Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Top-down proteomics"”.
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Database support for top-down proteomics
Top-down proteomics is a revolutionary application for the identification and characterization of protein, known to be one of the most complicated and challenging issues in biology. In top-down proteomics, the quality and speed of the data warehouse is very important, as high accuracy results are …
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Development of a Top-Down Proteomics Platform for Characterizing Myelin Basic Protein Proteoforms
Pages x-xv are misnumbered as pages xi-xvi.
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Interrogation of Yeast Responses to Anaerobic Growth Conditions at the Intact Protein Level Using Improved Top-Down Proteomics
… and metabolic labeling quantitative strategy, top-down proteomics was employed to investigate the response of Saccharomyces cerevisiae towards anaerobiosis. Approximately 150 protein pairs were measured with 4 differential expressions determined including an aerobic and anaerobic isoform. …
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Top-Down Proteomics: Separation and Mass Spectral Characterization of Complex Cell Lysates From Methanosarcina Acetivorans and Human Cancer Cells
… data acquisition to be possible with top-down protein analysis. Taken together, these platforms represent the next step in increasing the utility of top-down.
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… proteins from a single run, the high-throughput Top Down proteome analysis of intact proteins is now a reality.
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Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
We applied this top down proteomics platform to study proteins from Methanococcus jannaschii and Saccharomyces cerevisiae. From the whole cell lysate of yeast, ∼120 ion species were successfully identified with 100% sequence coverage, and we also detected several post-translational …
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The complexity of bioinformatics: techniques for addressing the combinatorial explosion in proteomics and genomics
… by the data at hand. In particular, the field of proteomics often grapples with this challenge, due to being at the confluence of a large number of high-throughput data pipelines. This work presents a series of computational frameworks that address this challenge in a manner that is both …
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Proteomic Identification of Hemp (Cannabis sativa) Seeds by Tandem Mass Spectrometry and Evaluation of Cytotoxic Activity
… The study employed techniques, including top-down proteomics, size exclusion chromatography, affinity chromatography, high-resolution ESI QTOF LC/MS/MS, and De Novo protein sequencing bioinformatics tools. The process involved defatting cannabis sativa seeds in hexane and extracting …
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Development and application of OmpT-based middle down proteomics
… functioning of a biological system. The goal of proteomics is to provide such information as protein identities, post-translational modifications (PTMs) on proteins, and ultimately, quantification of proteins or their PTM levels from given biological samples, mostly using mass spectrometry. …
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Comparative Proteomic Analysis of Neem (Azadirachta indica) Seed and Leaves
<p>Modern research has significantly contributed to creating well-validated research data for the holistic use of the Neem tree in disease management. Neem’s astonishing historical and modern relevance, along with its small-molecule composition, provide further evidence for its selection as the …