Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Transcription activation"”.
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The Role of DNA Binding in Transcription Activation by Estrogen Receptor
… a useful tool to study the mechanisms of gene activation by estrogen. XER/2AH exhibited 2-3 fold increased hormone-dependent transcription. Using a minimal promoter containing two copies of the estrogen response element (ERE) linked to a TATA box, it exhibited a 10 fold increase in …
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Mechanistic studies on transcription activation via DNA looping in a prokaryotic promoter-enhancer system
In this thesis, transcription activation was studied in a prokaryotic promoter-enhancer system. It comprises the E. coli RNA polymerase, which is associated with the alternative sigma factor sigma54 (RNAP-sigma54), and the transcription activator protein NtrC (nitrogen regulatory protein C), which …
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The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics
… gene's cis-regulatory sequence elements and the transcriptional output of that gene is fundamental to even the most complex network behaviors such as metabolism and differentiation. In eukaryotes, chromatin dynamics on gene promoter sequences is an integral part of regulation, and nucleosome …
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Structural and Functional Studies of the Unconventional Proteobacterial Transcription Activator Crl in Complex with the Transcription Machinery
… with the core RNA polymerase to initiate the transcription of genes needed to survive different environmental changes. Crl is a small protein that activates the transcription of σ<sup>S</sup>-dependent genes. In contrast to most transcription activators, Crl does not bind DNA to help recruit …
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Architecture of Escherichia coli promoters that respond to reactive nitrogen species
… promoter activity and the effects of the transcription factors that are reported to regulate expression of the hcp and ogt genes, namely NarL / NarP, FNR and NsrR. Transcription activation by NarL was then studied using semi-synthetic promoters. The hcp gene was found to be positively …
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Delineating Mechanisms of Signal-Induced RNA Polymerase II-Transcription
Signal-induced transcriptional programs regulate critical biological processes through the precise spatio-temporal activation of inducible gene programs. Understanding the dynamics by which RNA Polymerase II precisely induces transcription to activate these programs is important for dissecting the …
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The Novel Regulation of Histone Modification In Cancer Development
… physiological cues play important roles in gene transcription and cancer. However, the cellular signaling underlying this regulation is not well understood. Here, we show that, in a glucose dependent manner, E3 ubiquitin ligase NEDD4 ubiquitinates histone H3 on previously unstudied lysine (K) …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
… within the DNA sequence for processes such as transcription and replication. One of the mechanisms by which the genetic information can be accessed is post-translational modifications of the histone tails. Post-translational modifications, such as acetylation, act to neutralize charges on the …
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The role of Ten Eleven Translocation enzymes in the hair follicle mesenchyme
… of 5mC to 5hmC which in turn causes gene transcription activation. Dermal papilla (DP) cells located within the hair follicle are responsible for the regulation of development and the growth of hair follicles. Fgf20 signalling controls commitment of the mesenchymal precursor cells to the …
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Mechanism Of Transcription Regulation And Stability Of The Quorum Sensing Protein Trar
TraR is a transcription activator of the quorum sensing system in the plant pathogen Agrobacterium tumefaciens. This protein is composed of two domains: amino-terminal domain (NTD) which is responsible for binding the autoinducer OOHL and for dimerization and the carboxyl-terminal domain (CTD) …
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Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes
… moderately or severely defective at inducing transcription from most promoters, without dominant negative activity. This suppression of transcriptional activity may be mediated by an increased effect of nuclear receptor corepressors on E157D PPAR-gamma. In addition, the mutant binds atypical …
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Multicellular Phenotypic Studies of Single Gene Variants in Myxococcus xanthus
… three different methods: sequence homology, transcription activation and protemoics. The results from this research extended the list of <em>M. xanthus</em> genes involved in multicellularity, and expanded our knowledge regarding the possible molecular pathways underlying physiological and …
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Abundance and Localization of (Yes-associated protein) YAP in Prepubertal Bovine Mammary Tissue
… protein (YAP) and through downstream gene transcription activation, results in cell proliferation. Because YAP has never been identified in bovine mammary tissue, questions examined in this body of work mainly focused on the abundance and localization of YAP in mammary tissue of prepubertal …
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Controlling Gene Expression with Synthetic Molecules
… capable of functionally mimicking native transcription factors and regulating gene expression in a specific and predictable manner may represent a new paradigm in drug development. Native transcription factors are minimally composed of two domains, a DNA-binding domain (DBD) and an …
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Post-Transcriptional Regulation of Maternal mRNA Shapes Early C. Elegans Embryogenesis
… mRNAs, zif-1 and mom-2, are regulated post-transcriptionally through their 3’ untranslated region (3’ UTR) to ensure proper spatio-temporal protein expression. I discovered that seven RNA binding proteins bind directly to the zif-1 3’ UTR in a combinatorial fashion thereby ensuring that …
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The role of reactive oxygen species as mediators of respiratory syncytial virus induced pulmonary inflammation
… signaling molecules involved in STAT and IRF transcription factor activation and the IKK-epsilon pathway, two activated pathways involved in the regulation of pro-inflammatory gene expression. We showed that RSV induced oxidative stress in vivo and that antioxidant therapy with butylated …
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Structural and Functional Studies of Mycobacterial General Transcription Factors RbpA and CarD
… in bacteria is highly regulated at the step of transcription initiation. For decades, the vast majority of biochemical and kinetic studies have used Escherichia coli (Eco) RNA polymerase (RNAP) as a model for studying prokaryotic transcription initiation. However, properties of Eco RNAP are not …
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Detailed Analysis of Transcriptional Regulation by Activation Functions-1 and -2 of the Human Estrogen Receptor
… (ER), is a 66 kilodalton, ligand-inducible transcription factor that regulates the transcription of estrogen-responsive genes. Like other steroid hormone receptors, the ER is a modular protein that can be divided into separable domains with specific functions, such as ligand binding, …
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Investigating The Roles of The P63 Isoforms In The Microrna Biogenesis Pathway
… The <em>TAp63</em> isoforms contain an acidic transcription activation domain. The <em>ΔNp63</em> isoforms, lack the TA domain, but have a proline rich region critical for gene transactivation. To understand the functions of these isoforms, the Flores lab generated <em>TAp63</em> and <em>ΔNp63 …
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Expression, activation, and regulation of matrix metalloproteinase 2 (MMP-2) in the bovine corpus luteum
… The MMPs are mainly regulated at three levels, transcription, activation, and inhibition by endogenous tissue inhibitors of metalloproteinases (TIMPs). Unlike most MMPs, pro-MMP-2 activation is accomplished on the cell surface rather than extracellularly. The objectives of the present study were …
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