Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Timelapse Microscopy"”.
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Mechanisms of Gradient Tracking During Yeast Mating
… the cortex, directing growth toward that site. Timelapse microscopy of fluorescently-labeled polarity proteins revealed that the patch wandered around the cortex during gradient tracking. Mathematical modeling and genetic analysis suggested that fusion of vesicles near the polarization site …
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Cell Polarity Establishment in the Budding Yeast Saccharomyces Cerevisiae
… drugs Latrunculin A and B as well as high-speed timelapse microscopy of fluorescently labeled polarity proteins to characterize the assembly of the incipient bud site. </p><p>Often, ensuring that only a single axis of polarity is established is as important as generating asymmetry in the cell. …
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Investigating Transluminal Endothelial Bridging in a Microvessel-on-a-Chip
… wounds. Next, I undertook multipoint 3D timelapse microscopy of the microphysiological system which revealed the evolution of circumferentially orientated endothelial cell protrusions into transluminal bridges forming by partial delamination. I found that enhancing actomyosin tension by …
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Beyond Suspension: Light and Growth Dynamics in Immobilised Algal Cultures
… model of algal biofilm dynamics, the use of timelapse microscopy and single-cell tracking within microcolonies uncovered how external matrices and exogenous nutrient supply influence cell cycle and morphology. The findings revealed spatial heterogeneity among cells within colonies, providing …
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A Novel High Throughput Platform for Assessing Bacterial Growth and Morphology Enables Rapid Antibiotic Susceptibility Testing
… 96 agarose pads and relies on single-cell timelapse microscopy to image bacteria as they develop into microcolonies. The platform can be manufactured with a laser cutter at a low cost based on off-the-shelf parts, making it easy to adopt for other labs. A robust open-source segmentation …
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Chemomechanics of self-oscillating gels
… state of covalently bound Ru(bpy) 3 . Using timelapse microscopy, we recorded the BZ oscillations and tracked the far from equilibrium chemical behavior exhibited by the gels. At constant system temperature, the BZ reaction induced cyclic changes in the osmotic pressure of the gel, resulting …
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Smart microscopy methods for quantitative microbiology
… growth, and interaction. Widefield fluorescence microscopy, in particular, has revolutionised this field by enabling high-throughput, non-invasive, time-lapse observations of cellular processes at the single-cell level. However, despite its power, this method is not without limitations. Optical …