Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"TraR"”.
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Mechanism Of Transcription Regulation And Stability Of The Quorum Sensing Protein Trar
TraR is a transcription activator of the quorum sensing system in the plant pathogen Agrobacterium tumefaciens. This protein is composed of two domains: amino-terminal domain (NTD) which is responsible for binding the autoinducer OOHL and for dimerization and the carboxyl-terminal domain (CTD) …
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Genetic and Biochemical Characterization of the Mechanisms Concerning the TraR/AAI/TraM Mediated Quorum Sensing That Regulates Ti Plasmid Conjugation
… of four elements; the transcription activator TraR, the intercellular signal N-(3-oxo-octanoyl)-L-homoserine lactone, the cis-acting recognition site tra box located in the promoter region of the target gene and the antiactivator TraM that negatively modulates TraR activity. Using a combination …
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Characterization of an agrobacterial plasmid inducible for transfer by mannopine and evolution of the core replication and transfer functions of repabc plasmids with class i quorum-sensing and transfer systems
… quorum-sensing system involving three proteins: TraR, TraI, and TraM. TraR directly activates the transfer regulon but needs its ligand, an acyl-homoserine lacone quorum-sensing signal synthesized by TraI, to be active. One additional component, TraM, binds to TraR directly and inactivates the …
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Identification of Markers to Predict Benefit from Trastuzumab Treatment
… the development of a trastuzumab risk model (TRAR) able to identify patients with high- and low-risk of relapse. Application of TRAR model to available datasets and to a new series of HER2-positive BC patients treated with neoadjuvant trastuzumab designated TRAR as predictive of response …
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Cryo-EM Studies of Bacterial RNA Polymerase
… Eσ<sup>70</sup> bound to the F element-encoded TraR protein; and (4) Eσ<sup>70</sup>-dependent promoter DNA melting intermediates stabilized by the TraR transcription factor. Using cryo-EM, I captured RNAP structures that were intractable to crystallization, visualized multiple RNAP …
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Molecular Genetic Characterization of the Mating Pair Formation and Vegetative Replication Regions on the Agrobacterium Tumefaciens Ti Plasmid pTiC58
… the co-inducer of the transcriptional activator, TraR. The next 11 ORFs showed similarities with the trb genes of RP4, which encode mating pair formation (Mpf) functions essential for conjugation, as well as with other genes from the type IV secretion family. Expression of the Ti trb genes …
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Molecular genetic characterization of essential conjugal transfer determinants on the nopaline-type Ti plasmid from Agrobacterium tumefaciens strain C58
… in addition to the transcriptional activator, TraR and its coinducer, the N-acyl homoserine lactone molecule, AAI, control of gene activation in tra requires two other determinants. One regulatory determinant is the cis-acting promoter sequence, called the tra box, found in the intergenic …
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Molecular, Genetic and Physiological Studies on the Regulation of Conjugal Transfer of the Ti Plasmid pTiC58 by Agrobacterium Tumefaciens
… determined that quorum-sensing results from TraR and AAI activating transfer gene expression only at high cell densities. The bacteria measure their density by sensing the concentration of AAI in the growth medium.
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Engineering LuxR-type quorum sensing proteins for new functions
… factors (LasR, LuxR, RhlR, RpaR, and TraR) as well as the chimeric transcription factors developed in this work. Chimeric LuxR-type quorum sensing proteins and proteins for the sequestration of LuxR-type quorum sensing proteins provide powerful new parts to facilitate building …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… regulators LuxR from Vibrio fischeri and TraR from Agrobacterium tumefaciens was initiated. Previous studies demonstrated that in the absence of AHL, EsaR retains the ability to function as a weak activator of the lux operon in recombinant Escherichia coli. This thesis research further …