Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Tn-mutagenesis"”.
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Transposon Mutagenesis Facilitates Discovery Of Genotype-Phenotype Associations and Functional Interrogation of the <i>Mycobacterium Kansasii</i> Genome
… the functionality of a phage-based transposon (Tn) mutagenesis system in <em>Mk </em>by generating and screening a library of random insertion mutants for altered macrocolony morphology. In this screen, we identified 41 mutants exhibiting unique phenotypes, among them one carrying disruption to …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony morphology and mycobacteriophage resistance, was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating Tn-mutant …
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Towards Novel Methods of Mutagenesis for Histophilus somni
… genes responsible for virulence, an efficient mutagenesis system is needed. Mutagenesis of H. somni using allelic exchange is difficult due to its tight restriction modification system. Mutagenesis by natural transformation in Haemophilus influenzae is well established and may be enhanced by …
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Disabling the intrinsic resistome of Mycobacterium tuberculosis: elucidating hierarchies of DNA repair and mutagenesis that undermine current antibiotic efficacy
… mc2 155, we applied genome-wide transposon (Tn) mutagenesis to identify conditionally essential repair pathways under treatment with genotoxins of different mechanistic classes. To this end, the DNA crosslinking agent, mitomycin C (MMC), and the gyrase inhibitor and clinically relevant TB …
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Whole-genome transposon mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria
… end, a combination of whole-genome transposon (Tn) mutagenesis and next generation sequencing (TnSeq) was applied in M. smegmatis ΔmetE, a gene-deletion mutant in which the cobalamin-independent methionine synthase is inactivated, rendering the cobalamin-dependent isoform, MetH, essential for …
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Determining the mechanism of protein transit through the peptidoglycan layer in Gram-positive bacteria and identifying phage-docking sites on Clostridium perfringens
… (Sec and pilus-dependent). Random transposon mutagenesis and several negative selection rounds were used to construct multiple mariner mutant libraries. These libraries were screened on specific agar to visualize respective toxin secretion with approximately 200,000 mutant colonies screened. …