Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Trans-complementation"”.
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The Role Of The Membrane Proximal Region Of The M2 Cytoplasmic Tail In Virus Replication
… alanine mutants were generated and analyzed in trans-complementation assays and recombinant viruses. The membrane proximal residues 46-69 tolerated numerous mutations with little, if any, affect on virus replication suggesting that the identity of individual amino acids in this region are less …
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Dominant Suppression of Early Innate Immune Mechanisms by Yersinia pestis
… developed a negative selection screen termed transposon site hybridization: TraSH) to identify bacterial factors required for the two different phases of <italic>Y. pestis</italic> pulmonary infection. I validated the technique by defining the essential genes for <italic>Y. pestis</italic> …
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Investigating the role of the West Nile virus and Koutango virus envelope protein in virulence phenotypes using structural protein chimeras
… different portions of the virus contribute to transmission and disease caused by these viruses, researchers have found ways to isolate viral components. While methods such as site-directed mutagenesis, virus-like particles, and trans-complementation have been useful, they all fall short of …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
… constructed in the C-terminal region of NS4B. Transient replication assays revealed that five mutants were incapable of replication, two displayed an attenuated phenotype, and eight exhibited replication levels comparable to the wild-type (wt) genome. Of the five non-replicating mutants, two …