, and Refinement of animal experiments. Among these NAMs, transcriptome analysis is highlighted for its potential to provide comprehensive and mechanistic insights into chemicals …