Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"Total Internal Reflection Fluorescence"”.
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Development of single molecule total internal reflection fluorescence microscope
… hidden in ensemble experiments. I have built a total internal reflection fluorescence (TIRF) microscope with single-molecule sensitivity to study fluorescent molecules. The microscope is modular and multifunctional with capabilities including a wide excitation wavelength range (400-2000 nm) both …
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Quantifying the Release of Protein Substrates from AAA+ ATPase ClpX by Single Molecule Total Internal Reflection Fluorescence Microscopy
… ATPase ClpXP. We present a new assay based on Total-Internal-Reflection Fluorescence (TIRF) microscopy that measures the lifetime of the ClpX-substrate complex. We demonstrate that the technique has the potential to identify factors that affect the mean lifetime of the ClpX-substrate complex. …
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Single-molecule imaging studies of protein dynamics
Single-molecule fluorescence imaging is a powerful method for studying biological events. The work of this thesis primarily focuses on single molecule studies of the dynamics of Green Fluorescent Protein: GFP) and other fluorescent-labeled proteins by utilizing Total Internal Reflection …
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The photophysics properties of lanthanide luminescent probes
… have been carried out using a time- resolved fluorescence assay (TRFA). Bi-cs124 was conbined with different chelating agents, specifically diethylene triamine pentaacetic acid (DTPA), ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and 3,6,9,12-Tetrakis …
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Investigation of peptide-lipid interaction by fluorescence correlation spectroscopy
… lipid model membranes was investigated using fluorescence correlation spectroscopy (FCS) and laser scanning confocal imaging. A similar mechanism of MV4s was observed as compared to V4. MV4s induced lipid aggregation before they disrupted the lipid membranes. By comparing between different …
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Super-resolution wide-field optical microscopy by use of Evanescent standing waves
… processes in biological systems. Optical fluorescence microscopy is an essential tool for investigations in many disciplines in biology and medicine with molecular specificity. The resolution of optical far-field microscopy has been limited by the wave nature of light. In this thesis, a …
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… occurs. Here, we present the single molecule fluorescence assay for probing the interaction between the ClpXP enzyme and its substrates. A covalently crosslinked ClpX hexamer maintain functionally stable form at the low concentration of single molecule level. Surface passivation through …
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… using single-particle tracking (SPT) with total internal reflection fluorescence (TIRF) microscopy. We found that the mobility of individual enzymes was enhanced three-fold in the presence of the substrate, and the motion remained Brownian. We showed that the relative increase in diffusion …
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Entwicklung eines fluoreszenz-optischen Evaneszenzfeldmesssystems für die Echtzeitanalyse von DNA-Mikroarrays
In dieser Arbeit ist das Messprinzip der Total Internal Reflection Fluorescence (TIRF) auf die Mikroarraytechnologie übertragen, angepasst und optimiert worden, um Hybridisierungsereignisse auf DNA-Mikroarrays in Echtzeit analysieren zu können. Dazu wurden verschiedene (mikro)-optische Auflicht- …
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Development of a TIRF Microscope for Single Molecule Binding Studies
A single-molecule total internal reflection fluorescence microscope (TIRFM) is necessary to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal …
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Domain Redistribution in Lipid Bilayers in the Presence of the Antimicrobial Lipopeptide Fengycin
… cyclic lipopeptide fengycin on model membranes. Total internal reflection fluorescence (TIRF) microscopy was used to visualize and analyse lipid phase separation within supported lipid bilayers (SLBs) of various compositions. The initial research investigated the influence of protocol parameters …
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Probing cellular protein complexes using single-molecule pull-down
… biomolecules at single-molecule resolution using total internal reflection fluorescence (TIRF) microscopy. We name this technology single-molecule pull-down or SiMPull. In one reification biotinylated antibody against the protein of interest is immobilized on a flow chamber. The flow chambers are …
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High resolution imaging and lithography using interference of light and surface plasmon waves
… lab, the standing wave surface plasmon resonance fluorescence (SW-SPRF) microscopy was developed. It is a combination of standing wave total internal reflection fluorescence (SW-TIRF), one of structured illumination techniques, with surface plasmon resonance (SPR). The SW-TIRF approach decreases …
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S-Acylation Is A Key Regulator of Orai1/Stim1-Mediated Store-Operated Calcium Entry In T Cells
… and mediate SOCE. Lastly, our studies using total internal reflection fluorescence microscopy showed that a lack of S-acylation of either Orai1 or STIM1 resulted in significantly reduced Orai1/STIM1 colocalization as evident from the diminished PM puncta formation. These data describe a novel …
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Potential-Dependent-Adsorption and Reorientation Responses Associated With a Phospholipid Monolayer at the Water /Dichloroethane Interface
Total internal reflection fluorescence (TIRF) and electrocapillary measurements were employed to interrogate the potential dependent mechanical and physicochemical properties of adsorbed films at the interface between two immiscible electrolyte solutions (ITIES). Mixed films of a phospholipid, …
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Super-Resolution Fluorescence Microscopy using Transparent Waveguide Chips
Super-resolution fluorescence microscopy (optical nanoscopy) allows us to surpass the optical diffraction limit and observe biological phenomena at the nanoscale using visible light. Different modalities come with their own trade-offs, such as imaging speed vs. achieved resolution or live cell …
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Investigating Spatiotemporal Kinetics, Dynamics, and Mechanism of Exosome Release
… cells (A549) using CD63 fluorescent probes and total internal reflection fluorescence (TIRF) microscopy. The kinetics of release, or loss of fluorescence post-fusion, can relay information about the fate of exosomes. Using kinetic analysis we determined that a portion of exosomes are free to …
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Microfluidic chips for combinatorial screening applications
… of detecting complimentary DNA fragments. Total internal reflection fluorescence (TIRF) microscopy is used for read out of the chip. Four virus-like oligomer targets with sequences corresponding to key fragments of the viruses HIV, HPV, Hepatitis A and Hepatitis B were tested against four …
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Protein segregation and conformation in antigen-receptor triggering: a quantitative fluorescence microscopy study
… distance affects antigen-receptor triggering. Total internal reflection fluorescence microscopy is used to establish that phosphatase exclusion decreases when intermembrane distance increases. This inverse relationship, demonstrated for both B cells and T cells, is consistent with the …
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