Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Thiolation"”.
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The physiological consequences of loss of tRNA thiolation in Saccharomyces cerevisiae
… modifier, functions as a sulfur carrier in tRNA thiolation reactions. Urml is required for formation of the s2 moiety that is found as part of the more complex mcm5 s2 U34 modification on the anticodon wobble uridines of tGliUUC, of tGgluUUG and tLysUUU tRNAs in a variety of organisms. It has …
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An Activity-Based Proteomics Approach for Identifying Ajoene's S-thiolation Protein Targets in Blood and Cancer Cells
… probes for chemical biology investigation of S-thiolation in which the background theory of activity-based protein profiling and biotin affinity purification is presented. In the synthesis of these probes, chemical stability emerged as a major stumbling block. Gratifyingly, after several trials, …
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Novel polyelectrolyte complexes for oral insulin delivery.
… was found to be 72 plus-minus 2 mol%. Subsequent thiolation of Paa and QPaa involved carbodiimide mediated conjugation to N-acetylcysteine (NAC) and modification of the polymers using 2-iminothiolane hydrochloride. These two different thiolation procedures yielded their respective NAC and …
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Studies on stereospecific diketopiperazine oxidation and applications to the synthesis of complex epidithiodiketopiperazines
… of the product diols. An example of a subsequent thiolation was then demonstrated to proceed under retention of stereochemistry, in contrast to the stereoinvertive thiolations previously observed in several total syntheses. The data is supported by computational analyses. III. Progress Toward the …
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Biosynthesis and Functions of tRNA 2-Thiouridine in Bacillus subtilis
… and thionucleosides, such as 2-thiouridine. Thiolation of the wobble uridine on tRNA molecules to yield 2-thiouridine is critical for accuracy and efficiency in translation in all three domains of life, and depletion of this thionucleoside is associated with severe growth defects. Though its …
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The Glutathione S-Transferase GliG Mediates Gliotoxin Biosynthesis, not Self-Protection, in Aspergillus fumigatus: A Functional Genomic Investigation.
… with a gliotoxin biosynthetic pathway involving thiolation, which is mediated by the addition of the glutathione thiol group to a reactive acyl imine intermediate. A new reduction and alkylation assay, which uses sodium borohydride and 5’-iodoacetamidofluorescein to label gliotoxin, yields a …
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Development and application of new strategies for synthesis of complex diketopiperazine alkaloids
… ion intermediates necessary for nucleophilic thiolation of advanced diketopiperazines en route to potent epipolythiodiketopiperazine anticancer compounds. In this study, we develop an informative mathematical model using hydantoin derivatives as a training set of substrates by relating the …
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AKR1B8 IS A NOVEL MODULATOR OF CRYPTIC SELF-RENEWAL AND SUSCEPTIBILITY TO COLITIS AND ASSOCIATED CANCER
… Many protein enzymes are susceptible to S-thiolation induced by reactive oxygen species (ROS); and physiological thiols, glutathione (GSH) and free amino acid cysteine (Cys), are critical intracellular thiol antioxidants, protecting proteins from irreversible oxidative damage. AKR1B10 …
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Development of transition metal-catalyzed C-S and C-C cross-coupling reactions
… a general and highly efficient method for direct thiolation of heterocyclic C?H bond with thiols. Without the need of organohalide precursors, a variety of 2-thio-substituted benzothiazoles, imidazoles and indoles are synthesized in one step in the presence of CuI, 2,2'-bipyridine, and Na2CO3. …
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Two versatile cofactors, flavin adenine dinucleotide and non-heme iron, involved in DNA repair and natural product halogenation
… chlorination of L-2-aminobutyric acid bound to thiolation domain in the biosynthesis of yy-dichloroaminobutyrate.
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Novel Insights into the Regulation of Autophagy in Saccharomyces Cerevisiae
… oxidative stress, protein urmylation and thiolation of cytosolic tRNAs. Future studies will be required to reveal the exact mechanism through which cysteine inhibits SL-induced autophagy. I also discovered that autophagy can be significantly induced upon depletion of a Fe-S cluster …
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Mechanistic insights into the cancer cell cytotoxicity and blood stability of the garlic compound ajoene
… and proteomic binding study demonstrated S-thiolation between ajoene and the cysteine 93 residue in the -subunit of haemoglobin which may explain the observed blood instability. A biotinylated ajoene analogue was designed and synthesised to identify ajoene's protein targets within the …
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Development of Epipolythiodiketopiperazine syntheses and the total synthesis of diketopiperazine alkaloids
… as a highly diastereoselective oxidation and thiolation. Moreover, this divergent synthesis features a common aminothioisobutyryl intermediate that can be utilized to construct (+)- luteoalbusin A. The spectral data obtained from the synthetic samples confirmed the assigned structure for this …
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Speciation and microbial transformation of chromium, arsenic and mercury in municipal landfill leachate
… reduction of sulfate to sulfide and subsequent thiolation of the organoarsenic compounds in the MLL. The arsenic transformation in MLL demonstrated the extreme complexity of arsenic speciation. It highlighted the potential risk of human exposure of highly toxic thio-organoarsenicals and MMAIII …
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Effects of sulfur complexation on intestinal transport and toxicity of metalloids in cell cultures
… for the formation of inorganic thioarsenates, thiolation can be seen as a detoxification process due to decreased intestinal transport and cytotoxicity. In case of dimethylmonothioarsenate, which is known to form from dimethylarsenate, thiolation can be seen as an activation process due to …
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Development of novel formulations for mucosal delivery of protein based drugs
… non-annealed xerogels but were not affected by thiolation. Crystalline NaAc in the undialysed xerogel led to a three-fold reduction in BSA release. Significant reductions in BSA and INS release were also observed with the addition of EIs to TG-chitosan-BSA and TG-chitosan-INS xerogel …
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Investigation of the biosynthesis of the phosphonate antibiotics bialaphos and dehydrophos
… substrate. Both substrates were loaded onto the thiolation domain of PhsA as demonstrated by FTMS. The possible implications of these findings for the timing of the methylation reaction are discussed. Another phosphonate presented here is antibiotic dehydrophos, which contains a unique methyl …
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Identification & characterization of natural and synthetic compounds as new anticancer agents
… Z-ajoene interacts with Vimentin through a S–thiolation causing the disruption of Vimentin filaments and therefore an alteration of the cell morphology. Given that Vimentin is known to participate to the early stage of the metastatic process, I also investigated the potential effect of …
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Nitrogen-Oxidants Enable Group-Transfer Reactions
… to affect formal C(sp3)–H azidation, thiolation, trifluoromethylthiolation, deuteration, allylation, and vinylation to generate molecular diversity from a common intermediate. Building on this foundation, Chapter five documents a photoredox-mediated method to generate the requisite …