Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Time correlated single photon counting"”.
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Long-range depth profiling based on time-correlated single-photon counting
Single-photon detection technologies are of high relevance to light detection and ranging (LiDAR) applications for the range resolution and surface profiling of distant target objects. Modern single-photon detectors offer high quantum efficiencies and small timing jitters in the order of tens of …
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LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS
… Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, …
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Strategies towards high performance (high-resolution/linearity) time-to-digital converters on field-programmable gate arrays
Time-correlated single-photon counting (TCSPC) technology has become popular in scientific research and industrial applications, such as high-energy physics, bio-sensing, non-invasion health monitoring, and 3D imaging. Because of the increasing demand for high-precision time measurements, …
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Protein and RNA folding: from bulk towards high throughput single molecule experiments
… a design and implementation of a high throughput time correlated single photon counting experiment is presented. Drops of ~10 μm diameter are optically trapped and interrogated by a femtosecond probe laser beam. Fluorescence photons emitted by the sample inside the drop are collected in all 4π …
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Novel Thienyl Dyes and related Metal Carbonyl Complexes
… of the techniques of laser flash photolysis, time-resolved infrared (TRIR) spectroscopy and time correlated single photon counting. Chapter two involves the study of thienyl porphyrins. This chapter begins with a literature survey on the synthesis, photophysics and electrochemistry of thienyl …
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Time Resolved Light Sheet Microscopy
… to new methods of prevention and treatment. Time-correlated single photon counting fluorescence lifetime imaging microscopy is just one of the tools used to carry out this line of research. Here we demonstrate a direct interaction between two proteins involved in gene regulation and …
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The synergistic role of light and heat in liquid-based nanoparticle manipulation
… 3D temperature profiles were characterised by time-correlated single-photon counting microscopy, with a temperature-sensitive dye. Through analysis of the particle velocities, the thermoelectric field strength, as well as the previously unknown Soret coefficients of azide ions were quantified. …
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Development of a simulation environment for the analysis and the optimal design of fluorescence detectors based on single photon avalanche diodes
Time-resolved fluorescence measurements enable the study of structure of molecular systems and dynamical processes inside them. This is possible because of a very high sensitivity of fluorescence lifetime to the physical and chemical properties of micro-environment in which fluorophores are …
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Capillary and microchip gel electrophoresis using multiplexed fluorescence detection with both time-resolved and spectral-discrimination capabilities: applications in DNA sequencing using near-infrared fluorescence
… the information content obtainable from a single assay and system miniaturization has continued to be important research areas in analytical chemistry. The research presented in this dissertation involves the development of a two-color, time-resolved fluorescence microscope for the …
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RO-Based TDC Design with Configurable Resolution and Power for SPAD-Based TCSPC LiDAR Applications
Single-photon avalanche diodes (SPAD) are used to measure time-of-flight (ToF) using CMOS time-to-digital Converters (TDCs) for compact, low-cost, and highly integrated three-dimensional (3D) sensing. The TDC specifications are critical determinants of the system's range, resolution, and accuracy. …
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New approaches for optical and microoptical diagnostics in IC engines
… diffractive optical elements) has an about ten times better light collection efficiency than a commercial UV endoscope with simul-taneous high spatial resolution over a broadband spectral range. Its performance is demon-strated in various experiments. Microoptics for the generation of …
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High-speed electrochemistry using ultramicroelectrodes
… electrochemistry on microsecond and nanosecond time scales. Other researchers have used ultramicroelectrodes for this purpose, but their studies have centered on dissolved species and generally use cyclic voltammetry experiments. One purpose of this research was to look at new ways to apply …
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Design and Synthesis of Photoactive Metal-Organic Frameworks for Photon Upconversion and Energy Transfer Studies
… steady-state emission spectroscopy and time-correlated single photon counting (TCSPC) spectroscopy and are compared to those of the corresponding ligand in solution. Both the unique fluorescent properties of the ligand as well as individual framework structure, result in distinctive …
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Design and implementation of high linearity FPGA-TDCs and an integrated large scale TCSPC system for time-resolved applications
The time-correlated single-photon counting (TCSPC) technology is a vital, advanced measurement and analytical tool for time-resolved biomedical, physics research and many industry areas because of its high temporal resolution and sensitivity. Analogue-based conventional TCSPC systems have been …
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Luminescent Properties of Anthracene-based Metal-Organic Frameworks
… steady-state emission spectroscopy and time-correlated single photon counting (TCSPC) emission lifetime spectroscopy. The addition of carboxylic acid functional groups on the anthracene ring alters photophysical properties to varying degrees depending on the location and protonation …
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Synthesis and Characterization of pure-phase Zr-MOFs Based on meso-Tetra(4-carboxyphenyl)porphine
… defectivity and modulator properties. Chapter 3: Singlet-singlet energy transfer in PCN-223(free-base), a highly stable Zr-MOF based on meso-tetrakis(4-carboxyphenyl)porphyrin was investigated, using diffuse reflectance spectroscopy, steady-state emission spectroscopy, time-correlated single …
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Design, synthesis and characterization of photocage molecules for light-activated release: Applications in polymer chemistry and phototherapeutics
… control over the release of substrates over time. But most of the available photocages absorbed in the UV light region and hence showed cytotoxicity and poor tissue penetration. Therefore, there was always the need for photocages that will absorb in the wavelength range of 600-1000 nm, also …
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Exploring excitations and vibrations in semiconductor nanocrystals through fluorescence and Raman spectroscopy
… of QDs, including spectrally-resolved time-correlated single photon counting measurements to understand QD photoluminescence lifetime as a function of emission wavelength, and low-frequency Raman spectroscopy to measure acoustic phonons in nanocrystal solids. These two chapters serve as …
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New photonic imaging systems for biomedical applications
… but it is slow as it requires long pixel dwell times to measure long-lived phosphorescence. Other commonly used techniques include gated CCD and CMOS cameras, which lack single photon sensitivity and have a low frame rate. Moreover, the majority of the current PLIM systems use a microscopic …
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Konzeption und Umsetzung neuer Technologien zur biaxialen Winkelmessung und elektrooptischen Pseudostreckenmessung
Ein Ziel der Arbeit war die Entwicklung eines Verfahrens zur 3D-Positionierung auf Basis elektrooptischer Pseudostreckenmessung. Ein zweites Ziel war die Entwicklung eines Reflexgoniometers zur zweiachsigen Winkelmessung. Im Rahmen der Arbeit wurden die Grundlagen zur instrumentellen Umsetzung …