Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Total Internal Reflection Microscopy"”.
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The influence of vapor pressure on droplet contact dynamics on smooth surfaces.
… were performed with different fluids using Total Internal Reflection Microscopy (TIRM) on a smooth surface to gain insight into the mechanism for the air film failure of volatile liquids. Several parameters were varied including the impact velocity and droplet volatilities to understand the …
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Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
… in fusion events.</p> <p>Using superresolution microscopy to measure and track host–pathogen interfaces that occur on a scale below the resolution limit of the light microscope is becoming increasingly popular. I have used total internal reflection microscopy (TIRF) and Photoactivated …
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Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA
… the cell-attached capacitance measurements, the total internal reflection microscopy and by electrical ATP release measurements. It was found that global increase in [Ca2+]i promotes the formation of complexes of interconnected granules within cell cytoplasm that subsequently undergo exocytosis …
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Single-Molecule Characterization of Photophysical and Colloidal Properties of Biocompatible Quantum Dots
… confocal, epifluorescence and objective-type total-internal-reflection microscopy, were applied to observe surface-immobilized NCs. Fluorescence lifetimes were determined to be around 20 ns for single particles showing deviations from a mono-exponential decay. This observation was proven to be …
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An interdisciplinary approach to studying mechanistic, structural and toxic features of protein aggregates associated with neurodegenerative disorders.
… sensitive to membrane integrity are imaged using total internal reflection microscopy. It is shown that oligomeric species of the 42-residue form of the A$\beta$ peptide (A$\beta$42) are responsible for the membrane disruption. The methodology can be applied to the study of other proteins such as …
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Electric Field Manipulation of Gold Nanorods: Characterization of Far-Field Alignment and Spatial Positioning Through Optical Response Imaging Techniques
… length gold nanowires by using darkfield optical microscopy to track position. Other studies have characterized the alignment of nanometer length gold nanorods by observing changes in the absorbance spectrum. A remaining challenge is to develop methods to quantify successful alignment so that …
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Developing high-resolution methods to study DNA and RNA dynamics
… help of specifically designed DNA constructs and total internal reflection microscopy, we discovered that homodimeric SSB showed similar one- dimensional diffusion and salt-dependent binding mode transition behavior which was confirmed for intensively studied homotetrameric SSB, suggesting that …
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Polymer induced colloidal interactions : measured by direct and indirect methods
… colloids in bulk using direct methods, such as Total Internal Reflection Microscopy (TIRM), and indirect techniques, such as Static Light Scattering (SLS). The work was motivated by the observation that the physical properties of colloidal suspensions, e.g. solution structure and phase …
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Mechanism of regulation of human RAD51 recombinase through post translational modifications & mediator proteins
RAD51 protein plays an important role in homologous genetic recombination (HR), an essential DNA metabolic process used by cells to faithfully repair the most deleterious forms of DNA damage and maintain genomic integrity. RAD51 along with its bacterial counterpart RecA, bacteriophage UvsX and …