Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Transcriptome profiling"”.
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… allowed me to then perform spatially defined transcriptome profiling. Preserving the spatial coordinates of each sample processed for transcriptome analysis enabled me to integrate the transcriptome information of each sample individually into 3D embryo reconstructions. A collaborator applied …
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Olfactory Ensheathing Cell Development: A Transcriptome Profiling Approach
… Schwann cell differentiation, I took an unbiased transcriptome profiling approach. Taking advantage of Sox10 expression throughout both OEC and Schwann cell development, I used laser-capture microdissection on cryosections of mouse embryos carrying a Sox10:H2BVenus transgene, to isolate OEC …
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Long-read transcriptome profiling of germinal centre B cells
… the differentially spliced transcript variants. Transcriptome characterisation using ONT allowed us to detect differentially expressed transcripts during antigen-mediated activation of GC B cells in human and mouse. Moreover, identification of individual isoforms with Nexons revealed differential …
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Single-cell Transcriptome Profiling Reveals Hedgehog Critically Regulates Hepatocyte Resiliency
… in adult liver. Using single-cell transcriptomic profiling, we demonstrated that Hedgehog activity was spatially restricted to hepatocytes localized in the mid-lobular area. By manipulating Hedgehog activity in hepatocytes of intact mice and challenging them with an acute intraportal injection of …
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Enhanced transcriptome profiling and biomarker discovery using meta-analytical techniques
This is a comprehensive study of the application of meta-analytical techniques to gene expression data to detect differentially expressed genes across experiments and conditions, and as a means of classifying unknown samples. Three chapters were created to: (1) investigate three novel meta-analysis …
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Tracing RNA biography: in situ transcriptome profiling by novel spatial omics technologies
… resolved post-transcriptional regulations within transcriptomes, studying these events at the single-cell and single-molecule level. This advantage is important for mapping the complex network of transcriptional and post-transcriptional gene regulatory mechanisms inherent in cells and tissues. …
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Transcriptome profiling of infected chickens with newly emerged genotype VI I Newcastle disease virus strains
… tools and PPI network analysis. Transcriptomic profiling indicates that virulent NDV-GVII significantly downregulates immunologically regulated genes and innate immune regulating pathways including fMLP signalling in neutrophils, Fcy receptor-mediated phagocytosis in macrophages and monocytes, …
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Novel insights into megakaryopoiesis, thrombopoiesis and acute coronary thrombosis: transcriptome profiling of the haematopoietic stem cell, megakaryocyte and platelet
The aim of this project was to investigate the transcriptome of human haematopoietic stem cells (HSCs), megakaryocytes and platelets to gain insights into steady state and accelerated thrombopoiesis that occurs in states of haemostatic demand and in thrombosis by applying these findings to the …
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Novel methods in transcriptome analysis using RNA-seq
RNA-seq has proven to be a powerful technique for transcriptome profiling based on next-generation sequencing (NGS) technologies. Using RNA-seq, we want to solve two critical challenges: identifying Splice Junctions (SJs) and annotating gene fusion transcripts. Due to the limited read length of NGS …
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Comparison of different differential expression analysis tools for rna-seq data
… research, RNA-seq is a relatively new method for transcriptome profiling. It utilizes the next generation sequencing technology to provide huge amount information about the variety and abundance of RNA present in an organism of interest at a specific state and a given time. One of the most …
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Double-stranded RNA uptake in Caenorhabditis elegans
… RNA uptake. By combing small RNA and transcriptome profiling with developmental studies I identified a novel role for systemic RNAi in worm morphology. A detailed phenotypical analysis ruled out a nutritional role for dsRNA uptake, implying that environmental RNA uptake affects …
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A strategy to study pathway cross-talks of cells under repetitive exposure to stimuli
… standard techniques that are applied to do the transcriptome profiling of cells under repetitive stimuli and reveal gene regulatory networks. Therefore a well-established pipeline to analyze microarray data is of special help to investigate the underlying mechanism of priming effect. In this …
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Novel Regulators of Somatic Cell Reprogramming
… SI clones failed to reprogram at either stage. Transcriptome profiling by RNA-Seq revealed that SC clones acquire a competency gene expression signature late in the maturation phase. Functional RNAi screening of SC signature genes further identified regulators of transition to the stabilization …
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Epigenetic Regulation of Oligodendrocyte Development and Regeneration in the Central Nervous System
… Genome-occupancy analyses, coupled with transcriptome profiling, reveal that Chd7 interacts with Sox10 and targets the enhancers of key myelinogenic genes, and identify novel Chd7 targets including bone formation regulators Osterix/Sp7 and Creb3l2, which are also critical for …
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The role of miRNA-486-5p in hair growth and the hair follicle immune privilege
… study. In AA mouse model (C3H/HeJ), global miRNA profiling revealed 22 miRNAs with significant changes in their expression in AA affected skin. Amongst these miRNAs, miR-486-5p was dramatically decreased in alopecic skin in both humans and mice, in striking contrast to its prominent expression in …
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BREAST CANCER DURING PREGNANCY AS A SPECIAL TYPE OF EARLY-ONSET BREAST CANCER: INSIGHTS INTO THE TUMOR MICROENVIRONMENT AND IMMUNE TRANSCRIPTOME
… the tumor microenvironment (TME) and the immune transcriptome of PrBC to identify their differences from early-onset breast cancer (EOBC) in non-pregnant women. Eighty-three PrBC and 89 EOBC were selected and subjected to tumor-infiltrating lymphocytes (TILs) profiling and immunohistochemistry …
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Trim28 Interacts With Ezh2 to Regulate Its Activity
… domain) of EZH2 interacts with TRIM28. Transcriptome profiling and intersection of EZH2- and TRIM28-regulated genes indicated co-regulation of differentiation and morphogenesis pathways, as well as co-activation of the cytokine-cytokine receptor interaction pathway (CXCR4, CXCL12, EGFR, …
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