Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 137 for “"Transcriptome analysis"”.
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Transcriptome Analysis of Sea Lamprey Embryogenesis
… in the Eye Spot/Melanophore comparisons. This transcriptome analysis serves as a starting point for determining vital genes in sea lamprey embryogenesis and controlling the sea lamprey population.</p>
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Hippocampal Subfield Transcriptome Analysis in Schizophrenia Psychosis
… this doctoral dissertation examines the transcriptome of all three subfields, DG, CA3, and CA1 in human postmortem tissue of controls and individuals diagnosed with schizophrenia, using RNA-seq to identify additional psychosis-mediating molecular candidates and produce plausible targets …
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Novel methods in transcriptome analysis using RNA-seq
RNA-seq has proven to be a powerful technique for transcriptome profiling based on next-generation sequencing (NGS) technologies. Using RNA-seq, we want to solve two critical challenges: identifying Splice Junctions (SJs) and annotating gene fusion transcripts. Due to the limited read length of NGS …
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Preparation, characterisation and transcriptome analysis of RNA frm human vCJD brains.
… the molecular mechanisms involved. This requires analysis of human postmortem brain tissue however; the variability in RNA preparations from human brain material is a concern. A method for the isolation of RNA from vCJD brains which minimized infectivity and reduced Proteinase K resistant prion …
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Exploring flowering gene networks in soybean and arabidopsis through transcriptome analysis
… gene networks in soybean through an RNASeq based transcriptome analysis. Chapter One discusses the effect of three photoperiod treatments, short day (SD), long day (LD) and shift from 3 weeks LD to 1 week SD on six domesticated varieties of Glycine max; a reference variety, Williams 82, Clark and …
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Dynamics of soybean leaf hydraulic conductance and diurnal soybean transcriptome analysis
… embolized vessels. Finally, the diurnal soybean transcriptome was analyzed using RNA-seq to determine what genes, or groups of genes, may contribute to diurnal and circadian cycles in plant function. 30% of expressed soybean genes were found to be differentially expressed over the course of the …
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Understanding Plant Response to Stress Using Gene Model Quality Evaluation and Transcriptome Analysis
… strains was thus compared based on their transcriptome data, and a list of potential genes that were differentially expressed between them was identified. We also analyzed how much the quality of the transcriptome can be improved by using the transcriptome and genome of sunflower, a …
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Transcriptome analysis of the hypothalamic-pituitary-adrenal axis in the experimentally domesticated fox
… from the tame and aggressive lines, and variant analysis was performed on RNA reads from hypothalamus, anterior pituitary, and adrenal tissues from the same foxes. Pituitary analysis revealed expression differences in genes related to exocytosis and cellular signaling; adrenals analysis …
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Enrichment and Transcriptome Analysis of CD4+ T Cell Clones Harboring Intact HIV-1 Proviruses
… from uninfected CD4+ T cells, ex vivo analysis on a single cell level has not been feasible. In the first part of this thesis, a method for the enrichment of clonally expanded cells harboring an intact HIV-1 provirus that does not require latency reversal is presented. The enrichment is …
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Investigation of miRNAs Expression in a Citron-Kinase Mutant Model of Microcephaly
… approaches were utilized to analyze transcriptome analysis data comparing expression between the wt and Cit-K<em><sup>fh/fh</sup>.</em> Evaluation of miRNA expression between neural progenitor cells (NPCs) and cells grown in mitogen free media with BDNF+ (MFBCs) revealed diminished …
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Computational Analysis of Gene Expression Regulation from Cross Species Comparison to Single Cell Resolution
… of pathogens. Nowadays, a tremendous amount of transcriptome data sets are available from diverse species. This trend enables us to perform comparative transcriptome analysis that identifies conserved or diverged gene expression responses across species using transcriptome data. The goal of this …
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Functional Roles of the Drosophila gypsy Insulator Components
… both pds5 and CP190 genes, which is ready for transcriptome analysis. Reduction was observed in KC and 167 BG3 cells, but not significant. Future work is needed to test transfection efficiency in the other two cell lines and finally move to the transcriptome analysis.
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Bioinformatics in Traditional Chinese Medicine (TCM): Potential anti-cancer mechanisms of Compound Kushen Injection (CKI)
… still unclear. In this thesis, I first applied transcriptome analysis and bioinformatics methods as part of a pipeline to investigate interactions between CKI and chemotherapy drugs. With this pipeline, the mechanisms for the opposing effects of CKI combined with doxorubicin compared to …
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A Systems Approach to Studying the Differentiation of Stem Cells Towards Hepatocytes
… differentiation and cell growth. Using transcriptome analysis and mass cytometry, we showed how the population of cells changed over time on both the transcript and protein level. Both analyses revealed that with the new expansion stage, we obtained a higher quantity of hepatocytes …
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Senescence of Native Perennial Warm Season Grasses Senescence Associated Switchgrass Transcriptome
… to occur rapidly between mid to late September. Transcriptome analysis on early and late senescencing switchgrass cultivars reveals upregulation of starch metabolism, light reactions, Calvin-Benson Cycle, and anthocyanin synthesis in late senescencing switchgrass. Morphological variations between …
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Study of phenotypic and gene expression response to bacterial challenge
… Sequencing platforms, the scale of transcriptome analysis can be greatly increased. This study aims to address these previous limitations, characterizing behavioral and gene-expression responses to BCG-induced inflammation.
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Identifying genes that confer ethanol tolerance in Saccharomyces cerevisiae
… W303, via adaptive evolution (Stanley 2008). Transcriptome analysis of the resultant ET mutants found that expression levels of hundreds of genes were altered, relative to the parent under ethanol-stress conditions. The primary objective of this thesis was to identify genes that confer ethanol …
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The Role of Ifitm3 In The Immune Response of Brca-Deficient High Grade Serous Ovarian Carcinoma
… immune mechanism in these tumors, a preliminary transcriptome analysis was performed on a set of microdissected HGSOC tumor specimens with BRCA1-mutation, BRCA2-mutation, or WT. This demonstrated an upregulation of IFITM3, an essential gene in modulating immune function. Based on these findings, …
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Evolution and Genetics of Floral Color Polymorphisms in Clarkia gracilis ssp. sonomensis and Erythronium umbilicatum
… by an R2R3-MYB transcription factor using transcriptome analysis, gene expression assays and cosegregation examination. I also found that petal pigmentation requires at least four R2R3-MYB genes, each gene exhibiting a spatiotemporal expression pattern that is different from each other, and …
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Comparative genome analysis of human bifidobacteria with commercial potential
… subsp. longum genomes and subsequent comparative analysis with publicly available B. longum subsp. longum, B. longum subsp. infantis and B. longum subsp. suis genomes (Chapter 2). The acquired data revealed a closed pan-genome for this bifidobacterial species and furthermore facilitated the …
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