Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Transcriptional silencing"”.
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Molecular mechanism of KAP1-dependent transcriptional silencing
… KAP1 induces the assembly of an epigenetic silencing complex, with chromatin remodeling activities that repress transcription of the targeted retrotransposon and adjacent genes. To understand the molecular basis of KAP1-dependent transcriptional regulation I determined the crystal structure …
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Mechanisms of piRNA biogenesis and co-transcriptional silencing of transposable elements in Drosophila
… recognise active transposons and instruct their silencing. Ultimately, piRNAs trigger epigenetic silencing of transposon loci. The work in this thesis investigates the molecular mechanisms by which piRNAs are produced from the correct substrates and how piRNAs can induce silencing of target loci …
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Mechanisms of piRNA-guided co-transcriptional gene silencing
… transcripts where it regulates the formation of transcriptionally repressive chromatin states. As well as numerous chromatin modifying proteins, this mechanism depends on the Panx-induced co-transcriptional silencing (PICTS) complex, which contains Panx, Nxf2 and Nxt1. PICTS operates at the …
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CROSSTALK BETWEEN PRC1 AND PRC2 IN INTESTINAL LINEAGE COMMITMENT AND CELL IDENTITY
… to adulthood. How they functionally preserve transcriptional silencing has been deeply explored in embryonic stem cells (ESC), but remains poorly described in an in vivo context. Taking advantage of Cre-dependent conditional knockout mouse models targeting distinct Polycomb activities in the …
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Determining the Roles that DICER1 and Noncoding RNAs Play in Endometrial Tumorigenesis
… at the MLH1 locus could be involved in MLH1 transcriptional silencing. I characterized the MLH1/EPM2AIP1 bidirectional promoter region in endometrial cancer and normal cell lines and found an abundance of forward and reverse transcripts initiating from a large region of nucleosome-free DNA in …
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Histone modification and the epigenetics of X chromosome inactivation
… and females. In mammals this occurs through the transcriptional silencing of the majority of the genes on one of the two female X chromosomes. The inactive X chromosome (Xi) differs from its active homologue in a number of ways, including the hypoacetylation of core histones, a common property of …
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Study of the role of plant nuclear envelope and lamina-like components in nuclear and chromatin organisation using 3D imaging
… chromatin environment contributing to the transcriptional silencing of repeated sequences a general mechanism needed for genome stability. Quantitative measurements of 3D position of chromocentres in the nucleus indicate that most chromocentres are situated in close proximity to the …
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Probing the Mycobacterial Cell Envelope: CRISPR interference-mediated investigation of two essential genes in the galactan biosynthetic pathway
… involved in galactan biosynthesis and generate transcriptional knock down of wecA (also known as rfe, MSMEG_4947) and rfbD (MSMEG_6369) in M. smegmatis, a widely exploited modal for mycobacterial studies. We show that knockdown of both wecA and rfbD did not show hypersensitisation to first-line …
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Inter-Organellar Nucleic Acid Communication by a Mitochondrial tRNA Regulates Nuclear Transcription
<p>Balancing metabolic demands with transcriptional output requires efficient communication between mitochondria and the nucleus. However, the mitochondrial factors that mediate signals to the nucleus remain poorly defined. In eukaryotes, the mitochondrial genome encodes transfer RNAs (mito-tRNAs) …
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Definition of The Landscape of Chromatin Structure At The Frataxin Gene In Friedreich’S Ataxia
<p>Friedreich’s ataxia (FRDA) is caused by the transcriptional silencing of the frataxin (<em>FXN</em>) gene. FRDA patients have expansion of GAA repeats in intron 1 of the <em>FXN</em> gene in both alleles. A number of studies demonstrated that specific histone deacetylase inhibitors (HDACi) …
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The role of microvesicles in cancer and viral infection
… and flow cytometery data showed that transcriptional silencing of calpain by siRNA knockdown of CAPNS1 in PC3M cells prior to drug treatment inhibits MV release and results in induced apoptosis in cells. This mechanism contributes to understanding the reasons for insensitivity to …
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EXPLORING MITOCHONDRIAL AND REDOX VULNERABILITY IN FRIEDREICH'S ATAXIA USING GOLD NANOCLUSTERS IN HUMAN CELLULAR MODELS
… recessive neurodegenerative disorder caused by transcriptional silencing of the FXN gene, leading to frataxin deficiency, impaired iron–sulfur cluster biogenesis, mitochondrial dysfunction, and chronic oxidative stress. Despite significant advances in the understanding of FRDA pathophysiology, …
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EZH1 variants disrupt neurogenesis in hPSC-derived neuronal models and cause neurodevelopmental disorders
… Its paralogue, EZH2, is essential in maintaining transcriptional repression of non-lineage specific genes during development. In neurodevelopment, EZH2 is highly expressed in dividing cells, and its dysfunction leads to defects in neural progenitor proliferation and fate specification. In …
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Aberrant DNA methylation as a diagnostic and predictive marker of ovarian cancer
… of CpG islands (CGIs) is associated with transcriptional silencing of key tumour suppressor genes in cancer and is a frequent epigenetic event in epithelial ovarian cancer (EOC). The methylation status of 24 CGIs in a retrospective group of 142 EOCs and 16 non-tumour adjacent tissues were …
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RNA localizations of proteins that interact with the chromosome passenger complex
… of daughter cell inheritance of ncRNA mediated transcriptional silencing by showing that multiple ncRNAs disperse from the nucleus after nuclear envelope breakdown in prophase, and also that MCAK is implicated in Xist dispersal. These are important steps towards a comprehensive understanding of …
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Aberrante DNA-Methylierung des Transkriptionsfaktors C/EBP[alpha] bei akuter myeloischer Leukämie
… changes as an alternative mechansim of transcriptional inactivation of cancer-related genes in hematopoietic malignancies as well as the relevance of CCAAT/enhancer binding protein alpha (C/EBP alpha) in early stages of myeloid differentiation and regulation we investigated the …
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Investigating the molecular dynamics of gene re-silencing following treatment with epigenetic therapies
Aberrant transcriptional silencing of genes by promoter hypermethylation is an important mechanism of tumour suppressor gene (TSG) inactivation in cancer. DNA hypomethylating agents such as 5-Aza-2’deoxycytidine (5-Aza-dC) are approved for the treatment of myelodysplastic syndrome and show promise …
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piRNA-dependent transcriptional gene silencing during Drosophila oogenesis and embryogenesis
… Panoramix (Panx) is recruited and induces transcriptional gene silencing (TGS) by connecting to the general chromatin silencing machinery of the cell resulting in changes of the epigenetic chromatin state, thus shutting down transcription. However, whether Panx acts on its own or if other …
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The Role of Maelstrom in the Drosophila piRNA Pathway
… to genomic instability. In Drosophila ovaries, silencing of these elements is tightly regulated through the establishment of heterochromatin which prevents TE mobilisation. Piwi guided by piRNAs to nascent TE transcripts, it recruits factors necessary for creating transcriptionally repressive …
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