Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Traction force"”.
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Dendritic Cell Migration and Traction Force Generation in Engineered Microenvironments
… detectors to develop a novel method for probing traction forces during chemotaxis. We find DC migration is driven by short-lived traction stresses at the leading edge or filopodia. We illustrate that spatiotemporal pattern of traction stresses can be used to predict changes in the direction of DC …
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
… elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the C2C12 …
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Development of a novel cell traction force transducer based on cholesteryl ester liquid crystals. Characterisation, quantification and evaluation of a cholesteryl ester liquid crystal based single cell force transducer system.
… resolution or ability to detect localised cell traction forces (CTF) due to their non-linear viscous behaviour under shear rates. A newly developed cell traction force transducer system based on cholesteryl ester lyotropic liquid crystals (LCTFT) was developed to sense localised traction forces …
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Upstream Migration Under Shear Flow: Force Generation and Molecular Regulation
… Despite the centrality of mechanical force to this process, the forces upstream-migrating cells generate at the cell-substrate interface have not been directly measured during sustained upstream migration, and the molecular machinery responsible for producing them remains …
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Biophysical and biochemical factors in the cellular microenvironment; effects on cell migration and invasion
… that as cells migrate they produce contractile forces called traction forces that are generated by the cellular cytoskeleton and transmitted onto the substrate. Yet the signaling mechanism that promotes this force production or how is it regulated is not well characterized. In attempt at address …
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Triggerable ligand presentation using caged-RGD
… real-time vinculin recruitment and cell traction force generation. These studies represented the first demonstration of triggerable adhesive ligand presentation in vivo and demonstrated the utility of caged-compounds for probing specific receptor-ligand responses on highly defined …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… that cells are also influenced by the mechanical forces and mechanical properties of the local microenvironment. Extracellular forces causing cellular deformation can originate from many sources, such as fluid shear stresses arising from interstitial or blood flow, mechanical stretching during …
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Experimental and Computational Analysis of Cell Mechanics during Spreading and Migration
… Cells use their ability to generate mechanical forces to probe the mechanical resistance of their environment and move forward. Biomechanical explanations for how mechanical factors in their environment affect these forces and how cells use their forces to migrate are still unclear. Here, using …
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Learning to visually predict terrain properties for planetary rovers
… that computes upper and lower bounds on the net traction force available at a patch of terrain, via a constrained optimization framework. Both model-based and sensor-based constraints are employed. A terrain classification method is proposed that exploits features from proprioceptive sensor data, …
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Phosphorylation Regulation of T-Lymphocyte Migration
… of Myosin-IIA enables it to generate contractile force inside a migrating T-cell, maintaining the shape and proper adhesion of the cell to extracellular matrix, both being requirements for successful migration. The regulation of L-plastin enables it to activate integrin adhesion molecules as well …
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Optimal Vehicle Path Generator Using Optimization Methods
… functions; minimum time and maximum tire forces. A three-degree-of freedom vehicle model is used to approximate the handling dynamics of the vehicle. Inputs into the vehicle model are steering angle and longitudinal force at the tire. These two variables approximate two requirements that …
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Harnessing the Regenerative Characteristics of Meniscus Superficial Zone Cells
… assays, mechanosensation analysis, and traction force microscopy. After, the matrix deposition potential of superficial cells was assessed on electrospun nanofibers. Here, we show at the transcriptional level that superficial cells are most similar to those of the meniscus body, while …
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Analysis and finite element approximation of an optimal shape control problem for the steady-state Navier-Stokes equations
… is applied. The split formulation decoupling the traction force from the velocity is proposed in conjunction with the penalized optimality system and optimal error estimates are derived.
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E-cadherin mechanotransduction beyond cell-cell junctions
… modified Magnetic Twisting Cytometry (MTC) and Traction Force Microscopy (TFM) coupled with fluorescence imaging. A major focus of Chapter 2 is the role of a cadherin-associated protein α-catenin in force transduction. MTC and TFM studies using α-catenin knockdown cells and knockdown cells …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… substrates were shown to be compatible with traction force microscopy (TFM). These represented preliminary results for a larger study to evaluate how microenvironmental stiffness and geometry influence cytoskeletal contractility and ultimately bone formation. Finally, a novel method was …
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Aging Changes Cell Mechanics and Dynamics with a Backbone of Cytoplasmic Crowding
… particle fluctuation, optical tweezers, and force spectrum microscopy, we demonstrate that aging halves motion inside the cytoplasm due to doubled stiffness, while active forces remain statistically similar. In addition, we take tomograms of cell refractive index that indicate a denser …
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Microfabrication Approaches for Understanding the Role of Vascular Mechanics in Progressive Diseases
… functional contractility using a single-cell traction force microscopy technique and developed a thin-walled arterial model for growth and remodeling response to mechanical perturbations. We found that amyloid beta induces a reduction in vascular smooth muscle cell mechanical output. We …
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Dissecting combinatorial microenvironmental regulation of cell fate and function using a multi-modal arraying platform
… hybridization and, crucially, cell-generated forces using traction force microscopy (TFM); and the deconvolution of signaling via cell–cell (ligand–receptor) interactions by combining cell-extrinsic ligand presentation with cell-intrinsic ligand knockdown. I delineate the use of this platform …
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Engineering Substrates for the Study of Cell Mechanical Interactions
… a novel approach to measure and map protrusive forces in cells and the application of fabrication techniques like wet etching and microcontact printing to answer fundamental biological problems. In order to isolate the effects of curvature from other factors, we have developed a technique to …
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