Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"Tissue Section"”.
-
Mass Spectral Imaging for Analysis of Pharmaceutical Ingredients
… lipids, and other bioactive species in tissue section/ Tissue prints. Although now widely accepted, there remains a host of competing and complementary techniques including DESI, MALDI, SIMS, and DART ionization techniques that allow the researchers to obtain appreciable ion intensities …
-
Deterination of the melanin content in the skin of albinos at the University Teaching Hospital, Lusaka, Zambia
… in 10% neutral buffered formalin, processed, and sectioned using a microtome then one tissue section was stained with H&E stain and the other tissue section was stained with the Mason-Fontana staining technique to determine the presence of melanin in the skins. The other second part, melanin was …
-
Deterination of the melanin content in the skin of albinos at the University Teaching Hospital, Lusaka, Zambia
… in 10% neutral buffered formalin, processed, and sectioned using a microtome then one tissue section was stained with H&E stain and the other tissue section was stained with the Mason-Fontana staining technique to determine the presence of melanin in the skins. The other second part, melanin was …
-
Fundamental aspects of imaging matrix assisted laser desorption ionisation mass spectrometry.
… distribution of molecules within a biological tissue section, without the need for radionuclide or fluorescent labelling procedures. The primary aim of the work presented in this thesis was to assess the use of a high repetition rate laser for MALDI-MS image analysis by developing methodologies …
-
HIV-Associated Neurocognitive Disorders: Role of Viral Reservoirs and Lipid Dysregulation
… detected with regular MALDI-MSI, using the brain tissue sections of the AD 3xTg mouse model. We proved that this assessment is compatible with subsequent immunofluorescence and histological analyses in the same tissue section. This approach is innovative and helpful in performing a multi-omics …
-
Laser ablation sample transfer for mass spectrometry
… imaging with IR laser ablation sampling, a thin tissue section placed on a microscope slide was scanned in two dimensions under a focused IR laser beam to transfer material to the target slide via ablation. After the material was transferred to the target slide, it was analyzed using MALDI …
-
Spatially mapped gene expression analysis from tissue
… analysis of spatial variation of an mRNA in a tissue section. Traditionally, this is accomplished by using in situ polymerase chain reaction (in situ PCR) or in situ hybridization (ISH). In addition, laser capture microdissection (LCM) followed by RT-qPCR of the locally captured tissue has also …
-
Deciphering and modelling the action of immune cells using highly multiplexed imaging and deep learning techniques
… host defense while limiting damage to host tissues, through an act known as selftolerance. T cells, their activation and their effector roles are of particular interest due to their prominent roles in antigen discrimination and subsequent cell-mediated immunity. However, there are diverse …
-
Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… these questions by firstly using human liver tissue from chronic liver disease to describe the morphological and transcriptomic changes observed in cholangiocytes and secondly, I take advantage of cholangiocyte organoids as an in vitro model to assess their plasticity in end stage cirrhotic …
-
Adamts9 is critical for the development of primary ovarian follicles
… We found adamts9 was widely expressed in various tissues during embryonic and larval development and transcripts are also maternally deposited. We found strong expression in the developing retina at 48 hours post fertilization (hpf), that shifted to the ciliary marginal zone at 72hpf. We also …
-
Method development for investigating chemical distributions in the nervous system using mass spectrometry imaging
… to a variety of biological samples ranging from tissues to dissociated cell populations, demonstrating the applicability of the approach for analyzing many systems. Sample preparation protocols are developed to prepare tissue sections for MSI. Multiple approaches are explored to improve ion image …
-
Changes in the Murine Nigrostriatal Pathway Following Pyrethroid and Organophosphate Insecticide Exposure: An Immunohistochemical Study
… to examine the possibility of non-degenerative tissue injury. Groups of matched treated/vehicle-control mice received three IP injections of the insecticide/dose of interest over a 2-week period. Counts of immunoreactive (IR) neuropil in the dorsolateral striatum were made from four pre-selected …
-
Ambient ionization - mass spectrometry: Advances toward intrasurgical cancer detection
… differences between normal and cancerous tissue were detected via direct tissue analysis in vitro by touch spray ionization (TS) or by analyzing sectioned or smeared tissue using desorption electrospray ionization (DESI). The physical form of the tissue, e.g. in vitro sampling, sectioned, …
-
The Direct Impact of Trimethelamine-N-Oxide on Cardiac Function
… and the direct impact of TMAO on heart tissue and cardiomyocytes has not been fully understood yet. In the first chapter, we reviewed the literature on TMAO-related atherosclerosis and cardiomyopathy to give us a general aspect of current research progress in the role of TMAO on CVDs. In …
-
Ultrasonic characterization of cell pellet biophantoms and tumors using quantitative ultrasound models
… function is estimated from the histological sections, and shown to have a similar shape to the theoretical structure function and to that calculated from BSC. The comparison between cell pellets and tumors of the same cell type suggests that the anatomic details in tumors must be taken into …