Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Tn-Seq"”.
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Genetic Determinants of Salmonella and Campylobacter Required for In Vitro Fitness
… the genetic factors that increase the survival fitness of these foodborne pathogens will effectively help us formulate mitigation strategies without affecting the nutrition ecology. The objective of this study was to identify the genetic determinants of Salmonella and Campylobacter that are …
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Highly Saturated Transposon Sequencing identifies genes impacting Staphylococcus aureus pathogenesis in macrophages
… mutant libraries in S. aureus, and performed Tn-Seq to identify candidate genes with significantly altered abundance following macrophage invasion. While some significant genes were strain-specific, 107 were identified in common across both S. aureus strains, with most (n=105) being required …
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Investigation of Pantoea stewartii Quorum-Sensing Controlled Regulators and Genes Important for Infection of Corn
… and LrhA regulons were investigated using RNA-Seq. This led to the discovery of a potential regulatory interaction between them that was confirmed by qRT-PCR and transcriptional gene fusion assays. The involvement of LrhA in surface motility and virulence was also established in this project. A …
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Laboratory evolution and natural transformation in Acinetobacter baylyi ADP1
… – providing a potential explanation for the fitness benefit of competence loss. Chapter 3 describes studies aimed at improving transformation rates and better characterizing the ADP1 transformation system. We examined how perturbing factors that affect the fate of internalized DNA effects …
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Role of the Leucine-responsive Regulatory Protein during growth of the bacterial corn pathogen Pantoea stewartii subspecies stewartii in the xylem environment
… leading to wilting and necrosis. Previous Tn-Seq experiments provided insights into genes that are essential for in planta survival, including the master transcriptional regulator, Leucine-responsive Regulatory Protein (Lrp). To better understand the role of Lrp when Pss inhabits the xylem, …
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In planta studies of the corn pathogen Pantoea stewartii subsp. stewartii and applications of a corn-based industrial byproduct
… whole transcriptome study was performed via RNA-Seq to determine genes differentially expressed in the bacteria while inside the corn. It was found that nutrient transporters and stress response genes were upregulated specifically when the bacteria are in their host plant, suggesting a response …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… and utilization of the prophage- and insertion sequence(IS)-free *Corynebacterium glutamicum* platform strain CR101 for an efficient integration system, a transposon mutagenesis system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library …