Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Thiol/disulfide exchange"”.
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Thiol-Disulfide Exchange in Human Growth Hormone
… protein aggregation. The formation of non-native disulfide bonds is a common route to covalent aggregation of therapeutic proteins and other biologics [2, 3]. Disulfide bonds participate in hydrolytic and oxidative degradation reactions that form non-native disulfide bonds and other reactive …
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3-Hydroxy-3-methylglutaryl-coenzyme A lyase: investigation of cysteines mediating intersubunit disulfide formation and regulation by thiol/disulfide exchange and discovery of an extramitochondrial homolog
… the absence of reductant, cysteine 323 forms a disulfide bond with cysteine 323 on the adjacent monomer, blocking the substrate's access to the active site which results in diminished enzyme activity. The recently published crystal structure of the human enzyme indicates that cysteines 323 on …
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The role of ERO1 in oxidative protein folding in the endoplasmic reticulum
The formation of native disulfide bonds is critical for the folding and stability of many secreted proteins. We describe an essential S. cerevisiae gene, ER01, which encodes a conserved ER membrane protein required for disulfide bond formation in the er .doplasmic reticulum (ER). In a conditional …
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AKR1B8 IS A NOVEL MODULATOR OF CRYPTIC SELF-RENEWAL AND SUSCEPTIBILITY TO COLITIS AND ASSOCIATED CANCER
… roles in colonic diseases. The reversible thiol/disulfide exchange is an important regulatory mechanism of protein enzymatic activity. Many protein enzymes are susceptible to S-thiolation induced by reactive oxygen species (ROS); and physiological thiols, glutathione (GSH) and free amino …
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Synthesis and mechanistic studies into the cytotoxic activity of garlic-related trisulfides in cancer cells
… S-thioallylation of cysteine residues through a thiol-disulfide exchange reaction, we extend these findings to trisulfides, providing new insights into their potential as anticancer agents. Chapter One offers an overview of naturally occurring trisulfides, highlighting their significant …
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Novel Precursors for Polymer-Protein-Conjugate Synthesis via Reversible Addition-Fragmentation Chain Transfer Polymerization
… Conjugation of sulfhydryl-terminated PNIPAAm to thiol disulfide exchange reagents and maleimides was probed for later conjugation to proteins. Evaluation of the different cross-linking systems resulted in the choice of maleimides as cross-linkers for subsequent conjugation to the protein …