Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Thiolase"”.
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The biosynthetic Claisen condensation : mechanistic enzymology of biosynthetic thiolase from Zoogloea ramigera
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1987.
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Protein engineering and bioprospecting for selective hydroxyacid production in engineered Escherichia coli
… enzymes. Engineering efforts were focused on the thiolase enzyme, which catalyzes the first step of the 3HA pathway, while bioprospecting efforts were focused on the enzyme immediately downstream of the thiolase - the 3-ketoacyl-CoA reductase. Our computationally guided protein engineering efforts …
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Development and Optimization of an Assay of Scp-2/Thiolase Activity in Rat Liver, and a Study of the Substrate Stereospecificity of Permfe-2
… mechanism. Sterol carrier protein 2 (SCP-2/Thiolase) has recently been shown to harbor the peroxisomal thiolase activity responsible for cleavage of 24-oxo-THCA-CoA to C₃-CoA and cholyl-CoA. To characterize this enzyme further, an assay that is sensitive and specific is needed. An HPLC …
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Novel applications and methods for the computer-aided understanding and design of enzyme activity
… specificity in a key enzymatic step (the thiolase-catalyzed condensation of two acyl-CoA substrates) of an industrially useful de novo metabolic pathway. We present a model-guided. rational design study of ordered substrate binding applied to two biosynthetic thiolases. with the goal of …
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Molekulargenetische und reaktionskinetische Untersuchungen nativer und rekombinanter saccharolytischer Clostridien zur Optimierung der biologischen Wasserstoffproduktion
… proteins in E. coli BL21(DE3). Furthermore Thiolase was purified by affinity chromatography and its biochemical and kinetic properties were determined. The physiological condensation of acetyl-CoA was measured applying a coupled enzymatic assay where beta-hydroxybutyryl-CoA dehydrogenase …
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Computational modeling of knowledge and uncertainty in systems biology for drug target identification and protein engineering
… The second study uses a kinetic model of the thiolase and reductase enzymes of the 3-hydroxyacid metabolic pathway to interpret available in vitro data to determine the kinetic changes induced by a mutation in the thiolase. Sampling approaches cannot identify all combinations of rate constants …
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Antimycobacterial, docking and molecular dynamic studies of pentacyclic triterpenes from Buddleja saligna leaves
… pocket of Mycobacterium tuberculosis fadA5 thiolase compared to a reported inhibitor. Thus, their ability to inhibit the growth of M. tuberculosis was predicted and was confirmed to possess significant antimycobacterial activity when tested against Mycobacterium smegmatis, M. tuberculosis …
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Development of metabolic pathways for the biosynthesis of hydroxyacids and lactones
… through the expression of an acetoacetyl-CoA thiolase (thil, bktB, or phaA), a 3-hydroxybutyryl-CoA reductase (phaB or hbd), and tesB.
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Identification of new genes and pathways that act to delay C. elegans aging
… of premature aging. kat-1 encodes a ketoacyl thiolase involved in fatty acid beta-oxidation, suggesting that defects in fat metabolism can affect the regulation of aging.
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Microbial synthesis of 3,4-dihydroxybutyric acid, 3-hydroxybutyrolactone and other 3-hydroxyalkanoic acids
… of a novel activity associated with the thiolase enzyme and highlighted the biosynthetic capability of the platform. The experimental study of different pathway enzyme combinations offered insights into their activities and specificities to guide future enzyme selection. In another part …