Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Transcript Profiling"”.
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Transcript Profiling of Soybean Seed Development From Fertilization to Maturity
… stages of development, while genes involved in transcription were often over-expressed at the later, desiccating stages of development. This may indicate the transcripts or products of these genes are stored in the quiescent seeds for use in early stages of imbibition and germination.
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Terpene analysis and transcript profiling of the conifer response to Heterobasidion annosum s.l. infection and Hylobius abietis feeding
… responses was investigated. Terpene and transcript profiles of Scots pine to H. annosum s.s. and H. abietis challenge were studied, and a customised oligonucleotide microarray with 36.5K cDNA elements designed based on the P. taeda transcriptome was used to study the Scots pine …
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Atvps34, the Arabidopsis Phosphotidylinositol 3-Kinase, Its Functions in ABA and Auxin Signaling Pathways and the Molecular Basis for Salinity Stress Tolerance in Extremophile Thellungiella Halophila
… that can survive in sea water. Microarray transcript profiling and intensity analysis, quantitative RT-PCR, and metabolite profiles revealed shared and divergent responses to salt stress in the two species. Sixty percent of regulated genes distinguished Thellungiella from Arabidopsis. …
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Clonal selection and characterization of epigenetic variation in Pichia pastoris
… characterization of these improved clones by transcript profiling should yield information about networks of genes central in heterologous protein secretion in the yeast P. pastoris.
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The anticancer mode of action of the quinone natural product cribrostatin 6
… of experiments including cell cycle analysis, transcript profiling, and analysis of reactive oxygen species (ROS) production, a hypothesis for the mode of action of cribrostatin 6 is developed. ROS generation is likely the primary mechanism by which cribrostatin 6 induces apoptosis in cancer …
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Development and Mechanistic Characterization of Novel Small Molecules as Cancer Therapeutics
… remains a challenging endeavor. Whole genome transcript profiling and genome-wide shRNA screens are tools that have recently become available to the researcher and their use in aiding mechanism elucidation is reviewed in Chapter 1. Also presented herein is the development and investigation of …
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Longitudinal gene networks, canonical pathways and functional analysis of microarray data from Longissimus thoracis et lumborum muscle samples of early-weaned Angus steers
… regulation in complex organisms relies partly on transcriptional control of gene networks as a long-term mechanism affecting the level of expression of several key enzymes. Objectives were to evaluate temporal gene expression profiles in Longissimus thoracis et lumborum of early-weaned (155 +/- 10 …
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Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period
… and 30 days relative to parturition (DIM) for transcript profiling via quantitative PCR. Estimated prepartal energy balance (EBAL) was greater (~159% vs. 102%, P<0.05) in OVE vs. CON, but during the first wk postpartum cows fed OVE prepartum were in more negative EBAL. Prior to calving, CON …
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Role of SMU Homologues in Pre-mRNA Splicing During Maize and Arabidopsis Development
… responsible for multiple mutant phenotypes. Transcript profiling of mto38/zmsmu2-1endosperm revealed that defective rRNA processing and inefficient proteinsynthesis in the mutant can explain the mutant endosperm phenotypes.Furthermore, splicing of multiple pre-mRNAs is altered in …
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To the Frontier of RNA Glycosylation: Novel Insights from Metabolic Labelling
… workflow, we have used high-confidence transcript profiling to identify distinct sets corresponding to each sugar probe, thereby highlighting the diversity of these sugar-RNA species. Collectively, this thesis provides a robust and critical understanding of the largely unexplored field …
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Dissection of Regulatory Networks Mediating Resistance and Susceptibility of Arabidopsis thaliana to the Downy Mildew Pathogen Hyaloperonospora parasitica
… virulent H. parasitica to colonize Arabidopsis. Transcript profiling revealed induction of auxin-associated genes in response to infection of Arabidopsis thaliana by virulent strains of the oömycete pathogen, H. parasitica. Experiments with the DR5
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Nuclear proteomics and transcription factor profiling in Chlamydomonas reinhardtii
The transcriptional regulation of the cellular mechanisms involves many different components and different levels of control which together contribute to fine tune the response of cells to different environmental stimuli. In some responses, diverse signaling pathways can be controlled …
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The relevance of adenylate levels and adenylate converting enzymes on metabolism and development of potato (Solanum tuberosum L.) tubers
… Whereas metabolite levels were hardly altered, transcript profiling of tubers with reduced apyrase activity revealed a significant upregulation of genes coding for extensins, which are associated with polar growth. The results are discussed in context of adaptive responses of plants to changes …
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Spatio-temporal analysis of florigenic signals in Arabidopsis thaliana, Sinapis alba and Brassica napus
… alba and Brassica napus in combination with transcript profiling by Affymetrix chip hybridization, metabolite profiling by gas chromatography – mass spectrometry and targeted protein analysis using antibodies. All analyses were performed on tissue-specific samples and focused on phloem sap or …
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Combinatorial biochemistry of triterpene saponins in plants
… five different plants. After targeted metabolite profiling confirmed the induction of triterpene saponin biosynthesis by methyl jasmonate treatment, a genome-wide cDNA-AFLP transcript profiling was carried out for the five plants. Taking into account the putative functional annotation and the …
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An Investigation of Plant Hypersensitive Response and Photosynthesis Inhibition on a Genomic and Physiological Scale
… P. syringae, (4) cross comparing the genomic transcriptional response to HR and PSII inhibiting herbicide and (5) studying expression of chloroplast-encoded genes in plants infected with P. syringae. In addition, transcript profiling of soybean in response to the shikimate pathway inhibitor, …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
… to probe cell cycle-regulation of histone gene transcription by introducing an HTA1 promoter-GFP reporter gene construct into an ordered array of ~4500 yeast deletion mutants. I scored defects in reporter gene expression for each mutant, generating a quantitative analysis of histone promoter …
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L-Carnitine Supplementation for Periparturient Dairy Cows
… 7,872 cDNA microarray was used for transcript profiling using liver samples obtained from experiment 1. Hierarchical clustering revealed that carnitine infusion altered hepatic gene expression patterns in cows fed for restricted and ad libitum feed intake. Expression of several …
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