Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"Trans-Golgi"”.
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Role of trans-Golgi network vesicles in mitochondrial fusion
… The results presented here indicate that trans-Golgi network vesicles (TGNv) are recruited to mitochondrial fusion sites and accelerate the speed of fusion. Super resolution live cell imaging revealed that TGNv are recruited downstream of the endoplasmic reticulum (ER), suggesting that the …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
The Trans Golgi Network (TGN) is the cell's central sorting station, and the complex trafficking patterns are organized by many types of trafficking adaptors. These include the heterotetrameric adaptor protein complexes (APs) and the monomeric Golgi-localized, gamma-ear containing, Arf-binding …
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Role of Adaptor Proteins in MPR sorting
Mannose 6-phosphat Rezeptoren transportieren lösliche lysosomale Proteine und sind daher essentiel für die Biogenese des Lysosomes. Sie binden die Enzyme im trans-Golgi Netzwerk und transportieren sie in Endosomen, wo sie aufgrund des niedrigeren pH-Wertes abdissoziieren. Die Rezeptoren kehren …
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Modification of a plant-based expression system to produce Lysosomal Acid Lipase (LAL) in plant cells using genetic modification of endogenous plant N-glycosylation
… cells, and localise to specific cisternae of the Golgi body. Only ST6GAL and GNTV localise in the medial/trans-Golgi but fusion of a sialyltransferase-derived N-terminal signal peptide is capable of targeting B4GALT1 to the medial/trans-Golgi. As a proof of concept for human therapeutic enzyme …
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Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications
… or yeasts. However, plants do not perform post translation protein modification in the same manner as mammalian cells, most critically in terms of N-linked glycosylation. This can impact both protein functionality and stability, key for human therapeutics intended for production in plants. This …
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Funktionelle Charakterisierung und ultrastrukturelle Lokalisierung der Isotypen des Hüllprotein-Komplexes Coatomer
… zwischen COPI- und Clathrin-vermitteltem Transport nahe. Während eine Vielzahl von appendage-Bindern im Clathrin-System beschrieben sind (Praefcke et al. 2004), waren im COPI-System ausschließlich ArfGAP2 und ArfGAP3 als g1-COP-Binder bekannt (Watson et al. 2004). In dieser Arbeit konnten …
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σ1-adaptin - the Small Subunit of the Clathrin Adaptor Complex AP-1
… der an der Bildung von Clathrin-Vesikeln am trans-Golgi Netzwerk und an Endosomen mitwirkt. Durch die Bindung an Phosphatidylinositol-4-phosphat, an ARF-1 und an Sortierungssignale der transportierten Proteine gelangt der Komplex an die Membran. AP-1 selbst bindet wiederum Clathrin, das zur …
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Identifikation des Transportproteins PACS-1 als Nephrocystin-interagierendes Protein
… In der vorliegenden Arbeit konnten wir das Transportprotein PACS-1 als Nephrocystin-interagierendes neues Protein identifizieren. Nephrocystin wird kodiert durch das NPHP1-Gen, welches bei Familiärer Juveniler Nephronophthise (FJN, NPH1) mutiert ist. Nephrocystin ist ein Multiadapterprotein …
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Einfluss posttranslationaler Modifikationen auf die Funktion des Prototyp Foamy Virus Hüllproteins
… lassen. So erfolgt beispielsweise die reverse Transkription des viralen Genoms nicht erst nach Eintritt in die Zielzelle, sondern, anders als bei Orthoretroviren, bereits in der Produzentenzelle noch während oder kurz nach der Morphogenese. Diese Eigenschaft teilen Foamyviren mit den …
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Yeast Dynamin Functions with Clathrin at the Golgi
… endocytosis, but also traffic from the Golgi to endosomes. In this study, the interdependency between Vps1 and clathrin was investigated. Subcellular localization of clathrin was at the Golgi, early and late endosomes, implicating clathrin in multiple trafficking pathways within the …
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The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
<p>Vps54 is a subunit of the Golgi-associated retrograde protein (GARP) complex, which is involved in tethering endosome-derived vesicles to the trans-Golgi network (TGN). The “wobbler” mouse is the phenotypic result of a destabilizing point mutation in Vps54. This mutation causes neurodegeneration …
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Double-stranded RNA uptake in Caenorhabditis elegans
… In the nematode Caenorhabditis elegans, a transmembrane protein named systemic RNAi deficient-2 (sid-2) is required for dsRNA uptake from the environment through the intestine. Furthermore, the tyrosine-protein kinase sid-3 promotes import dsRNA. However, the molecular mechanism underlying …
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Studies on the structural organization of Golgi complex
Golgi complex is a multi-compartmental organelle involved in posttranslational modification and sorting of secretory proteins. We have characterized the distribution in Vero cells of three Golgi cisternal membrane proteins, Sialyltransferase (SialylT, tans- Golgi/TGN), galactosyltransferase(GalT, …
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THE TRANSCRIPTIONAL PROGRAM OF GOLGI BIOGENESIS
The Golgi apparatus is considered a fundamental organelle that regulates various physiological processes. Due to its plasticity, it can expand during differentiation and in response to high secretory demand. However, transcriptional contribution to Golgi biogenesis remains poorly understood because …
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A novel principle for immunomodulator delivery with hematopoietic cells as vehicles Focus on soluble TNF-receptor gene expression, targeting to secretory lysosomes and regulated secretion
… hematopoietic cells as vehicles for targeting transgenic immunomodulators to the inflammatory sites. The work presented was focused on the anti-inflammatory soluble TNF receptor. A transmembrane soluble TNF receptor (sTNFR1) construct was expressed in hematopoietic cell lines. ER export was …
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Biological studies of organellar (Na⁺,K⁺)/H⁺ exchanger NHE7
… of NHE7 from the recycling endosomes to the trans-Golgi network (TGN). In agreement with this finding, I found that NHE7 can also be targeted to the plasma membrane and then internalized. Caveolins, structural proteins for caveolae, were identified as NHE7-binding proteins and it was …
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Exploring the Impact of Endothelial Bone Morphogenetic Protein Receptor 2 Loss on Phosphoinositide and Cytoskeletal Assembly Dynamics and the Pathobiology of Pulmonary Arterial Hypertension
… of intracellular trafficking between the trans-Golgi network (TGN) and plasma membrane (PM) is vital for protein secretion and growth factor signaling, which involves recycling and PM localization of cell surface receptors. These processes depend on the cytoskeleton and the presence of …
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A Chemically Induced Colitis Screen Reveals the Necessity for Membrane Traffic in Intestinal Homeostasis
… contributes to DSS susceptibility. TVP23B is a trans-Golgi protein that binds YIPF6. Both TVP23B and YIPF6 are necessary for the formation of secretory granules in goblet and Paneth cells of the intestinal epithelium. These studies reveal non-redundant molecules required for the return of normal …
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Investigating the Biological Functions of the Protein Kinase WNK1 in the Regulation of Cytoskeletal Structures and Membrane Trafficking
… vesicular trafficking between the Trans-Golgi network and late endosomes. Co-localization analysis suggested that WNK1 co-localized with AP-3 in a high pearson correlation coefficient (0.53). Depletion of WNK1 showed defect of the maturation of autophagosomes. Taken together, WNK1 …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… endosomes to late endosomes and then on to the trans-golgi network (TGN). Trafficking from late endosomes to the TGN is dependent upon an intact C-terminal tail and the retromer complex of endosomal coat proteins. Additionally, removing either 3 or 10 C-terminal amino acids from sst2 alters the …
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