Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"Toehold switch"”.
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Designing Next Generation Nucleic Acid Diagnostics Using Synthetic Biology and Artificial Intelligence
… the design of a novel CRISPR-Cas13 activated riboswitch which demonstrates the potential of synthetic biology tools for nucleic acid detection. Second, I describe the prototyping of a platform for the deployment of freeze-dried synthetic biology-based diagnostic assays in wearable formats, …
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Cell-free synthetic biology for affordable, on-demand diagnostics
… cell-free synthetic biology platform using RNA toehold switch sensors to detect RNAs from human gut microbiome. We showed that this method could quantify bacterial and human RNA transcripts comparably to gold standard methods while reducing time and cost. Next, I used similar cell-free detection …
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De novo designs of programmable toehold-mediated transcriptional attenuators
Engineered RNA-based genetic switches, such as riboregulators and riboswitches, are frequently utilized as translational and transcriptional regulatory elements to construct a diverse array of biological devices. RNA-only genetic circuitry provides several advantages over other types of genetic …