Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 36 for “"Transcriptomic profiles"”.
-
The Microsatellite Instability High Endometrial Tumor Microenvironment and Identifying Predictive Biomarkers of Response to Pembrolizumab
… in the tumor microenvironment (TME) and identify transcriptomic signatures associated with response/resistance to pembrolizumab. Methods: Archival tumor samples from MSI-H endometrial cancer patients treated at MD Anderson Cancer Center were obtained. Tissue samples originating from patients who …
-
The Molecular Regulation of Wiring Specificity in the Serotonin System Across Key Postnatal Stages
… cortical and subcortical subsystems with varying transcriptomic profiles. These genetically distinct, projection specific subsystems require appropriate signals during axon innervation. However, the molecular mechanisms behind how serotonin neurons specifically innervate the correct target region …
-
Characterization of DNA methylation and gene expression profiles in post-traumatic stress disorder
… DNA methylation (DNAm) and gene expression profiles, exhibit tissue specificity and are comprised of two dynamic components: 1) profiles reflecting cellular composition and 2) alterations in DNAm levels at CpG sites and transcriptional activity. In this work, I examine DNA methylation and …
-
Design and Development of Oligonucleotide Microarrays and their Application in Diagnostic and Prognostic Estimation of Human Gliomas
… gene expression assay employed to study the transcriptomic profiles of biological tissues. These devices are increasingly being used to study many aspects of gene regulation, and there is growing interest in the biotechnology and pharmaceutical industries for developing such devices in …
-
Deep representation learning for cancer transcriptomics: towards robust models for heterogeneous data
… treatment. Molecular data, including genomic and transcriptomic profiles, are used to train these ML systems. But current models are very sensitive to distributional shifts in the underlying training data, which arise from variations in collection, preservation and processing methods. This …
-
Mapping and modeling macrophages in tuberculosis
… broadly. We first characterize the single-cell transcriptomic profiles of macrophages within tuberculosis granulomas from non-human primates. We introduce and apply a framework for identifying possible signaling cues contributing to these states using ex vivo-generated macrophage models. To …
-
SYSTEMS BIOLOGY APPROACHES TO DECIPHERING COMPLEX IMMUNE RESPONSES
… stimuli. We created a dictionary of single-cell transcriptomic profiles of individual responses to 86 cytokines and 5 vaccine adjuvants in over 20 cell populations, representing one of the most comprehensive analyses of cellular responses to immune stimuli to date. Based on the dictionary, we …
-
Epigenetic & transcriptomic alterations associated with childhood maltreatment, major depressive disorder, and post-traumatic stress disorder
… In Chapter 4, the genome-scale blood-based transcriptomic profiles of childhood maltreatment and PTSD are characterized and compared for overlap. Chapter 5 characterizes the genome-scale DNA methylation profiles associated with MDD in post-mortem brain. Results reveal that, despite clear …
-
Transcriptomic studies of protein homeostasis perturbations in neurodegenerative disorders
… Our analysis was initially carried out on transcriptomic profiles of bulk tissue data, and was subsequently tailored to single-cell RNA sequencing datasets, in order to verify how working at different resolutions would affect our findings. Dimensionality reduction and clustering techniques …
-
Transcriptomic profiling of macrophage polarization
… most popularly used platforms for single-cell transcriptomics. I then focus on my work characterizing macrophage polarization and repolarization in vitro, where I performed deep transcriptomic profiling at high temporal resolution as macrophages were polarized with cytokines that drive them …
-
Transcriptomics-based study reveals a novel link between arginine methylation and phosphate regulation in Saccharomyces cerevisiae
… of Hmt1. RNA-seq was used to compare the transcriptomic profiles of wild type versus Hmt1-null cells. In mid-log exponential growth, we found dysregulation of phosphate metabolism and polyphosphate accumulation in the Hmt1-null strain. Acid phosphatases (PH05, PH011 and PH012), phosphate …
-
Causal Representation Learning for Predicting Genetic Perturbation Effects on Single Cells
… technique, enabling high-resolution profiling of transcriptomic responses to genetic perturbations at the single-cell level. Such insights have profound implications for functional genomics and the identification of therapeutic targets. This thesis investigates the efficacy of mechanistic …
-
INFERRING TUMOR PROPERTIES FROM GENE EXPRESSION DATA
The wide availability of transcriptomics data, along with the advancement of experimental technologies, computational tools, and statistical methods like machine learning (ML), has significantly improved the use of transcriptome data in cancer research, benefiting both diagnostic and prognostic …
-
Multi-Omics Analysis of 3D SKOV3 Ovarian Cancer Model Cell line Cultured on Novel Silicon-Titanium Diboride Micropatterned Substrate
… day seven, and disaggregation at day nine. The transcriptomic profiles of the SKOV3 cell lines were analyzed across the different time points to capture the dynamic, functional, and molecular signature changes in 3D microenvironment on the culture platform. The transcriptomics analysis …
-
Survival strategies of Mycobacterium avium subsp. paratuberculosis in a variety of microenvironments.
… speed of infection as well as host and pathogen transcriptomic profiles. Taken together, data generated from these studies will provide the basis for understanding MAP persistence and survival in diverse conditions. The mechanisms by which MAP establishes, disseminates and/or survives difficult …
-
Adaptive radiation and speciation in African weakly-electric fish
… genetic basis of EOD production by exploring the transcriptomic profiles of the electric organ and of its ancestral counterpart, the skeletal muscle, in the genus Campylomormyrus. After having established a set of reference transcriptomes using “Next-Generation Sequencing” (NGS) technologies, I …
-
Systems analysis of the CD4 T cell response induced by the novel subunit tuberculosis vaccine, H1:IC31
… novel vaccine candidate, H1:IC31, by measuring transcriptomic, phenotypic and functional attributes, and to determine the effects of latent infection on these responses. Firstly, we found that acquisition of M.tb infection did not alter the breadth and/or pattern of Ag85A/ B CD4 T cell epitopes …
-
MULTI-OMICS APPROACHES TO CHARACTERIZE PATIENT-DERIVED SPINAL CORD ORGANOIDS AND ASSESS NOVEL GENES ASSOCIATED WITH C9ORF72-AMYOTROPHIC LATERAL SCLEROSIS
… At the endpoint, SCOs underwent proteomic and transcriptomic analyses by performing nano-liquid chromatography–high-resolution mass spectrometry (nLC-HRMS), western blot (WB), qPCR, and single-cell RNA-sequencing (scRNA-seq). ICC and qPCR revealed the expression of neural, pan-neuronal, and MN …
-
Probing the role of histone acetylation in the evolution of pathogenicity in <i>Candida glabrata</i>
… strains also showed substantially different transcriptomic profiles to the wild-type yeast and in context with the literature, transcriptomic data suggests that these histone acetylation-related genes have functionally diverged in the <i>C. glabrata</i> lineage, in accordance with our in …
-
Computational reconstruction of mouse development using single cell transcriptomics
Single cell transcriptomics has significantly contributed to our understanding of cell types across species, organs and developmental processes. The rapid development of technologies, protocols and computational methods reflects a highly dynamic field over constant improvement. Particularly for …
Page 1 of 2