Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Tobacco etch virus"”.
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Evolvability of a viral protease: experimental evolution of catalysis, robustness and specificity
… engineering using the experimental evolution of Tobacco Etch Virus cysteine protease (TEV) as a model. I map key features of the local fitness landscape and characterise how they affect details of enzyme evolution. In order to investigate the evolution of core active site machinery, I mutated the …
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Identification of a novel HIV-1C protease from a microbial source
… of the E. coliscreening system using the Tobacco Etch Virus (TEV) protease, co-expression of HIV protease subtype C did not inhibit cell growth. Further investigations revealed PR C activity was inhibited by an endogenous E. coli protein. The inhibitor was isolated from E. coli crude cell …
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Epidemiology, Aphid Vectors, Impact and Management of Tobacco Etch Potyvirus in Hot Peppers in Jamaica
… is constrained by the aphid-transmitted potyviruses, tobacco etch virus (TEV) and potato virus Y (PVY). The virus epidemiology was not understood and no effective virus management system existed for these viruses. This study sought to identify possible management strategies for …
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The Consequences of Plant Anatomy on Viral Co-Infection
Plant viruses are infectious agents that exploit plants to survive, transmitted from host to host primarily by insect vectors. Within plant hosts, viral populations encounter both physical and physiological barriers that may influence their evolution. This work aims to identify opportunities during …
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EFFECT OF HISTIDINE TAG ON THE ENZYMATIC ACTIVITY OF PSEUDOLYSIN AND ITS INTERACTION WITH THE STREPTOMYCES METALLOPROTEASE INHIBITOR (SMPI) PROTEIN
… His tag was removed by digestion with tobacco etch virus (TEV) protease. Proteolytic activity of tagged or untagged PLN, monitored as the rate of release of <em>p</em>-nitroanilide (<em>p</em>Na) from <em>N</em>-succinyl-ala-ala-ala-<em>p</em>-nitroanilide …
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Engineering TEV Protease Specificity: An Exploration of Machine Learning and High-Throughput Experimentation for Protein Design
… useless. In this thesis, we aim to engineer TEV (tobacco etch virus) protease, a highly sequence-specific protease, to cleave alternative substrates. We incorporate novel high-throughput assays and powerful machine learning (ML) methods for highly effective protein engineering. The first portion …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… protein (MBP). A linker sequence encoding a tobacco etch virus protease (TEVp) cleavage site was introduced between MBP and DPAP1 such that TEVp cleavage would generate the presumed native N-terminus of DPAP1. Incubation of the MBP-DPAP1 fusion with TEVp resulted in the release of free …
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P1/HC-Pro aus dem Weizenstrichelmosaikvirus als Suppressoren des RNA silencing in Weizen
… verwendeten P1/HC-Pro des jeweiligen pathogenen Virus, nämlich Tobacco etch virus in Tabak und Turnip mosaic virus in A. thaliana, sollte untersucht werden, ob das ähnliche P1/HC-Pro aus dem Weizenstrichelmosaikvirus (WSMV, einem Tritimovirus ebenfalls aus der Familie Potyviridae) als Suppressor …
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Plant Virus Diagnostics: Comparison of classical and membrane-based techniques for immunoassay and coat protein sequence characterization for Cucumber mosaic virus and three potyviruses
… of pest management strategies. The virus diagnostic capabilities of several plant pathology collaborators within the Integrated Pest Management Collaborative Research Support Program (IPM CRSP) host countries were evaluated with the aid of a survey. Very few plant disease diagnostic …