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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Time Resolved Fluorescence"”.
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Time resolved fluorescence of CdSe nanocrystals using single molecule spectroscopy
… some aspect of the temporal evolution of NC fluorescence tinder different conditions. In particular the methods of single molecule spectroscopy are used in many experiments allowing the behavior of individual NCs to be resolved from the blurring effect of averaging over the ensemble. Studies …
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A Time-Resolved Fluorescence Study of Silicon Nanoparticles: Testing the Dimer Stretching Model
The role of surface dimers in the fluorescence mechanism is explored using the theoretical framework of Allan, Delerue, and Lanoo [1]. Their single dimer computations are extended to a fully reconstructed structure using time dependent density functional theory. Despite the inclusion of a fully …
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Thermal and denaturation studies of the time-resolved fluorescence decay of superoxide dismutase
Time-resolved fluorescence of single tryptophan proteins have demonstrated the complexity of protein dynamic and structure. In particular, for some single tryptophan proteins, their fluorescence decay is best described by a distribution of fluorescence lifetimes rather than one or two lifetimes. …
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Picosecond Time-Resolved Studies of IVR: Theory and Applications of Time-Resolved Fluorescence Depletion
Time resolved fluorescence depletion (TRFD), a new technique for the study of intramolecular vibrational redistribution, is described. Picosecond time resolution is obtained through the application of picosecond laser pump-probe techniques. A time dependent perturbation theory based description of …
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Picosecond Time-Resolved Fluorescence Depletion Spectroscopy of Fluorene: IVR in the Excited Electronic State
This thesis presents the results of the time resolved study of intramolecular vibrational relaxation in the molecule fluorene. The results represent the first extensive study of a molecule using the technique which we have developed known as the time resolved fluorescence depletion technique. …
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Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, …
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Applications of pump-probe and two-photon techniques in time-resolved fluorescence miscroscopy and spectroscopy
… molecular phenomena. We developed a variation of time-resolved, pump-probe methodology using cross-correlation principles of the frequency-domain. An unique feature of our technique is that the low-frequency, cross correlation signal and its harmonics which contain lifetime information at high …
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Macromolecular crowding effects on the excited-state dynamics of novel FRET probes using time-resolved fluorescence
… in live cell studies. Recently, several fluorescence-based probes have been engineered to potentially quantify crowding within living cells. These dynamic probes contain a pair of fluorophores (mCerulean3 and mCitrine, the donor and acceptor, respectively) that are capable of fluorescence …
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The photophysical characterisation of a fluorescence-based immunoassay for the detection of gonadotropin-releasing hormone, type-1 (GnRH-I)
… semi-quantitative. This work presents a 'model' fluorescence-based homogeneous immunoassay for the detection of gonadotropin-releasing hormone, type-1, (GnRH-I) described by fluorescence spectroscopy and in particular time-resolved fluorescence techniques. In the model assay a new synthetic …
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Dynamics of biological macromolecules investigated by fluorescence depolarization
… to be important to their physiological function. Fluorescence depolarization is a powerful technique for the investigation of these dynamics. A general overview of the use of time-resolved fluorescence to investigate nanosecond dynamics is presented. The basic theory and experimental techniques …
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Dynamics of biological macromolecules investigated by fluorescence depolarization
… to be important to their physiological function. Fluorescence depolarization is a powerful technique for the investigation of these dynamics. A general overview of the use of time-resolved fluorescence to investigate nanosecond dynamics is presented. The basic theory and experimental techniques …
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Dynamic Aspects of Protein Structure - a Fluorescence Spectroscopic Approach
… were investigated using steady state and time-resolved fluorescence techniques. High resolution anisotropy decay measurements were used to test the findings of molecular dynamics calculations on tryptophan and tyrosine residues in lysozyme and bovine pancreatic trypsin inhibitor (BPTI) …
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Energy Transfer Among Fluorescent Molecules Dispersed in Thin Films of Polymer or Protein (Rhodamine B, Sulforhodamine 101, Polyvinyl, Poly (Vinyl Alcohol) Bovine Serum Albumin, Time-Correlated Photon Counting, Lifetimes)
Previous research using pulsed time-resolved fluorescence techniques has shown that because rhodamine B (RHB) has an appreciable overlap of absorption and emission spectra, a system of RHB molecules dispersed in poly(vinyl alcohol) (PVOH) can collect light energy and transfer the energy of the …
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Capillary and microchip gel electrophoresis using multiplexed fluorescence detection with both time-resolved and spectral-discrimination capabilities: applications in DNA sequencing using near-infrared fluorescence
… involves the development of a two-color, time-resolved fluorescence microscope for the acquisition of both steady-state and time-resolved data during capillary and microchip electrophoresis. The utility of this hybrid fluorescence detector has been demonstrated by applying it to DNA …
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Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples
… and chemical signalling. In this thesis, fluorescence-based studies were conducted on a fluorescent molecular rotor, BODIPY-Ci2, which was used to measure the intracellular viscosity. BODIPY-C12 is insensitive to the surrounding polarity using solvatochromic methods. It was demonstrated …
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Fluorescence Studies of Photosystem II Fluorescence Quenching During Desiccation
… to protect their photosynthetic machinery during times of desiccation. In hydrated conditions nonphotochemical quenching (NPQ) mechanisms are able to safely dissipate excess excitation energy as heat, but mechanisms of NPQ associated with desiccation tolerance are still largely unclear. In the …
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Local Viscosity and Environment on the Nanometer Scale
New optical setup for two photon excitation time-resolved fluorescence anisotropy and lifetime measurements was built and used to understand the local environment and viscosity. The rotational correlation time constants by the fluorescence anisotropy provide the information on the hydrodynamic …
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Early Events in the Folding of the Protein Ubiquitin
… pulse. The subsequent refolding is monitored by time resolved fluorescence lifetime and spectrum. In the case of stopped flow mixing, an initially chemically denatured protein sample is diluted into a refolding buffer at temperatures as low as -20°. Here X-ray scattering, circular dichroism, …
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The photophysics properties of lanthanide luminescent probes
… study of the luminescence quantum yield, lifetimes and the number of water molecules coordinated to lanthanide luminescent probes based on biphenylyl-7-amino-4- methyl-2(1H)-quinolinon (Bi-cs124) derivatives have been carried out using a time- resolved fluorescence assay (TRFA). Bi-cs124 was …
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Ultrafast linear and non-linear spectroscopy : from biological light-receptors to artificial light-harvesting systems
… in this work, linear and non-linear femtosecond time-resolved spectrsocopy were applied to investigate the structure-function and functiondynamics relationship in biological and artificially designed systems. The experiments presented in this work utilize femtosecond time-resolved transient …
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