Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Tn-insertion"”.
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Molecular-genetic characterisation of arginine auxotrophy in the opportunistic human pathogen, Pseudomonas aeruginosa
… of this further. I found that a transposon (Tn) mutant containing a disrupted argA gene was auxotrophic for arginine, but readily accrued secondary mutations enabling the mutant to grow, even in the absence of this amino acid. Whole genome sequencing revealed that one of these bypass mutants …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony morphology and mycobacteriophage resistance, was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating Tn-mutant …
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Whole-genome transposon mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria
… end, a combination of whole-genome transposon (Tn) mutagenesis and next generation sequencing (TnSeq) was applied in M. smegmatis ΔmetE, a gene-deletion mutant in which the cobalamin-independent methionine synthase is inactivated, rendering the cobalamin-dependent isoform, MetH, essential for …
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Determining the mechanism of protein transit through the peptidoglycan layer in Gram-positive bacteria and identifying phage-docking sites on Clostridium perfringens
Clostridium perfringens is a Gram-positive (Gram+) anaerobic spore-forming bacterium that can act as a lethal human pathogen. Depending on the pathway of entry and strain, infection can lead to gastrointestinal disease or gas gangrene. Disease, as with nearly all bacterial pathogens, is primarily …