Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 158 for “"Thrombin"”.
-
UNDERSTANDING GENERALIZED ALLOSTERY IN THROMBIN
Thrombin is a critical drug target for chemotherapeutic and antithrombotic therapy development. Although many experiments have demonstrated that thrombin is a multifunctional allosteric enzyme, the exact mechanism of thrombin's allostery is still unclear and widely debated. This dissertation …
-
Designing Allosteric Inhibitors of Thrombin
Thrombin is a key enzyme of the coagulation cascade exhibiting important roles in both pro-coagulation and anti-coagulation processes. Most clinically used anticoagulant drugs, including polymeric heparin, warfarin, hirudin, argatroban and the recently approved dabigatran, aim to reduce thrombin …
-
Structural And Functional Studies On Thrombin Allostery
Thrombin is an allosteric serine protease endowed with important physiological functions in the blood. Once activated, α-thrombin plays procoagulant, prothrombotic, and anticoagulant functions in blood. Its important role in blood coagulation can be emphasized by the fact that thrombin interacts …
-
Mathematical approaches to modelling and controlling blood thrombin formation
… community investigating the process of thrombin formation. Chapter 2 is a summary on what is known about the mathematical modelling of biochemical networks. There we present some of the formalism developed by Martin Feinberg and Rutherford Aris regarding structural aspects of such …
-
RADIOSENSITIZING EFFECTS OF THROMBIN PEPTIDE TP508 ON MEDULLOBLASTOMA CANCER STEM CELLS
… relapse. Recently our laboratory showed that thrombin peptide TP508, a novel 23-amino acid investigational drug, protects normal neural stem cells from ionizing radiation damage. To determine whether the peptide would also protect brain CSCs from radiation damage, I examined the effects of …
-
Role of Thrombin-Activatable Fibrinolysis Inhibitor in Breast Cancer Metastasis and Angiogenesis
… TAFIa is formed through cleavage by thrombin, plasmin or, by thrombin in complex with thrombomodulin. TAFIa is a carboxypeptidase, cleaving carboxyl terminal lysine residues from plasminogen binding sites on cell surface receptors, which are important in accelerating plasminogen …
-
Regulation of the Gene Encoding Thrombin-Activable Fibrinolysis Inhibitor in Non-Hepatic Cells
Thrombin-activable fibrinolysis inhibitor (TAFI) is a carboxypeptidase B-like pro-enzyme that, once activated, attenuates fibrinolysis. TAFIa also possesses anti-inflammatory properties. Although liver is the main source of plasma TAFI, platelet-derived TAFI has also been reported. An alternatively …
-
Immobilization Of The Anti-Thrombin DNA Aptamer: Attachment, Optimal Packing Density And Kinetics
… studies utilized the well-characterized anti-thrombin DNA aptamer. Investigations were conducted in order to ascertain effective methods of immobilization of the aptamer to silica and gold, the maximum packing density for which 100% of the aptamer activity is retained, and the binding kinetics …
-
Discovery Of Inactive E* Conformations In Thrombin And Other Vitamin K- Dependent Clotting Proteases
… studying the kinetics of Na+ binding to human α-thrombin, we observed a biphasic mechanism of binding whose analysis led to the discovery that in the absence of Na+, the enzyme exists in dynamic equilibrium between two conformations, E* and E. Structural and kinetic studies indicate that E is the …
-
Thrombin-Inhibiting Perfluorocarbon Nanoparticles: A New Class of Therapeutic for Acute Thrombosis Treatment and Diagnosis
… that a nanoparticle carrying potent direct thrombin inhibitors can advance the treatment of acute thrombosis arising from various cardiovascular pathologies. The thrombin-inhibiting nanoparticles presented herein are designed to focus the antithrombotic impact of direct thrombin inhibitors …
-
Factors affecting the thrombin inhibiting activity of heparin when immobilised to hydrogels by covalent bonding
Thesis (Sc. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1986.
-
Endothelial Signal Transduction Pathways Mediating Thrombin and Histamine Stimulation of NO-production. Role of AMP-activated protein kinase
… stimulation by the G-protein linked agonists thrombin and histamine with emphasis on the role of AMPK in maintaining normal NO-production. We demonstrated the presence of a PI3K-Akt independent pathway activating eNOS in HUVEC after treatment of cells with histamine or thrombin. We showed that …
-
Identification of a vascular smooth muscle cell transition associated with fibrous cap formation and induced by thrombin receptor activation
… caps of human plaques and its activation by thrombin in cultured human VSMCs induced expression of contractile, ECM and other genes characteristic of the fcVSMC state. Conclusions: My findings uncover a VSMC transition associated with fibrous cap formation in atherosclerosis that is also …
-
Molecularly Targeted Nanoparticles for Modulation of Inflammatory Mediators in Atherosclerosis
<p>The enzyme thrombin has been demonstrated through experimental and clinical studies to play a crucial role in mediating both inflammation and thrombosis in atherosclerosis. The cellular effects of thrombin in promoting atherosclerosis involve the activation of signaling pathways that result in …
-
Characterisation of protease activated receptors
The thrombin receptor is a seven transmembrane G-protein-coupled receptor that is activated by thrombin cleavage. In order to characterise the thrombin receptor three mouse monoclonal antibodies were isolated by using a recombinant fusion protein incorporating the extracellular domain of the human …
-
Effect of platelet factors and thrombin on multiplication of human bone marrow mesenchymal stem cells cultured in low and high oxygen level in vitro
… examine effects of platelet-derived factors and thrombin on multiplication of adult human mesenchymal stem cells derived from bone marrow (hBMMSC) in a low or in a high oxygen environment in vitro. Hypothesis: It is hypothesized that PDF will have no effect on multiplication of BMMSC cultured in …
-
FUNCTIONAL STUDIES WITH DIRECT ORAL ANTICOAGULANTS: INVESTIGATION OF THE REGULATION OF KEY BLOOD COAGULATION PROTEASES
… have shown that the central enzyme in clotting, thrombin, is sequestered inside of a clot, and protected from the natural downregulator antithrombin (AT). This is problematic for anticoagulants like heparin which depend on AT. Subsequently, it was found that the key upstream propagator of …
-
Signaling Induced by Inflammatory Mediators in the Rodent Pulmonary Microvasculature
… of interendothelial junctions.</p> <p>Thrombin is a well-studied mediator that has been shown to be barrier-disruptive rapidly increases microvascular permeability. Sphingosin-1-phosphate (S1P) is a more novel, less understood mediator that has been shown to mediate basal vascular …
-
Novel Sulfated 4-Hydroxycinnamic Acid Oligomers as Potent Anticoagulants
… creates a huge need for anticoagulation therapy. Thrombin and factor Xa have been prime targets for regulation of clotting through the direct and indirect mechanism of inhibition. This work investigates chemo-enzymatically prepared oligomers of 4-hydroxycinnamic acids (DHPs) as potential …
Page 1 of 8