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Showing 1 to 17 of 17 for “"Topoisomerases"”.
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Dynamics of human DNA Topoisomerases I and II
… for investigating the cell biology of human DNA topoisomerases. Our most important finding was, that both types of mammalian topoisomerases are entirely mobile proteins that are in continuous and rapid flux between all compartments of the nucleus and between the cytososl and the chromosomes of …
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Studies on Type I and Type II DNA Topoisomerases From Mammalian Mitochondria
… of both nuclear and bacterial type I DNA topoisomerases. This enzyme, purified from bovine liver, requires cations as cofactors and possesses optimal activity at pH 7.0, 37°C and either 150 mM K+ or Na+ ions. Similar to nuclear enzymes and different from the bacterial enzyme, divalent ions …
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Type IA topoisomerases: a phylogenomic and biochemical analysis of Methanosarcina acetivorans C2A TopoIII
… that manipulate DNA topology. Since then, topoisomerases have been demonstrated to be vital instruments of the cellular machinery in a wide array of processes that are essential for life such as DNA synthesis, DNA repair and chromosome segregation. Their essentiality is further exemplified …
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Search for new types of deoxyribozymes and development of human topoisomerases inhibitors on the basis of oligonucleotides
… such as gap or mismatch next to the topoisomerases cleavage site are designed. Our studies demonstrated some of our newly designed oligonucleotides could inhibit human topoisomerases with high efficiency. The highly efficient inhibition could be caused by the covalent bond linkage …
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Specific Binding Affinity of the Non-Catalytic Domain of Eukaryotic Like Type IB Topoisomerase of vaccinia Virus
<p>Topoisomerases are ubiquitous proteins that alter supercoiling in double stranded DNA (dsDNA) during transcription and replication and. <em>vaccinia</em> and the closely related poxvirus <em>variola</em> virus, at 314 amino acids in length, encode the smallest of the type I …
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Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
<p>Topoisomerases are DNA-modifying enzymes found in prokaryotes, eukaryotes, viruses and organelles such as chloroplast and mitochondria. Information about these enzymes in eukaryotic systems is mostly limited to nuclear enzymes, although our laboratory has been characterizing the biochemical and …
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DNA FRAGILE SITE BREAKAGE AS A MEASURE OF CHEMICAL EXPOSURE AND PREDICTOR OF THE SUSCEPTIBILITY TO FORM CHROMOSOMAL REARRANGEMENTS
… in the generation of RET/PTC rearrangements. DNA topoisomerases I and II maintain structural integrity by recognizing and cleaving DNA secondary structures such as those at fragile sites. Co-treatment of human thyroid cells with APH and topoisomerase inhibitors significantly altered APH-induced …
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Investigating the mitotic roles of Topoisomerase 2 Alpha and Topoisomerase 2 Beta-Binding Protein 1 in the maintenance of chromosome structure and genome stability
Type II Topoisomerases are essential, evolutionarily conserved proteins, important throughout the cell cycle. They act by generating a transient double-stranded DNA break in one molecule and manoeuvring a second DNA segment through the resultant gap. This ability to catenate and decatenate DNA …
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OPTIMIZING GENE THERAPY: THE IMPACT OF SMALL MOLECULE MODULATORS ON ADENO-ASSOCIATED VECTOR TRANSDUCTION AND INTEGRATION
… etoposide, which specifically inhibit type II topoisomerases, are found to boost integration of AAV DNA. Using machine-learning inferences, we find that these integrations were found to be selectively favored near topoisomerase II binding sites. We also find that treatment with the SUMOylation …
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DNA supercoiling with a twist
… regulating or conserving DNA torsion. Topoisomerases regulate DNA supercoiling, which has been argued to provide a coherent explanation for the main modes of transcriptional control - stringent control, growth-rate control and growth-phase control during the normal cell growth. In this …
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Anticancer Potential of Soy Peptides
… activities of L1210 leukemia cells and human DNA topoisomerases. Bioactive peptides were isolated and identified by a co-immunoprecipitation approach coupled with nanoLC-Q-TOF-MS/MS. Soy protein hydrolysates produced by simulated gastrointestinal digestion induced L1210 cytotoxicity through …
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Síntese de derivados indólicos/acridínicos e avaliação da interação com dna através de técnicas de espectroscopia utilizando brometo de etídio como sonda fluorescente
… enzimas-chaves na proliferação celular, as topoisomerases. Com base nestas informações, este trabalho teve como objetivo a síntese de onze novos derivados indólicos com anéis 3-amino-2-tioxo-tiazolidin-4-ona, 2-tioxo-tiazolidin-4-ona, tiazolidin-4-ona e 2-tioxo-imidazolidin-4-ona condensados …
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Mechanistic Characterization of the Mitochondrial Type I DNA Topoisomerase and a Study of Genes Containing Type I DNA Topoisomerase-Related Domains
… different from known eukaryotic type I topoisomerases. Degenerate primers developed from eukaryotic type I topoisomerase conserved domains were unable to amplify a region of the mt-topo I gene in RT-PCR reactions, although the known nu-topo I was detected.</p>
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Aktive DNS-Topoisomerase II Alpha ist eine Komponente des salzresistenten zentrosomalen Strukturgerüstes
In einer früheren Arbeit berichteten wir, daß der monoklonale, für die DNA-topoisomerase IIalpha spezifische Antikörper Ki-S1 nicht nur die Kerne menschlicher A431-Zellen, sondern auch extranucleäre Strukturen färbt (Meyer et. al., 1997, J. Cell. Biol. 136, 775). Diese Strukturen waren …
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BIOMOLECULAR PRINTING FOR ARTIFICIAL LIFE-INSPIRED COMPARTMENTS AND SINGLE-CELL ARRAYS
… IB, a model protein for the Plasmodium topoisomerases, which are potential biomarkers for Malaria diagnosis. This new class of DNA-based functional substrates can be considered as a first step for the point-of-care diagnostic on a biochip platform for identification of molecular …
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Harnessing CRISPR-Cas9 screens to identify functional genetic interactions
The advent of CRISPR-Cas9 genetic engineering technologies has vastly improved our ability to interrogate genetic interactions in high-throughput studies. CRISPR-Cas9 screens have rapidly evolved to become the main approach for unbiased, genome-wide, forward genetic studies. This work seeks to …
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ΦΩΣΦΟΔΙΕΣΤΕΡΟΛΥΤΙΚΗ ΔΡΑΣΗ ΣΕ ΚΥΤΤΑΡΟΠΛΑΣΜΑ ΣΥΚΩΤΙΟΥ ΠΟΝΤΙΚΟΥ C3HB. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΤΕΣΣΑΡΩΝ ΕΝΖΥΜΩΝ
THE ACID RIBONUCLEOLYTIC ACTIVITY OF MOUSE LIVER CYTOPLASM IS DUE TO 2 RIBONUCLEASES. THEY ARE LOW MOLECULAR WEIGHT ENZYMES THAT RESIST ACID CONDITIONS BUT ARE DESTROYED AT 100 K FOR 10 MIN . THE ENZYMES SHOW DIFFERENT PRETENCES TOWARDS END-LABELLED NATURAL SUBSTRATES (55 RNA). RNASE I SHOWS THE …