Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Thioflavin T"”.
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Novel sustained release formulation of leveodopa-dendrimer conjugate
… of AS was characterized quantitatively by using Thioflavin t-based fluorescence and Fourier Transform Infrared (FTIR) spectroscopy, and qualitatively by using light microscopy. Thioflavin t assay was chosen due its property to specifically bind to amyloid fibrils and as a result exhibits a higher …
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Field Amplified Sample Stacking on Amyloid Beta (1-42) Oligomers using Capillary Electrophoresis
… non-FASS conditions. Furthermore by conducting thioflavin-t (ThT) assays, it was observed changing the buffer concentrations in accordance to the FASS technique conditions did not effect the overall aggregation. Thioflavin-t (ThT) assays were also conducted in order to determine an agitation …
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Targeted magnetic nanoparticles for remote manipulation of protein aggregation
… electron microscopy image analysis and thioflavin T fluorescence spectroscopy were used to characterize the morphology and size distribution of aggregates before and after AMF stimulus. We found that the AMF stimulus is effective at destabilizing A[beta] deposits and causing a reduction …
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On the Kinetics of Protein Misfolding and Aggregation
… amyloid formation. In addition, it is shown that Thioflavin T, to-date the most frequently employed fluorescent label molecule for bulk solution kinetic studies, can in the presence of potential amyloid inhibitor candidates be highly unreliable as a means to quantify the effect of the inhibitor on …
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Thermodynamic Characterisation of Amyloid Fibrils
… of kinetic assays. Specifically, the use of Thioflavin-T (ThT) fluorescence monitored growth curves to quantify the kinetics of amyloid growth in a robust, high-throughput manner. The equivalent thermodynamic assays are largely underdeveloped and as such remain underutilised. This thesis aims …
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Development of peptide inhibitor nanoparticles (PINPs) for treatment of Alzheimer’s Disease
… effects on Aβ aggregation were determined by Thioflavin-T (Th-T) assay. In addition, a cell penetration assay was carried out in order to examine the ability of liposomes to penetrate into neuroblastoma SHSY-5Y cells. Results: Low concentrations of PINPs 0.1 μM inhibited Aβ aggregation and …
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Developing and Utilizing Gas-phase Laser Spectroscopy Techniques to Study the Effect of Desolvation on Molecules
… the structure. The photophysics of gaseous thioflavin T (ThT) in isolation and with select binding partners is examined in Chapters 5 and 6. The fluorescence enhancement (“turn-on”) response of ThT after binding, which is important in the effectiveness of ThT as a probe for amyloid fibrils …
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Amyloid seeding assays for the selective amplification of tau aggregates from human brain homogenates
… of the amyloid-sensitive fluorescent dye Thioflavin T (ThT). Recombinant tau fragments for seed amplification were designed based on structural and biochemical literature to include or exclude essential residues along the tau sequence to promote propagation of one class of tau aggregates …
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Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy
… fluorophore library based on the amyloid dye Thioflavin-T. Following on from this, is the presentation of novel bifunctional dyes capable of simultaneously identifying hydrogen peroxide and amyloid aggregates by combining existing tools for the independent detection of these species. The …
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Development of single-molecules techniques to study native α-Synuclein aggregates
… focused on utilising amyloid binding dye, thioflavin-T, to probe media samples for the presence of secreted αSyn aggregates. This involved studying aggregates using single-molecule confocal microscopy coupled with microfluidics to count and characterise thioflavin-T active species, as well …
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Studies of Structure, Dynamics, and Interactions of Amyloid Fibrils by Magic Angle Spinning Solid State NMR
… important molecules bound to amyloid fibrils, thioflavin T and microcrystalline precipitate formed by an amyloid related protein, ß2-microglobulin. In both solution and solid state NMR studies, we identified significant and specific chemical shift perturbations, agreeing well with previous …
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Biochemical and Structural Studies of Amyloid Proteins
… which is a method based on the fluorescent dye Thioflavin T (ThT) that reversibly bind to the aggregates and whose fluorescence increases upon binding. I firstly explored the use of other dyes for these experiments and found that a ThT dimer has higher affinity to α-Synuclein aggregates in …
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Directing the self-assembly of short peptide derivatives
… structure by staining the β-sheet fibres with Thioflavin T. The thesis concludes with a number of overall conclusions that can be drawn from the work presented, as well as some possible directions for future work.
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Developing peptide-based inhibitors of amylin aggregation as a novel treatment for type 2 diabetes
… of amylin aggregation as observed using a Thioflavin T (Th-T) fluorescence assay. The next generation of peptides, IO8 and RI-IO8, were assessed using Th-T, Congo red and transmission electron microscopy (TEM) techniques. IO8 (H2N‑RGANFLVHGR-NH2) showed strong inhibitory effects on amylin …
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A Bottom-Up Approach to Building an Artificial Cell by Self-Assembly
… the GUV was analysed using a fluorescent dye (Thioflavin T) and various modes of microscopy. While protein aggregation was observed inside the vesicles, harsh environmental conditions were required to induce this aggregation (e.g.heat, low pH). An approach to investigate protein condensation …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
… relevant conditions was demonstrated using thioflavin T binding, transmission electron microscopy and X-ray fibre diffraction and far-UV circular dichroism. Partial digestion of beta-casein fibrils and analysis by mass spectroscopy indicated a common amyloidogenic sequence in the P,Q rich, …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
… relevant conditions was demonstrated using thioflavin T binding, transmission electron microscopy and X-ray fibre diffraction and far-UV circular dichroism. Partial digestion of beta-casein fibrils and analysis by mass spectroscopy indicated a common amyloidogenic sequence in the P,Q rich, …
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Lessons from Tau RT–QuIC: Structure, Kinetics, and Drug Discovery
… of the amyloid–sensitive fluorescent dye thioflavin T (ThT). First, a heparin-free, salt-modulated RT–QuIC platform was engineered using two cysteine-free tau substrates, K12 and K11, encompassing the full repeat region. Under optimised conditions, specifically at the appropriate …
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The Identification and Development of Small Molecule Inhibitors of Amyloid β Aggregation
… libraries was performed using a conventional Thioflavin T spectroscopic assay. Such work identified another molecular scaffold capable of exerting a strong inhibitory effect against A$\beta$42 aggregation. A selection of analogues was synthesised to improve the in vivo profile of this library, …
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Modulating metalloproteinase activity with protein nanofibrils
… was assessed using a β-sheet binding dye, thioflavin T, and whey protein nanofibrils (WPNFs) were visually confirmed by transmission electron microscopy. Analysis of hydrolysates using matrix-assisted laser desorption/ionisation coupled with Fourier transform ion cyclotron resonance …
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