Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Transcriptional Gene Silencing"”.
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Mechanisms of piRNA-guided co-transcriptional gene silencing
… transcripts where it regulates the formation of transcriptionally repressive chromatin states. As well as numerous chromatin modifying proteins, this mechanism depends on the Panx-induced co-transcriptional silencing (PICTS) complex, which contains Panx, Nxf2 and Nxt1. PICTS operates at the …
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piRNA-dependent transcriptional gene silencing during Drosophila oogenesis and embryogenesis
… effects of transposons, thus maintaining transgenerational genome integrity. In Drosophila melanogaster ovaries, piRNA-Piwi complexes localise to the nucleus and scan nascent transcripts for transposon expression by using complementary antisense piRNAs as guides. Following target engagement, …
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The role of RNAi genes in Neurospora crassa post-transcriptional gene silencing
Meiotic silencing was first identified in the bread mold Neurospora crassa (by our group and others) and has since been discovered in worms, mice, and humans. Neurospora is an excellent model organism in that it is inexpensive, has a relatively small number of genes, and a rapid life cycle with two …
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Characterization of the roles of Xrn1p in small-RNA-mediated gene-silencing pathways
RNA interference (RNAi) is a small-RNA-mediated gene-silencing pathway that is involved in viral defense, transposon silencing, heterochromatin formation, and post-transcriptional gene silencing. Most RNAi pathways are initiated by long, dsRNA which is processed by Dicer into small interfering RNAs …
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RNA-based therapeutic approaches for FTDP-17
Neurodegenerative diseases are linked to altered splicing mechanisms (Mills et al., 2012). Fronto temporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) is one such disease that stems from the differential splicing caused due to mutations in Microtubule associated protein tau (MAPT) …
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Studio dei meccanismi molecolari coinvolti nel determinismo sintomatologico di piante infette da virus e virus-satelliti
… transformed with a viral suppressor of plant silencing (HC-PRO), did not experience the occurrence of yellowing anymore and, therefore, hypotesized that changes in symptoms were due to plant post transcriptional gene silencing (PTGS) mechanism. In our case, inoculation of N. benthamiana plants …
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Molecular and Genetic Analysis of Novel Epigenetic Silencing Factors Dhp1, Lsd1, and Lsd2
Epigenetic mechanisms regulate when, where, and how an organism uses the genetic information stored in its genome. They are essential to many cellular processes, such as the regulation of gene expression, genome organization, and cell-fate determination. In following chapters, we use molecular and …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
RNA interference (RNAi) is a post-transcriptional gene silencing mechanism found in all eukaryotic organisms. It is characterized by a family of small non-coding RNAs, either endogenous (in the case of microRNAs) or exogenous (in the case of siRNAs), that inhibits gene expression …
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The Role of Maelstrom in the Drosophila piRNA Pathway
… to genomic instability. In Drosophila ovaries, silencing of these elements is tightly regulated through the establishment of heterochromatin which prevents TE mobilisation. Piwi guided by piRNAs to nascent TE transcripts, it recruits factors necessary for creating transcriptionally repressive …
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The Mechanism of Double-Stranded RNA Response in Neurospora
… interference (RNAi) pathway, resulting in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and animals. In addition, dsRNA can trigger the interferon response as part of the immune response in …
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Regulatory Mechanism of the RNAi Pathway
RNA interference (RNAi) is post-transcriptional gene silencing initiated by Dicer, a RNase III that processes double-stranded RNA (dsRNA) precursors into small interfering RNA (siRNA). In Drosophila, Dicer2 and R2D2 coordinately recruit duplex siRNA to the effector RNA-induced silencing complex …
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Engineering a Photo-Control Mechanism for RNA Interference
… elicit potent and sequence-specific post-transcriptional gene silencing. Nucleic acid chemical modifications have been incorporated to improve their pharmacological properties. Despite numerous developments in chemical modifications for increased stability, safety, and efficiency of these …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… been linked to degradation of viral RNA by post-transcriptional gene silencing (PTGS), which is characterized by the accumulation of small interfering RNAs (siRNAs). In soybean plants infected with BPMV alone, siRNA accumulation was correlated with accumulation of BPMV genomic RNAs, but siRNA …
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Function and Regulation of Xylem Cysteine Protease 1 and Xylem Cysteine Protease 2 in Arabidopsis
… during these final two processes. Thus, the transcriptional regulation of XCP1 and the function of XCP2 were investigated. Qualitative and quantitative assessments of GUS activity as directed by various fragments of the XCP1 promoter showed that a 237-bp internal region was able to drive GUS …
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Characterizing RNA translocation in the parasitic weed Cuscuta pentagona
… beyond protein translation to include post transcriptional gene silencing or long distance signaling, and mobile RNA in C. pentagona systems offers novel insights into this aspect of plant biology. Studies of cell-to-cell trafficking of RNAs and other macromolecules would be facilitated by …
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Zur epigenetischen Inaktivierung der Wnt-Antagonisten SFRP-1, -2, -4 und -5 bei der Akuten Myeloischen Leukämie
… bone marrow suppression. In addition to numerous genetic aberrations in AML, it has become evident that epigenetic alterations play a significant role in the pathogenesis of AML. Silencing of tumor suppressor gene expression by aberrant CpG methylation in promoter regions is an established …
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NEW FINDINGS INTO TARGET-DIRECTED MICRORNA DEGRADATION MECHANISM
… of small non-coding RNAs that function in post-transcriptional gene silencing by interacting with target RNAs. A novel mechanism in control of miRNA levels, called Target-Directed miRNA degradation (TDMD), has been described, which involves specific transcripts able to interact with miRNAs and …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
… siRNA as a potent and selective mode of post-transcriptional gene silencing and has therapeutic potential in personalised medicine; however, the large size and negative charge of siRNA creates a hurdle for intracellular delivery that has thus far limited its development as a therapeutic. Cell …
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Identification and characterization of factors involved in DNA demethylation and anti-silencing in Arabidopsis
<p>DNA methylation is a key epigenetic mark for transcriptional gene silencing in many eukaryotes. DNA methylation status can be dynamically controlled by methylation and active demethylation processes. Compared to the well-known mechanisms of DNA methylation, the mechanisms of DNA demethylation …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein production) takes on particular importance due to the energy constraints imposed on the cell. However, the fate of …
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