Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 136 for “"Transcriptional Changes"”.
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The dissection of VEGFA stimulus-responsive regulatory and transcriptional changes in angiogenesis
… cues. To better understand the gene expression changes that occur in response to stimuli, I studied a time-course stimulation of human umbilical vascular endothelial cells (HUVECs) with vascular endothelial growth factor A (VEGFA) as a model system. Using data collected from multiple genome-wide …
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Multivariate analysis of transcriptional changes following restoration of SERCA2a levels in failing rat hearts
… underlying the control of gene expression at the transcriptional level, and the pathologic compensatory responses that occur in heart failure. The combinatorial control of gene expression that is thought to occur in mammals was implemented in a signal-processing model at the level of the heart …
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Toxoplasma gondii-mediated Host Cell Transcriptional Changes Lead to Metabolic Alterations Akin to the Warburg Effect
… high-throughput-sequencing, I examine host cell transcriptional responses to infection both at the mRNA and microRNA level, using two strains of T. gondii at a variety of Multiplicities of Infection over a time course of 43 hours. Through these transcriptional analyses I identify a number of …
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An investigation of the epigenetic and transcriptional changes which follow Epstein-Barr virus infection of germinal centre B cells
… a useful model for studying early EBV-associated changes contributing to the pathogenesis of HL. EBV infection of GC B cells is followed by the up-regulation of the DNA methyltransferases, DNMT3A, and the down-regulation of DNMT1 and DNMT3B, a pattern of expression which is re-capitulated in HL …
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Increased Oxidative Phosphorylation In An Ipsc- Derived Model of Rothmund-Thomson Syndrome Associated Osteosarcomagenesis
… that form osteosarcoma. We hypothesized that transcriptional changes that occur during differentiation may be important during osteosarcomagenesis. During differentiation, increased oxidative phosphorylation related genes, specifically transcription of genes which encode complex I of the …
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Peripheral blood mononuclear cells as non-invasive diagnostic indicators of stress-associated neural states
… states from human peripheral immune tissure transcriptional profiles, using microarrays. Although evidence in support of such an approach as a viable diagnostic avenue within psychiatric settings is accumulating, it remains to be demonstrated, in an animal model, that transcriptional changes …
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Insight Into the Metabolism of Lycopene: Focus on Androgen -Dependent Tissues
… in these tissues be involved in the observed transcriptional changes. In essence, lyc has previously been reported to improve antioxidant status but the presented data suggests that lyc could also modulate prostate cancer related genes.
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Studying the Effect of Low Doses of Ionization Radiation on Senescence in Human Lung Fibroblasts.
… exposure to normal human HFL1 cells and examined transcriptional changes. I found that HFL1 cells exposed to 10 mGy of gamma radiation had delayed senescence measured at 12 weeks post-irradiation compared to unirradiated cells. Through qPCR array analysis, I found that genes involved in human …
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Gene expression changes during mammalian male meiotic initiation
… However, a holistic assessment of the transcriptional changes during meiotic initiation has not been performed. In this thesis, we describe the transcriptional changes that occur during meiotic initiation in male mice. Previously, obtaining pure populations of male germ cells right …
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Early Onset Alzheimer’s Disease Markers in Mouse Hippocampus Unveiled by Single-Cell Transcriptomic Analysis Following Cranial Radiotherapy
… single-cell transcriptomics and DNA methylation changes as potential drivers of persistent cellular dysfunction after radiation exposure, specifically concentrating on the CA1-3 regions of the hippocampus and the prefrontal cortex due to their role in cognitive functions. Thirteen-week-old mice …
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Contextual Insights into the Rett Syndrome Transcriptome
… brain. A comparison between gene expression changes in the striatum, hypothalamus, and cerebellum of MeCP2-null mice revealed that gene expression changes are distinct between different brain regions, which suggests that MeCP2 function should be understood in a cell type-dependent context. To …
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STK25 and TAO Regulate Muscle-Specific Transcription and Muscle Function in the Parasitic Flatworm Schistosoma Mansoni
… not only do both stk25 and tao cause significant transcriptional changes, but those changes are unusually similar, supporting the model that these genes are interacting as part of a signaling pathway in the worm. RNAi-induced transcriptional changes were heavily biased to worm muscle, a clue to …
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Molecular mechanisms of microbial interactions in model systems and natural environments
… parasitic, and are mediated through nutrient exchanges and chemical signals. In this dissertation, I focus on characterizing molecular mechanisms responsible for interactions within two groups of microorganisms, phytoplankton and bacteria. In Chapter 1, I studied how extracellular metabolites of …
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CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
… know very little about the temporal dynamics of transcriptional changes and remodelling of the epigenetic landscape that occurs during the reprogramming process. To address this knowledge gap, I sought to develop an isolation strategy capable of identifying nascent naïve hPSCs early during …
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Investigation of endogenous and adoptively transferred T cell function in a genetic mouse model of lung adenocarcinoma
… these ends, we first sought to characterize the transcriptional changes leading to toward dysfunction in endogenous T cell responses. Using an autochthonous mouse model of lung adenocarcinoma, we longitudinally profiled CD8⁺ T cells from the lungs of tumor bearing mice using single cell RNAseq …
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Calcium-Stimulated Adenylyl Cyclases are Critical Modulators of Fear Learning and Experience-Dependent Plasticity
… is further supported by an overall decrease in transcriptional changes in DKO mice across several time points after conditioned fear: CF) learning, but most strikingly, at periods when memory consolidation and retention should be occurring. Since transcriptional changes are often dictated by …
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Dynamic Regulation of Metabolism in Archaea
… enzyme-coding gene targets, what the effect of transcriptional regulation is on metabolite levels, and why TrmB regulates so many metabolic processes in response to glucose. Using dynamic gene expression and TrmB-DNA binding assays, we found that that TrmB functions alone to regulate central …
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Probabilistic modelling of cellular development from single-cell gene expression
… sequencing can be used to investigate molecular, transcriptional, changes in cells as they develop. I reviewed the literature on the technology, and made a large scale quantitative comparison of the different implementations of single cell RNA sequencing to identify their technical limitations. I …
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3D Nanoscale Architecture and Physiology of the Human Airway Epithelium
Understanding how transcriptional changes lead to cellular-level architectural changes that drive cellular function is a central challenge in single-cell biology. An example of cellular remodelling is observed in the differentiation of airway basal stem cells into multiciliated cells within the …
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SPATIOTEMPORAL CONTROL OF THE SALT STRESS INDUCED TRANSCRIPTIONAL RESPONSE IN ARABIDOPSIS
… spatiotemporally under salt stress and how transcriptional regulation occurs. Firstly, I did an analysis on a previously generated spatiotemporal transcriptional map of salt stress in Arabidopsis roots, covering 4 core cell types and 6 time points for salt treatment. Based on this …
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