Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Tissue specificity."”.
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Exploiting gene expression and protein data for predicting remote homology and tissue specificity
… and secondary structures; (b) the analysis of tissue-specific gene expression for predictive signals in the sequence and secondary structure of the resulting protein product; and (c) a study of ageing in the fruit fly, a commonly used model organism, in which tissue specific and whole-organism …
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Microfluidic-based Tools to Investigate Gene Expression and Regulation with Tissue-specificity during Aging in Caenorhabditis elegans
… and regulation. Gene expression is age-, tissue-, and individual-dependent so it is important to capture all these pieces of information. While there are pooled-sample techniques that can measure the entire transcriptome (e.g., RNA-sequencing), they fail to capture this gene expression …
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Characterizing blood plasma proteins from organ tissue in rainbow trout (Oncorhynchus mykiss) using a non-targeted proteomics approach
… reviewed and ~50% of both proteomes include tissue specificity, while >99% of the rainbow trout proteome lacks tissue specificity. This study aimed to expand knowledge on the rainbow trout proteome with a focus on blood plasma proteins. Blood, brain, heart, liver, kidney, and gills were …
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Sequence motifs predictive of tissue-specific skipping
… regulating gene expression. Motifs that regulate tissue-specific alternative splicing have been identified by groups studying small sets of genes. We introduce a tissue-specific skipping score for skipped exons using exon-exon junction microarray data. We compare these exons with known …
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A novel computational method for predicting tissue-specific disease-associated signaling pathways in human utilizing Caenorhabditis elegans reference data.
… a novel computational method is built to predict tissue-specific disease-associated signaling pathways in human by referring to C. elegans data. Tissue-specificity and disease association data are utilized to perform this prediction, with a support of a novel pathway finding algorithm. Lists of …
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EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT
… spermidine and spermine. In the target tissue of "Pneumocystis", the lung, levels of AdoMet were previously shown to be depleted experimentally using nicotine. Here we show that chronic administration of nicotine in an animal model of PcP resulted in decreased lung AdoMet content. Since …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
Liver-specificity of hepatitis B virus (HBV) replication has been attributed to the action of its second enhancer (EII). This thesis reports the characterization of EII and the subsequent isolation of a novel liver-specific DNA-binding protein which binds to, and activates EII. The cDNA clone of …
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Differential expression of small RNAs in soybean
… I selected some small RNAs that show tissue specificity based on results from high-throughput sequencing for further investigation. I optimized the protocols for small RNA isolation and blotting. Purified RNAs from multiple tissues such as immature seed coats and cotyledons, germinated …
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Oncogene-induced reprogramming of heterotypic cellular interactions
… frequencies in various cancers resulting from tissue specific mutational biases and natural selection. The factors contributing to the differential fitness of particular mutations in various tissue contexts is poorly understood. I hypothesised that cells harbouring different KRAS oncogenic …
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Zebrafish Models of KATP Channelopathies
… potassium (KATP) channels in mammalian tissues, as well as the molecular basis of the channelopathies that result from loss-of-function or gain-of-function mutations in these channels. These studies have also highlighted that the available pharmacology to treat such diseases is limited. …
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Medical Engineering and Medical Physics: Metagenomic Sequencing for Viral Diagnostics and Discovery
… higher doses are used to transduce non-liver tissues. In this work, we engineer specific amino acids on the viral capsid of AAV9 and use sequencing to screen millions of viral capsid variants to evolve an engineered AAV with up to 100 times higher muscle tissue specificity over natural AAV.
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Endogenous short interfering RNAs that silence chalcone synthase result in pigment pattern formation on soybean seed coats
… data from twenty-one samples in six different tissues including cotyledons, roots, stems, and leaves showed that CHS siRNA expression is limited to the yellow seed coats confirming the tissue specificity of the generation of the CHS siRNAs. The differential expression of CHS siRNAs during seed …
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Aggregation Pheromone Biosynthesis and Engineering in Plants for Stinkbug Pest Management
… epoxide products. This study investigated the tissue specificity and sequence of these conversions by performing crude enzyme assays with protein extracts from male tissues. Furthermore, candidate P450 genes were selected by RNA-sequencing and co-expression analysis and the corresponding …
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Bioinformatics study of mammalian MRNA polydenylation
… between human and mouse ortholog genes; (3) the tissue-specificity of the regulation of alternative polyadenylation in humans and mice; (4) the development of a novel approach to use SAGE data to study polyadenylation. A database is built to comprehensively document mappings of poly(A) sites in …
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Investigating Cell-Type-Specific Vulnerability in Mitochondrial Disease Using Stem Cell-Derived Neuronal Models
… genetic disorders that primarily affect tissues with high energy demands. Although disruption of oxidative phosphorylation is a shared hallmark across these conditions, their clinical manifestations display remarkable tissue specificity, with certain cellular populations showing …
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Towards a comprehensive human protein-protein interaction network
… challenges due to molecular complexity, tissue specificity, and alternate splicing. It therefore becomes important to build well-annotated benchmarks and accuracy measures to evaluate new data. Here, we describe three methods that provide a framework to build a comprehensive human …
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Modulation of Cerebral Artery Smooth Muscle BK Channel Function by Neurosteroids
… Moreover, these accessory subunits have tissue-specific expression, such that the β1 subunit is highly expressed within cerebrovascular smooth muscle and greatly increases the open probability of BK channels. Increased BK channel open probability is linked to increased dilation of …
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Analysis of maturation-specific genes in soybean seeds: Gene structure, desiccation induction and abscisic acid responsiveness
… acid stretch which might be related to the tissue-specificity expression or activity of these proteins. Possible roles of MAT proteins in seed maturation processes are discussed.
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THE DEVELOPMENT OF SELECTIVE PHOTOSENSITIZERS TO TARGET PATHOGENIC T CELLS AND PREVENT GRAFT-VERSUS-HOST DISEASE
… (GVHD) is caused by an attack on the recipient's tissues from donor allogeneic T cells. In cutaneous T cell lymphoma (CTCL), malignant T cells migrate throughout the skin to mediate the disease. Consequently, developing new therapeutics to selectively target pathogenic and malignant T cells may …
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Elucidating the Impact of Endogenous Proteins on Lipid Nanoparticle Organ-Targeting Outcomes
… (SORT) nanoparticles, an LNP platform for tissue-specific RNA delivery to therapeutically-relevant cells of the liver, spleen, or lungs after intravenous injection. I discovered that the biophysical class of a 5th lipid added to the LNP, termed a SORT molecule, alters the LNP's …
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