Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Topoisomerase II"”.
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The role of topoisomerase II in amsacrine resistance
… including acridines, have been shown to induce topoisomerase II-associated DNA breaks both in cultured mammalian cells and in vitro in the presence of purified mammalian DNA topoisomerase II. However, mechanisms linking these protein-associated DNA breaks with drug cytotoxicity are poorly …
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Aktive DNS-Topoisomerase II Alpha ist eine Komponente des salzresistenten zentrosomalen Strukturgerüstes
… wir, daß der monoklonale, für die DNA-topoisomerase IIalpha spezifische Antikörper Ki-S1 nicht nur die Kerne menschlicher A431-Zellen, sondern auch extranucleäre Strukturen färbt (Meyer et. al., 1997, J. Cell. Biol. 136, 775). Diese Strukturen waren wahrscheinlich Zentrosomen. In dieser …
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Investigation of anthracycline based topoisomerase II inhibitors in triple negative breast cancer
… for treatment. Mitoxantrone (MTX), a topoisomerase II inhibitor belonging to the anthracenedione class, is used in BCa therapy. This thesis investigates the potential and mechanism of action of the MTX analogue KP71 and two metal organic frameworks (MOFs), UiO-66 and UiO-66-NH2, loaded …
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The physiological role of the two isoforms of DNA topoisomerase II in human cells
Unique functions of DNA topoisomerase IIalpha and IIbeta have been suggested. A human cell line which carries a homozygeous mutation of the nuclear localization sequence of the topoisomerase IIalpha gene expresses the isoform outside the nucleus at the onset of mitosis. At mitosis topoisomerase …
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Toxicidade genética das antraciclinas : associação entre estrutura química e ação inibitória sobre a topoisomerase II
… de compostos está relacionada à inibição da topoisomerase II (topo II) – uma enzima que atua no relaxamento da supertorção da dupla hélice de DNA, através da quebra e posterior religação de suas fitas - as diferenças observadas podem ser atribuídas ao mecanismo envolvido neste bloqueio …
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Characterization, Interaction and Chemoprevention of Potent Constituents From Grape Cell Culture
… culture extract and subfractions on human DNA topoisomerase II for cancer chemoprevention; (2) to characterize constituents in the most potent fractions; (3) to investigate interactions between bioactive polyphenols present in the most potent fractions; and finally (4) to assess cytotoxicity of …
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Synthesis, DNA interactions and activation of novel cytotoxic anthraquinones.
… with DNA and the DNA processing enzyme, topoisomerase II. Hence, an investigation of the DNA binding properties of mitoxantrone and related bis-substituted alkylaminoanthraquinones was undertaken with a view to developing agents with reduced toxicity to normal cells. DNA intercalation was …
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Brüche am MLL-Gen durch apoptotische Vorgänge im Zuge von Probenalterungen im Vergleich zu Brüchen im MLL-Gen bei t-AML
… Auch sollte der genaue Wirkmechanismus der Topoisomerase-II-Hemmer am MLL-Gen noch genuaer untersucht werden.
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DNA Secondary Structure in Human Disease and Gene Regulation
… predicted DNA stem-loop structure and predicted topoisomerase II cleavage. This suggests a role for DNA secondary structure formation in mediating topoisomerase II-dependent DNA breakage at sites with a propensity to form leukemia-causing gene rearrangements. We also examine quadruplex-forming …
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Anticancer Potential of Soy Peptides
… most common type of cancer in children. Various topoisomerase inhibitors are currently used in the treatment of leukemia. Epidemiological studies have associated soy consumption with lowering cancer risk. However, the anticancer potential and mechanism of soy peptides have not been fully …
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Identification and Characterization of Hells-CDCA7, a Nucleosome Remodeling Complex Required for Mitotic Chromosome Structure
… of mitotic chromosomes including histones, topoisomerase II and condensin are known to compact and shape the chromatin into rod-like chromatids. However, a complete picture of the proteins involved in shaping mitotic chromatin is unsettled. Here, I perform mass spectrometry on chromatin …
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The effect of doxorubicin on the U2OS cell cycle
… the cells. When a 10 [mu]M dose is administered Topoisomerase II is inhibited resulting in double stranded DNA breaks because the DNA is unable to relegate during synthesis. This is shown by lower levels of synthesis after analysis with Bro mo-2-deoxyuridine (BrdU) and Propidium Iodide (PI) …
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Combinatorial therapy for triple negative breast cancer and the effect of nanoscale surfaces
… an agent known to exert its effect through topoisomerase-II activation pathways. An additional incorporation of pentoxifylline, a methylxanthine derivative known for anti-metastatic effects in breast cancers, could further improve the efficiency. We used drug combination of nifuroxazide, …
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Klonierung und molekulare Charakterisierung von Deletionsbruchpunkten bei juveniler Nephronophthise Typ 1
… Als Mechanismus wird eine Beteiligung der Topoisomerase II diskutiert, da um beide Bruchstellen eine hohe Anzahl an Topoisomerase-Consensussequenzen gefunden wurde.
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Mechanisms of multidrug resistance in bladder cancer: the role of the nuclear membrane
… proteins, cytoplasmic vaults, alterations in<br/>topoisomerase II and increased expression of glutathione-S-transferases, all of<br/>which result in reduced chemotherapeutic efficacy.<br/><br/>Superficial bladder cancer is commonly treated with adjuvant intravesical<br/>chemotherapy using …
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OPTIMIZING GENE THERAPY: THE IMPACT OF SMALL MOLECULE MODULATORS ON ADENO-ASSOCIATED VECTOR TRANSDUCTION AND INTEGRATION
… drug classes. First, we evaluate the function of topoisomerase poisons, which are small molecules that stabilize topoisomerase complexes and DNA breaks. Second, we target SUMOylation. To date, small molecule inhibitors of SUMOylation have not been evaluated for AAV modulation, so we characterize …
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Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… strand break (DSB) and correlate to an abortive topoisomerase-IIb activity during spermiogenesis. When comparing the DSB frequencies at the two most fragile genomic loci (fragile sites FRA3B, FRA16D) in human and murine spermatozoa with lymphocytes, significantly increased DSB levels were …
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Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… strand break (DSB) and correlate to an abortive topoisomerase-IIb activity during spermiogenesis. When comparing the DSB frequencies at the two most fragile genomic loci (fragile sites FRA3B, FRA16D) in human and murine spermatozoa with lymphocytes, significantly increased DSB levels were …
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Spatially mapped gene expression analysis from tissue
… and characterized a novel RT-LAMP reaction for topoisomerase II alpha (TOP2A) mRNA biomarker for LNCaP prostate cancer cells."
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Lymphocyte-mediated drug nanoparticle delivery to disseminated lymphoma tumors in vivo
… liposome system to encapsulate the topoisomerase II poison doxorubicin, and a lipid-based nanoparticle system loaded with the topoisomerase I poison SN-38. We then generated in vitro-activated primary murine T cell carriers using optimized culture conditions that induced robust …
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